TY - JOUR UR - https://doi.org/10.7717/peerj.6588 DO - 10.7717/peerj.6588 TI - Proteomics alterations in chicken jejunum caused by 24 h fasting AU - Simon,Ádám AU - Gulyás,Gabriella AU - Mészár,Zoltán AU - Bhide,Mangesh AU - Oláh,János AU - Bai,Péter AU - Csősz,Éva AU - Jávor,András AU - Komlósi,István AU - Remenyik,Judit AU - Czeglédi,Levente A2 - Bentley,George DA - 2019/03/26 PY - 2019 KW - 2D-DIGE KW - Chicken KW - Expression KW - Fasting KW - qPCR KW - Small intestine KW - Morphometry KW - Villus KW - mRNA KW - Protein AB - The small intestine is the longest part of the chicken (Gallus gallus) gastrointestinal system that is specialized for nutrient absorption. It is known that decrease in intestinal villi area or height in early age can cause a reduction in essential nutrient intake, which may lead to delayed growth and consequently poorer performance of broiler chickens. The small intestinal absorptive surface is known to be affected by various factors, among others things the nutritional state. In our experiment, we aimed to investigate the possible protein expression alterations that lie behind the villus area and height decrease caused by feed deprivation. A total of 24 chickens were divided into three groups, namely ad libitum fed, fasted for 24 h, fasted for 24 h then refed for 2 h. The morphometric parameters were also measured in the duodenum, jejunum and ileum tissue sections using image analysis. Differential proteome analyses from jejunum samples were performed using two-dimensional difference gel electrophoresis followed by tryptic digestion and protein identification by matrix-assisted laser desorption/ionization mass spectrometry. Overall 541 protein spots were detected after 2D. Among them, eleven showed 1.5-fold or higher significant difference in expression and were successfully identified. In response to 24 h fasting, the expression of nine proteins was higher and that of two proteins was lower compared to the ad libitum fed group. The functions of the differentially expressed proteins indicate that the 24 h fasting mainly affects the expression of structural proteins, and proteins involved in lipid transport, general stress response, and intestinal defense. VL - 7 SP - e6588 T2 - PeerJ JO - PeerJ J2 - PeerJ SN - 2167-8359 ER -