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A new sponge-associated starfish, Astrolirus patricki sp. nov. (Asteroidea: Brisingida: Brisingidae), from the northwestern Pacific seamounts

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A new sponge-associated starfish, Astrolirus patricki sp. nov. (Asteroidea: Brisingida: Brisingidae), from the northwestern Pacific seamounts https://t.co/MBBny1PMIn
Okay, now really important news: new species of starfish was named after Patrick from Sponge Bob because it’s associated with sponges https://t.co/jC2RKDgekK https://t.co/1MiSp9GVtg
RT @thePeerJ: Just published in #PeerJ - A new sponge-associated #starfish, Astrolirus patricki sp. nov. (Asteroidea: Brisingida: Brisingid…
Just published in #PeerJ - A new sponge-associated #starfish, Astrolirus patricki sp. nov. (Asteroidea: Brisingida: Brisingidae), from the northwestern Pacific #seamounts https://t.co/a2hpl8XiLF #biodiversity #marinebiology https://t.co/29ipg1zb0M
RT @batrach0spermum: Кто проживает в горах океана? Astrolirus patricki – семирукие морские звезды, обнаруженные на подводных горах Тихого о…
Кто проживает в горах океана? Astrolirus patricki – семирукие морские звезды, обнаруженные на подводных горах Тихого океана в компании губок, на которые иногда даже по трое забираются. Новый вид назван, конечно же, в честь Патрика, дружочка Спанч Боба.
Biodiversity and Conservation

Introduction

Seamounts are typical deep-sea biomes in the global ocean which harbor diverse types of habitats and benthic communities (Rogers, 2018; Victorero et al., 2018). Sponges are one of the dominant benthic fauna in seamount ecosystems, playing important ecological roles by providing habitat and settlement substrate for other seamount invertebrates, such as mollusks, hydrozoans and echinoderms (McClintock et al., 2005; Bell, 2008; Chu & Leys, 2010). Suspension-feeding brittle stars and crinoids with long and flexible arms are often observed perching on or wrapped around sponges (Hendler, 1984; Klitgaard, 1995; McClintock et al., 2005; Wulff, 2006). In this study, a new starfish species, Astrolirus patricki sp. nov., which was found attaching to deep-sea sponges, is reported based on five specimens from the northwestern Pacific seamounts.

Brisingidae species possess 7–20 spiny arms that are up to about 40 times the length of the disk radius (Downey, 1972; Downey, 1986). As exclusive deep-sea inhabitants, their long arms and spines potentially equip them to be excellent suspension feeders, stretching out and gathering food particles in the water column in the resource-diluted deep ocean (Emson & Young, 1994; Gale, Hamel & Mercier, 2013). Brisingidae is composed of 62 extant species designated in 10 genera (Mah, 2020). Genus Astrolirus Fisher, 1917 comprises only one species, Astrolirus panamensis (Ludwig, 1905), which was originally designated to genus Brisinga Asbjørnsen, 1856 and later proposed as the monotype in the new genus Astrolirus, differentiated from the other genera based on the presence of intercostal plates on arms and a pair of marginal plates between the first adambulacral plates (Ludwig, 1905; Fisher, 1917). This type species was discovered in the eastern Pacific Ocean at 1,820–2,418 m depth, with 1 eight-armed specimen and 27 nine-armed specimens of varying size (disc diameter 6–26 mm) reported (Ludwig, 1905). Thereafter, Astrolirus has seldomly been reported or investigated in the world ocean.

Astrolirus patricki sp. nov. herein described represents a new species in the genus. All five specimens of the new species are seven-armed and were captured from hexactinellid sponges (Fig. 1). Occasionally 2–3 individuals were spotted on the same sponge along with numbers of ophiuroids and crinoids. The new species differs greatly from A. panamensis in morphological characters and living habitat. In this study we present the taxonomic descriptions and illustrations of the new species, along with DNA barcoding sequences for each specimen. Phylogenetic tree for order Brisingida was constructed to incorporate the new species and new data from Hymenodiscus cf. fragilis (Fisher, 1906), Freyella cf. attenuata Sladen, 1889 and two Brisinga spp. on the basis of previous study (Zhang et al., 2019).

In situ photographs of Astrolirus patricki sp. nov.

Figure 1: In situ photographs of Astrolirus patricki sp. nov.

(A) Holotype RSIOAS044. (B) Paratype RSIOAS028. (C) Paratype RSIOAS003. (D) Paratype RSIOAS052. (E) Paratype MBM286625.

Materials & Methods

Sample collection and examination

During the COMRA (China Ocean Mineral Resources R & D Association) cruises DY31, DY37, DY41, DY56 and a seamount cruise in the northwestern Pacific Ocean seamounts from 2013 to 2019, five specimens of the new species (Table 1) were collected by mechanical arms or siphon-pumps equipped on HOV and ROVs. Specimens were photographed in situ and on board by digital cameras. Tube feet tissues were extracted from each specimen and frozen in −80 °C refrigerator or liquid nitrogen for later molecular experiments, while other parts of specimens were preserved in 100% ethanol for morphological examinations. Morphological identification was conducted under a stereoscopic microscope (Zeiss Axio Zoom.V16). The type specimens of the new species are deposited in the Sample Repository of Second Institute of Oceanography (RSIO), Ministry of Natural Resources, Hangzhou, Zhejiang, China. Paratype MBM286625 is deposited in the Marine Biological Museum (MBM), Institute of Oceanology of the Chinese Academy of Sciences (IOCAS), Qingdao, China.

Table 1:
Sampling information of Astrolirus patrickisp. nov. and four Brisingida specimens reported in this study.
Species Specimen voucher Cruise and station Collection site Depth (m) Collection date
Astrolirus patrickisp. nov. holotype, RSIOAS044 DY41B, MCROV06 Weijia Seamount, northwestern Pacific Ocean, 156.41°E, 12.47°N 1935 2017.9.21
paratype, RSIOAS028 DY37-I, Dive105 Weijia Seamount, northwestern Pacific Ocean, 156.78°E, 12.96°N 1581 2016.4.30
paratype, RSIOAS003 DY31-III, Dive73 Caiqi Seamount, northwestern Pacific Ocean, 154.98°E, 15.22°N 1807 2013.9.9
paratype, RSIOAS052 DY56, ROV12 RD Seamount, northwestern Pacific Ocean, 149.85°E, 13.36°N 2125 2019.10.10
paratype, MBM286625 FX-Dive70 M2 seamount near the Mariana Trench, northwestern Pacific Ocean, 139.42°E, 11.27°N 1458 2016.3.27
Brisinga sp.1 RSIOAS007 DY35-I, Dive83 Lamont Seamount, northwestern Pacific Ocean, 159.25°E, 21.61°N 1773 2014.7.29
Brisinga sp.2 RSIOAS023 DY31-III, Dive70 Caiwei Seamount, northwestern Pacific Ocean, 155.55°E, 15.93°N 2431 2013.9.4
Hymenodiscus cf. fragilis RSIOAS009 DY37-I, Dive104 Weijia Seamount, northwestern Pacific Ocean, 156.51°E, 12.65°N 1957 2016.4.28
Freyella cf. attenuata RSIOAS037 DY38-III, Dive143 Mariana Trench, northwestern Pacific Ocean, 141.97°E, 11.82°N 4783 2017.5.23
DOI: 10.7717/peerj.9071/table-1

DNA extraction, sequencing and phylogenetic analysis

DNA extraction method, primer sequences and PCR programs were as in Zhang et al. (2017); Zhang et al. (2019). Five barcoding genes of each specimen, COI (cytochrome oxidase subregion I), 16S rDNA, 18S rDNA, 12S rDNA and H3, were acquired and the sequences were deposited in GenBank database (see Table S1 for accession numbers). K2P (Kimura 2-parameter, (Kimura, 1980)) pairwise genetic distances were calculated for COI genes in MEGA6 (Tamura et al., 2013).

For phylogenetic analysis, sequences of Brisingida were obtained from GenBank and accession numbers were shown in Table S1. Stichaster striatus and Cosmasterias lurida from the sibling forcipulatacean clade to the Brisingida (Mah & Foltz, 2011) were chosen as outgroup taxa. New data from A. patricki sp. nov. and four Brisingida species including Hymenodiscus cf. fragilis, Brisinga sp.1, Brisinga sp. 2 and Freyella cf. attenuata were added in the analysis on the basis of the previous study. The combining dataset includes 21 genospecies from 10 genera of the order Brisingida and 2 outgroup species. Phylogenetic analyses were conducted for the concatenated sequences of all 5 genes as well as for each independent gene. Models of evolution were estimated using jModelTest (Darriba et al., 2012) and the GTR + I + G model was selected as the best fit model. Maximum likelihood tree was constructed in raxmalGUI (Silvestro & Michalak, 2012) using GTR + I + G model with 1,000 bootstrap replicates. Bayes analysis was conducted in MrBayes (Huelsenbeck & Ronquist, 2001) using GTR + I + G model, running for 3,000,000 generations and sampled every 1,000 generation to estimate the posterior probabilities. The first 7,500 trees were discarded as burn-in. The tree topologies were observed and edited in Figtree v1.4.3.

Nomenclatural acts

The electronic version of this article in Portable Document Format (PDF) will represent a published work according to the International Commission on Zoological Nomenclature (ICZN), and hence the new names contained in the electronic version are effectively published under that Code from the electronic edition alone. This published work and the nomenclatural acts it contains have been registered in ZooBank, the online registration system for the ICZN. The ZooBank LSIDs (Life Science Identifiers) can be resolved and the associated information viewed through any standard web browser by appending the LSID to the prefix http://zoobank.org/. The LSID for this publication is: urn:lsid:zoobank.org:pub:7C8A768D-1312-498E-8352-BC4116D0B0F0. The online version of this work is archived and available from the following digital repositories: PeerJ, PubMed Central and CLOCKSS.

Results

Systematics

Order Brisingida Fisher, 1928
Family Brisingidae G.O. Sars, 1875
Genus AstrolirusFisher, 1917

Diagnosis to Genus. Intercostal integument covered by thin plates; the first pair of adambulacral plates do not touch by their interradial faces, but are separated by a pair of marginal plates; first pair of marginal plates unit closely with a large interradial plate in the interradial faces.

Astrolirus patricki sp. nov.
urn:lsid:zoobank.org:act:0F238379-CD10-4E51-A74A-0FEF055D72A5

(Figs. 14)

Diagnosis. Arms 7, robust. Intercostal integument densely covered by irregular, abutting plates. No syzygy between proximal arm plates. The first pair of adambulacral plates separated by a pair of marginal plates. A large interradial plate above the first marginal plates, visible from the abactinal side, covered by scattered spinelets. Mouth spines and proximal adambulacral spines robust, densely distributed. Suboral spines 3–4; subambulacral spines 1–2, proximal ones truncate, capitate. One lateral spine to each adambulacral plate, starting from about the 8th. A pair of gonads to each arm.

Etymology. The name is originated from the character “Patrick Star” in the famous cartoon “SpongeBob Squarepants”, who always spends time with his best friend “SpongeBob”, a benthic sponge. Since all specimens of the new species were observed in situ living on sponges (Fig. 1), it was name by Patrick to reflect this curious relationship.

Material examined. Holotype, RSIOAS044 (Fig. 1A), 7 arms, of which 3 attached to the disk, others detached. r = 7.5 mm, the longest broken arm measures 153 mm without the missing tip. Length of genital area 35 mm, broadest part of arm measures 7 mm.

Paratype RSIOAS028 (Fig. 1B), 7 arms, including 3 regenerating ones, all attaching to the disk; r = 7 mm, the longest arm measures 153 mm, R/r = 21.9; length of genital area 36 mm, broadest part of arm measures nine mm wide. Paratype RSIOAS003 (Fig. 1C), 7 arms, all arm detached from the disk; r = 10 mm, R = 190–200 mm, R/r = 19–20; length of genital area about 50 mm, broadest part of arm 9–10 mm, at 15–20 mm from the disk. Paratype RSIOAS052 (Fig. 1D), 7 arms, r = 7.5 mm, the longest arm measures 168 mm, R/r = 22.4. Paratype MBM286625 (Fig. 1E), 7 arms, r = 7.5 mm, R = 171 mm, R/r = 22.8; length of genital area about 33 mm, broadest part of arm 7.5 mm.

Description of the holotype. Arms 7. Disk thick, elevated from the plane of arms (Figs. 2A2C). Abactinal surface of disk covered by small rounded plates (Fig. 2G) bearing multiple sharp, thin spinelets, with few scattered pedicellariae between and at the base of the spinelets (Figs. 2C, 2F). Madreporite body locates at the margin of the disk, elliptical with a curved rift in the center (Fig. 2C). Papulae absent.

Astrolirus patricki sp. nov., abactinal view.

Figure 2: Astrolirus patricki sp. nov., abactinal view.

(A) Paratype RSIOAS028. (B) Paratype RSIOAS003. (C), (D), (H), holotype RSIOAS044, (C) Abactinal surface of disk and proximal part of arms, with red arrow pointing at the madreporite body, white arrow at the interradial plate and yellow arrows at the marginal plates. The red frame indicates the proximal region of arm connecting the disk and genital region, where pedicellariae do no form regular costae. (D) Abactinal surface of arm genital area with mosaic plating, red arrows show the costae bands. (E) Paratype RSIOAS003, abactinal surface of arm genital area, red arrows show the costae bands. (F) Paratype RSIOAS052, zoom in view of the abactinal disk, showing the multiple sharp spinelets on disk plates. (G) Paratype RSIOAS052, a piece of dissected skin from abactinal disk, shot from the inner side of the skin, showing the small round disk plates. (H) Abactinal surface at the middle of arm, black arrows indicate the pedicellariae bands.

Abactinal surface of arm within genital area densely covered by abutting plates of irregular scale forms. Pedicellariae scattered in the proximal region connecting disk and the genital area, not forming costae (Fig. 2C). Costae present within the genital area, about 30 in number; costae thin, densely located (two to each adambulacral plate), composed of raised band of pedicellariae (Figs. 2D2E). Beyond the genital area, pedicellariae forms bands wider than costae, two to each adambulacrals (Fig. 2H). A pair of gonads to each arm. Each gonad with 3–6 oval pedals (Fig. 3G).

Astrolirus. patricki sp. nov. actinal view.

Figure 3: Astrolirus. patricki sp. nov. actinal view.

(A–D), (G), holotype RSIOAS044. (A) Actinal surface of the disk. (B) oral plates and spines; (C) Interradial angle between arms, red arrow shows the first marginal plates, yellow arrow shows the second marginal plate. (D) Lateral view of the disk, showing the conjunction of plates in the interradii. Red arrows show the first marginal plates, yellow arrows show the first adambulacral plates, white arrow shoes the interradial plate. (E) Paratype RSIOAS003, lateral view of the disk, red arrows show the first marginal plates, yellow arrows show the first adambulacral plates, white arrow shoes the interradial plate. (F) Paratype RSIOAS052, adambulacral plates and spines at the middle of arm, yellow arrows show the subambulacral spines, white arrows show the furrow spines, red arrows show the lateral spines. (G) One of the paired gonads and digestive caeca in genital area.
Astrolirus. patricki sp. nov. holotype RSIOAS044, mosaic image of abactinal arm.

Figure 4: Astrolirus. patricki sp. nov. holotype RSIOAS044, mosaic image of abactinal arm.

Yellow arrows show the subambulacral spines, white arrow shows the aboral furrow spine, black arrow shows the adoral furrow spine, red arrows show the lateral spines.

Adambulacral plate in proximal area subquadrate, elongated in middle and distal part of the arms (Fig. 4). Proximally the ambulacral groove almost completely concealed by adambulacral spines (Fig. 4). The first pair of adambulacral plates on adjacent arms entirely separated by a pair of marginal plates (Figs. 3C3D). No syzygy between proximal plates. The first pair of marginal plates unit closely with an interradial plate (Fig. 3D). Interradial plate large, nearly naked, extending to the abactinal side of disk (Fig. 2C). Proximally 2–3 marginal plates well-developed, present on the lateral side of the arm (Fig. 2C). The following marginal plates degrade to a line of protuberances on the side and corresponding to each adambulacral plates, bearing long lateral spine. Lateral spine present from about the 8th adambulacral plate, one to each plate (Figs. 3F, 4). The longest measures 15–20 mm.

Adambulacral plate armature includes: (1) 2 subambulacral spines and 1 aboral furrow spine, forming a diagonal row; the outer subambulacral spine usually the largest and most robust; (2) 1 adoral furrow spine, thinner than the aboral one (Fig. 4). The first pair of adambulacral plate bear a diagonal line of 4–5 spines, two on the surface of the plate, and 2–3 smaller ones on the distal furrow corner (Figs. 3B3D). At middle and distal part of arm, the number of subambulacral spine on each adambulacral plate reduces to 1 (Fig. 4). Proximal subambulacral spines truncate form, capitate (Fig. 3C). The furrow spines become shorter and thinner on distal plates. All adambulacral spines bear pedicellariae, leaving only the tip of the spines naked.

Mouth plate large, bearing 3–4 robust suboral spines, forming a diagonal line, similar in form with the proximal subambulacral spines (Figs. 3A3B); 2 small furrow spines present on the aboral corner of the plate, close to the first adambulacral plate; on the oral margin, the plate bears 2 very minute spines, one pointing to the actinostome and one pointing rather to the furrow, forming a sharp angle with the former. All oral spines bear pedicellariae.

Variations in paratypes. In the paratypes, adambulacral plates at middle and distal part of arm bear equal number of spines as the proximal adambulacral plates (Fig. 3F), instead of having one less subambulacral spine as in the holotype. In several undeveloped arms in the paratypes, gonads were not spotted. The shape and size of the marginal and interradial plates vary slightly in paratype RSIOAS003 (Fig. 3E).

Coloration. Color in life orange (Figs. 1, 2A2B).

Distribution. Known from the northwestern Pacific Ocean, on seamounts, 1458–2125 m depth.

Molecular and phylogenetic results. COI K2P distances between specimens of A. patricki sp. nov. are less than 0.003, which are considered to be intraspecific distances. A. patricki sp. nov. is closest to Brisinga species, with distances between 0.123–0.159. The topology of the Bayes tree is shown in Fig. 5 and the nodes marked by black dots are support by both Bayes tree and ML tree. This tree is overall in line with results of the previous studies (Mah & Foltz, 2011; Zhang et al., 2019).

Phylogenetic tree of order Brisingida including Astrolirus patricki sp. nov. and 4 new specimens based on a concatenated dataset of COI, 16S, H3, 12S and 18S genes.

Figure 5: Phylogenetic tree of order Brisingida including Astrolirus patricki sp. nov. and 4 new specimens based on a concatenated dataset of COI, 16S, H3, 12S and 18S genes.

Topology follows the result of Bayes tree, bootstrap values and posterior probabilities are shown for each node. Nodes marked by black dots are support by both Maximum Likelihood Tree and Bayes Tree. The new species and new data reported in this study are colored red in the tree.

Remarks for A. patricki sp. nov. Types of A. patricki sp. nov. from different seamounts show little intraspecific divergences other than size and adambulacral spine number. The difference between A. patricki sp. nov. and the type species of the genus, A. panamensis, are listed in Table 2. Although both species are characterized by the presence of abactinal plates, in A. panamensis the plates locate between the transverse costae, whereas in A. patricki sp. nov. a mosaic of abutting plates forms the abactinal arm skeleton. The two species further differ in arm number, adambulacral spine number and distribution, as well as the number of genital organs. It should be noticed that, in Ludwig’s original description of A. panamensis, “several, consecutive pairs of branched gonads on each arm (4–5 in one male specimen)” were recorded, each branch has their own gonopore (Ludwig, 1905), but later when Fisher established the new genus, he described Astrolirus as having 2–4 gonads to each arm (Fisher, 1917). Whether this new description resulted from a reexamination of the type specimens was not clearly indicated (Fisher, 1917). Therefore, in this study we choose to follow Ludwig’s original descriptions when evaluate the differences between the new species and A. panamensis.

Table 2:
Major morphological differences between A.patrickisp. nov. andA. panamansis.
Diagnostic characteristics A.patrickisp. nov. A. panamansis
arm number 7 8–9
costae raised bands of pedicellariae a transverse line of up to 11 strong, thick plates
intercostal plates abutting usually isolated by small distances
adambulacral plate armature 1–2 subambulacral spines, 1 adoral and 1 aboral furrow spines 1 subambulacral spine, 1 adoral and 1 aboral furrow spines in most specimen
lateral spines 1 to each adambulacral plate on every second or third adambulacral plate (in irregular change)
suboral spine 3–4 1
genital organ 1 pair to each arm several, consecutive pairs on each arm (4–5 in one male specimen)
DOI: 10.7717/peerj.9071/table-2

In addition, A. patricki sp. nov. is characterized by a large number of suboral spines (3–4), whereas in A. panamensis, only 1 suboral spine present on each oral plate. Interestingly, in A. panamensis, some large specimens (r = 13 or 10.5 mm) have the 2 suboral spines on a pair of oral plates covered in a same membrane (Ludwig, 1905). This characteristic has only been reported in Brisinga andamanica Wood-Mason & Alcock, 1891 and Brisinga bengalensis Wood-Mason & Alcock, 1891 (Alcock, 1893), whose biological function is not clear.

Discussion

The COI genetic distances (<0.003) fall in the range of intraspecific distance suggested by Ward, Holmes & O’Hara (2008) for Asteroidea, which confirmed that specimens of A. patricki sp. nov. are indeed the same species. Based on the current dataset, the new species is genetically closer to Brisinga species (0.123–0.159) rather than to Hymenodiscus Perrier, 1884 species (0.194–0.203). The former distance barely falls out of the range of congeneric divergence of Asteroidea, whereas the latter is an undoubted intergeneric distance (Ward, Holmes & O’Hara, 2008). Similar results were also suggested by the phylogenetic analyses. In the Brisingidae clade, A. patricki sp. nov. appears to be intermediate between the Brisinga clade and Hymenodiscus spp., forming a sister clade with Brisinga spp. and Freyellaster fecundus (Fig. 5). The molecular evidence is to some degree inconsistent with the morphological taxonomy. Based on morphological characteristics, Hymenodiscus and Astrolirus could be differentiated from other Brisingidae genera in having the paired marginal plates between the first adambulacral plates. Hymenodiscus further differs from Astrolirus in having bare integument between costae. Previous cladistic analysis on Brisingida phylogeny also suggest that the two genera should cluster as a derived clade (Mah, 1998a; Mah, 1998b). The reason for such a discrepancy between molecular and morphological evidence is not clear yet. In addition, several nodes in the ML tree were support with low bootstrap value (Fig. 5), possibly owing to data deficiency. Genetic data from other Brisingidae species and genera should be supplemented in the future for a comprehensive phylogenetic analysis.

Conclusions

The new species Astrolirus patricki sp. nov. reported in this study represents the second known species in the genus, which is distinguished from its congener by the form and organization of costae and intercostal plates as well as numbers of spines and arms. Morphological description and molecular data delimitate the new species and provide reference for future taxonomic and phylogenetic study of related species. The current phylogenetic analysis on order Brisingida indicates an intermediate position of A. patricki sp. nov. between Brisinga and Hymenodiscus, but more samples and multi-gene analysis are needed in the future to clarify the actual systematic and phylogenetic relationships among these genera.

Supplemental Information

GenBank accession numbers of taxa used in phylogenetic analyses

DOI: 10.7717/peerj.9071/supp-1