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All the reviewer's suggestions were properly considered by the authors.
[# PeerJ Staff Note - this decision was reviewed and approved by Vladimir Uversky, a PeerJ Section Editor covering this Section #]
Please, address all the minor and major points raised by the three reviewers.
[# PeerJ Staff Note: It is PeerJ policy that additional references suggested during the peer-review process should only be included if the authors are in agreement that they are relevant and useful #]
In the introduction section the nomenclature for chromosomal positions is not correct. Please Change the description for OTX1 and OTX2 into 2p15 and 14q22, respectively.
In the result section the text would gain understandability if each chapter ends with a short summary.
The paper submitted by Bistoletti and coworkers deals with the role of homeodomain proteins OTX1 and OTX2 in DNBS-induced colitis in rats. The study is well performed and written.
The manuscript is well structured. The findings are interesting, however, it is missing important information related to the modulations observed and mechanisms.
- Abstract: Ok
- Introduction: The introduction could be improved. Please include more previous date about OTX1 and OTX2
- methods: OK
- Results: See 2. experimental design.
- Discussion: There are a lot of introuction in the discussion. In the discussion section you must compare your date with other manuscripts from other groups.
- Conclusion: Please include only the conclusion, exclude speculation.
About the experiments;
1) It is missing bright field microscopy in all microscope images. Please include in
- Figure 1
- Figure 2
- Figure 5
- Figure 6
- Figure 7
- Figure 8
2) For OTX1, the staining is present in all cells and usually the co-locacization is always observed. Is it right?
3) The image of western-blot for OTX1 and OTX2 is not clear as described in the figures 9B and D.
4) The citokines must be quantified by elisa.
5) Please, include IL-17, IL-23 and TGF-beta. Colite is associated to TH17 response and Th17 was not evaluated.
6) Please correct, in mRNA you detect pro-IL1beta.
The authors used only one methodology to prove the finding (microscope). If used other techniques as Flow-cytometer will increase the strength of the manuscript.
The manuscript is interesting, however, it is missing mechanisms.
1) What is the function of the increased of OTX1 and OTX2 after DNBS treatment?
2) What is the function of OTX1 superimposable with inducible nitric oxide synthase (iNos)? and nNOS?
3) Is it the increase of OTX1 and OTX2 associated to increase of citokines? Which?
4) Is the increase of OTX associated to microbiota? It will be interesting to treat mice with antibiotics.
5) If you prime cells in vitro with LPS, is it possible to induce the same mechanism?
The paper is interesting
I suggest the authors to add in the introduction other drugs used for intestinal inflammatios, see these papers:
Jo A, Yoo HJ, Lee M. Robustaflavone Isolated from Nandina domestica Using
Bioactivity-Guided Fractionation Downregulates Inflammatory Mediators. Molecules.
2019 May 8;24(9).
Szebeni GJ, Nagy LI, Berkó A, Hoffmann A, Fehér LZ, Bagyánszki M, Kari B,
Balog JA, Hackler L Jr, Kanizsai I, Pósa A, Varga C, Puskás LG. The
Anti-Inflammatory Role of Mannich Curcuminoids; Special Focus on Colitis.
Molecules. 2019 Apr 19;24(8).
Pagano E, Romano B, Iannotti FA, Parisi OA, D'Armiento M, Pignatiello S,
Coretti L, Lucafò M, Venneri T, Stocco G, Lembo F, Orlando P, Capasso R, Di Marzo
V, Izzo AA, Borrelli F. The non-euphoric phytocannabinoid cannabidivarin
counteracts intestinal inflammation in mice and cytokine expression in biopsies
from UC pediatric patients. Pharmacol Res. 2019 Nov;149:104464.
Pagano E, Capasso R, Piscitelli F, Romano B, Parisi OA, Finizio S, Lauritano A, Marzo VD, Izzo AA, Borrelli F. An Orally Active Cannabis Extract with High Content in Cannabidiol attenuates Chemically-induced Intestinal Inflammation and Hypermotility in the Mouse. Front Pharmacol. 2016 Oct 4;7:341.
Capasso R, Orlando P, Pagano E, Aveta T, Buono L, Borrelli F, Di Marzo V, Izzo AA. Palmitoylethanolamide normalizes intestinal motility in a model of post-inflammatory accelerated transit: involvement of CB₁ receptors and TRPV1 channels. Br J Pharmacol. 2014 Sep;171(17):4026-37.
The manuscript would benefit from inclusion of introducing/bridging sentences between the individual parts of the "Results" that explain the logical order and rationale for the experiments
In the Discussion, the Authors should highlight the possible clinical significance of their findings
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