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Melissa Carew
PeerJ Author & Reviewer
170 Points

Contributions by role

Author 135
Reviewer 35

Contributions by subject area

Biodiversity
Entomology
Genetics
Freshwater Biology
Ecology
Zoology

Melissa E Carew

PeerJ Author & Reviewer

Summary

My main interests are understanding species diversity using DNA barcodes and developing DNA-based tools for environmental monitoring, especially in freshwater ecosystems. In the past, my research has largely focused on using DNA approaches to understand life histories, population structure and for species identification of various invertebrate (and some vertebrate) groups. During my PhD, I developed DNA approaches for species identification of Chironomidae (Diptera), well known as a bioindicator group in freshwater environments. I also investigated the bioidicator potential in particular chironomid species by examining their field-based responses to pollution. More recently, my work has broadened to include identifying many species of freshwater macroinvertebrates using DNA barcoding. I am currently developing next generation sequencing (NGS) approaches or 'metabarcoding' to facilitate fast, cost-effective identification of freshwater macroinvertebrates collected from streams/rivers in urban and surrounding areas. I am also using metabarcoding of macroinvertebrate field samples to determine species-level environmental responses in order to provide more sensitive and diagnostic monitoring of freshwater ecosystems for water managers.

Biodiversity Entomology Freshwater Biology Genetics

Past or current institution affiliations

University of Melbourne

Work details

Research Fellow

The University of Melbourne
BioScience
Current Projects: Improved biomonitoring of urban freshwater ecosystems using DNA barcodes -ARC Linkage project with Melbourne Water corporation (LP150100876) Summary: Next generation sequencing (NGS) can transform monitoring of aquatic ecosystems by identifying many species in multiple biological samples. This project uses new NGS technology to identify macroinvertebrate species as biological indicators in urban freshwater ecosystems to provide more rapid, sensitive and cost-effective monitoring compared to traditional methods. We do this by taking DNA barcodes created to identify species and use these to detect species in samples processed with NGS. Previously collected samples are used to explore species diversity and detect indicator species. This project offers a much needed update of freshwater monitoring in urban areas by offering rapid diagnostic detection of environmental change at reduced cost.

PeerJ Contributions

  • Articles 1
  • Reviewed 1
June 13, 2018
Can non-destructive DNA extraction of bulk invertebrate samples be used for metabarcoding?
Melissa E. Carew, Rhys A. Coleman, Ary A. Hoffmann
https://doi.org/10.7717/peerj.4980 PubMed 29915700

Signed reviews submitted for articles published in PeerJ Note that some articles may not have the review itself made public unless authors have made them open as well.

December 5, 2018
Simultaneous preservation of the DNA quality, the community composition and the density of freshwater oligochaetes for the development of genetically based biological indices
Régis Vivien, Inge Werner, Benoit J.D. Ferrari
https://doi.org/10.7717/peerj.6050 PubMed 30568859