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Primary producers may ameliorate impacts of daytime CO2 addition in a coastal marine ecosystem

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RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
RT @thePeerJ: Primary producers may ameliorate impacts of daytime CO2 addition in a coastal marine ecosystem https://t.co/2EES9W8f3O https:…
Primary producers may ameliorate impacts of daytime CO2 addition in a coastal marine ecosystem https://t.co/2EES9W8f3O https://t.co/qfUCkJTuXu
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
100 days ago
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
RT @BrackenLab: #Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbige…
#Seaweeds mitigate impacts of experimental CO2 additions in coastal habitats - new paper with my great co-authors @NSilbiger @Bernatchez_G @CascadeJBS #OceanAcidification @UCIBioSci @UCI_OCEANS @csunorthridge https://t.co/4lhHM1v1ph https://t.co/k9XsIldfOs
Our article was published today in @thePeerJ https://t.co/VpW3NrSkfP #Ecology #MarineBiology #ClimateChangeBiology #Biogeochemistry

Introduction

Increased concentrations of CO2 in the atmosphere have already altered oceanic carbonate chemistry, resulting in a decline in overall pH by ∼0.1 units since the year 1800 (Sabine et al., 2004; Orr et al., 2005). As atmospheric CO2 continues to increase, pH changes are predicted to accelerate, leading to an additional decline of 0.07 (RCP2.6, “high mitigation” scenario) to 0.33 (RCP8.5, “business-as-usual” scenario) pH units over the next 80 years (Bopp et al., 2013). Ocean acidification is predicted to dramatically affect marine ecosystems (Harley et al., 2006; Doney et al., 2009). Important resources (e.g., calcifying plankton) and foundation species (e.g., corals, mollusks) will have difficulty maintaining their calcium carbonate skeletons (e.g., Orr et al., 2005; Pfister et al., 2016), leading to declines in growth, abundance, and survival (Kroeker et al., 2013). Predicting the impacts of ocean acidification on marine communities and ecosystems is therefore critical. However, these predictions are difficult in coastal ecosystems, where carbonate chemistry is already extremely dynamic (e.g., Wootton, Pfister & Forester, 2008; Thomsen et al., 2010; Hofmann et al., 2011; Guadayol et al., 2014; Chan et al., 2017; Koweek et al., 2017; Silbiger & Sorte, 2018).

The range in pH values recorded in many coastal systems is greater than the pH decline predicted by the year 2100 under the RCP8.5 “business as usual” scenario (Bopp et al., 2013; Hofmann et al., 2011; Sorte & Bracken, 2015; Silbiger & Sorte, 2018). For example, coastal upwelling transports depth-derived, low-pH waters to the coast, resulting in some of the lowest pH values ever recorded in the surface ocean (Feely et al., 2008; Chan et al., 2017). Additionally, fluxes of dissolved inorganic carbon, associated with processes such as photosynthesis and respiration, are substantially higher in coastal systems than in the open ocean, leading to the suggestion that ocean acidification is an “open-ocean syndrome” (Duarte et al., 2013). Predicting the impacts of ocean acidification in coastal habitats is therefore complicated by the biogeochemical processes mediated by the resident biota, which drive local-scale pH variability (Wootton, Pfister & Forester, 2008; DeCarlo et al., 2017; Silbiger & Sorte, 2018). All organisms respire, increasing CO2 concentrations and reducing pH (Cai et al., 2011; Kwiatkowski et al., 2016). Primary producers, however, also photosynthesize, drawing down CO2 levels and increasing pH (Duarte et al., 2013; Hendriks et al., 2014), and natural pH variability can be exceptionally high in vegetated marine habitats such as seagrass meadows and kelp forests (Hendriks et al., 2014; Kapsenberg & Hofmann, 2016; Koweek et al., 2017). Both mean and maximum pH values increase with the photosynthetic capacity of primary producers (Hendriks et al., 2014), highlighting a potential role for submerged aquatic vegetation to reduce some of the impacts of ocean acidification in coastal ecosystems (Delille et al., 2000; Semesi, Beer & Björk, 2009; Silbiger & Sorte, 2018).

Effective predictions of ocean acidification impacts in coastal systems must consider the biogeochemical, oceanographic, and hydrodynamic context—including ecosystem metabolism—of these ecosystems (e.g., Hofmann et al., 2011; Koweek et al., 2017). A variety of approaches have been taken toward accomplishing this goal, including in situ measurements of carbonate chemistry parameters in multiple coastal ecosystems (e.g., Hendriks et al., 2014; Silbiger et al., 2014; Kwiatkowski et al., 2016; Silbiger & Sorte, 2018), characterization of natural communities associated with volcanic CO2 seeps (Fabricius et al., 2011; Kroeker et al., 2011), and experimental additions of CO2 in the field (Kline et al., 2012; Campbell & Fourqurean, 2013; Sorte & Bracken, 2015; Brown, Therriault & Harley, 2016; Cox et al., 2016). Field manipulations are an especially promising approach, but adding CO2 to a dynamic coastal environment is difficult due to short water-residence times and the expense and logistical difficulty of adding regulated CO2 gas (Gattuso et al., 2014). Here, we leverage experience from previous work developing cost-effective yeast reactors to generate CO2 in situ in tide pools on intertidal rocky reefs (Sorte & Bracken, 2015) to evaluate how the effects of CO2 additions on carbonate parameters are affected by the resident biota.

We used short-term manipulations of natural tide pool habitats on the coast of northern California, USA, to evaluate the effects of increased CO2 on pH and pCO2 in the context of highly variable community compositions (Bracken et al., 2008) and carbonate dynamics (Kwiatkowski et al., 2016; Silbiger & Sorte, 2018). Our experiments were conducted during daylight hours at low tide on two concurrent days in the spring of 2016, when pools were isolated by the receding tide in the morning and remained isolated for ∼8 h. We hypothesized that impacts of CO2 addition on seawater carbonate dynamics would be mediated by primary producers in the tide pools. We predicted that pCO2 would decline and pH would increase over the course of our experiment due to photosynthetic fixation of CO2 (Hendriks et al., 2014; Kwiatkowski et al., 2016; Silbiger & Sorte, 2018) and that the effects of CO2 addition on pH and pCO2 would diminish with increasing percent cover of primary producers in the pools (Hendriks et al., 2014).

Materials and Methods

Study location and characteristics

We conducted a CO2 addition experiment on a rocky intertidal shoreline on the northern side of Horseshoe Cove in the Bodega Marine Reserve (BMR), Sonoma County, California, USA (NAD83 38.31672, −123.0711). This work was conducted with the approval of the California Department of Fish and Wildlife (Scientific Collecting Permit number SCP-13405). Our work in the BMR was performed under Research Application # 32752 to the University of California Natural Reserves System to conduct measurements of carbonate chemistry in tide pools. At the site, we marked and surveyed 10 tide pools in the mid-intertidal zone. For each pool, we determined tidal elevation (mean = 1.54 ± 0.05 [s.e.] m above mean lower-low water; range = 1.22 to 1.73 m), volume (mean = 22.3 ± 5.0 L; range = 6.7 to 47.3 L), and bottom surface area (mean = 0.35 ± 0.08 m2; range = 0.16 to 1.00 m2). Tidal elevations were determined using a self-leveling rotary laser level (CST/berger, Watseka, Illinois, USA), with reference to published tidal predictions for Bodega Harbor Entrance (NOAA, 2016). Pool volumes were determined by adding 2 ml of non-toxic blue dye (McCormick, Sparks, Maryland, USA) to each pool. Water samples were read at 640 nm on a benchtop spectrophotometer (UV-1800, Shimadzu, Carlsbad, California, USA) and compared to a standard curve relating absorbance to an equivalent amount of dye added to known volumes of seawater (Pfister, 1995). The abundances of primary producers and sessile invertebrates in each tide pool were determined by spreading a flexible mesh grid (10 cm × 10 cm mesh; Foulweather Trawl Supply, Newport, Oregon, USA) over the bottom of each pool and measuring the cover of macrophytes (seaweeds and surfgrass) and sessile invertebrates (barnacles, mussels, sea anemones, and sponges) and the total surface area of each tide pool (Bracken & Nielsen, 2004) (see Table S1). Mobile invertebrates (particularly turban snails) were also counted in each pool. Tide pool volumes, primary producer abundances, and surface areas were measured immediately after our experiment to avoid disturbing the pools prior to water sampling.

Experimental design and sampling

These pools included some of the same tide pools used in other studies of carbonate chemistry under ambient conditions: Kwiatkowski et al. (2016) measured calcification during the spring of 2014 and 2015, and Silbiger & Sorte (2018) quantified bio-physical feedbacks during the summer of 2016. Our CO2 addition experiments were conducted on two consecutive days: 31 March and 01 April 2016. On those days, the receding tide isolated pools in the morning (08:30–09:30 on day 1, 09:00–10:00 on day 2), and they remained isolated for approximately 7.5 hr. Pools were randomly assigned to experimental treatments: control or +CO2, with n = 5 pools assigned to each treatment. There were no differences in tidal elevation (t = 1.4, df = 8, p = 0.210), volume (t = 0.6, df = 8, p = 0.570), or surface area (t = 0.8, df = 8, p = 0.453) between control and +CO2 pools. Treatments were switched between day 1 and day 2, so that pools assigned to +CO2 treatments on day 1 were assigned to control treatments on day 2, and vice versa. This switching allowed us to assess the effect of CO2 addition on carbonate chemistry within individual pools by quantifying the difference between day 1 and day 2. We assume that because CO2 additions were within the natural variability of the system, there were minimal carryover effects of day 1 CO2 additions to day 2 control tide pools. Average air temperature (day 1: 11.2 ± 0.3 °C; day 2: 11.8 ± 0.4 °C), wind speed (day 1: 14.7 ± 1.3 km hr−1; day 2: 16.8 ± 0.5 km hr−1), and photosynthetically active radiation (day 1: 871±188 µmol m−2 s−1; day 2: 1,058 ± 173 µmol m−2 s−1) values, measured at the Bodega Ocean Observing Node weather station adjacent to our study site, did not differ substantially between day 1 and day 2.

CO2 was delivered to +CO2 pools using yeast reactors consisting of watertight plastic boxes (Drybox 2500, OtterBox, Fort Collins, Colorado, USA) containing 500 mL of warm water, 2 g of NaHCO3 (to buffer the internal pH of the reactor), and 0.7 g of baker’s yeast, an amount calculated to generate pH levels and pCO2 concentrations that were within the predicted range of 21st-century ocean acidification scenarios (Bopp et al., 2013). Tubing from each reactor led to an air stone anchored within each +CO2 tide pool. CO2 is the most abundant gas produced by baker’s yeast, representing ∼99.2% of headspace volume (Daoud & Searle, 1990), though additional volatile organic compounds such as ethanol, 2-methylpropanol, and ethyl acetate are present in low concentrations (Smallegange et al., 2010).

We calibrated the yeast reactors and determined the amount of yeast to use in our experiment by running a series of trials in buckets across a range of temperatures and yeast amounts. Buckets (n = 24) contained 12 L of saltwater at typical ocean concentrations (Instant Ocean® Sea Salt, Blacksburg, Virginia, USA). Temperature was controlled by placing buckets in growth chambers (Percival Scientific, Perry, Iowa, USA) set to 4°, 10°, 20°, or 30 °C. We measured pH (total scale) in each bucket every hr for 6 hr and monitored temperature using TidbiT® v2 datalogers (Onset Computer Corporation, Bourne, Massachusetts, USA). These trials also represent controls, allowing us to evaluate the effects of yeast and temperature on tide pool pH in the absence of organisms.

Seawater chemistry and other environmental conditions were measured throughout the low tide period on each day. Water samples were collected from pools every 1.5 hr for 6 hr, beginning as soon as each pool was isolated by the receding tide. However, due to nonlinearity in the carbonate parameters across time—potentially due to carbon limitation as pCO2 declined over time (Maberly, 1990)—we calculated rates of change in pH, pCO2, net community production, and net ecosystem calcification using only the initial and final samples for each pool, which represented the overall net change over the sampling period. Temperature, dissolved oxygen and conductivity were simultaneously measured in each pool using sensors (ProODO and Pro30, YSI, Yellow Springs, Ohio, USA). Samples were collected by pumping water (400 mL) into a plastic Erlenmeyer flask using a separate piece of tubing anchored in each pool to minimize disturbance and off-gassing. Anchoring the tubing in each pool also ensured that our samples were collected from the same location every time and that they were not collected adjacent to the airstones delivering CO2 to +CO2 pools. Each water sample was immediately divided into subsamples for analyses of pH, total alkalinity, and dissolved inorganic nutrients. Prior to sample collection, all containers were washed in 10% HCl, rinsed 3× with DI water, and rinsed 3× with ocean water. We measured pH by measuring voltage (mV) and temperature (°C) of a 50 mL subsample immediately after collection using a multiparameter pH meter (Orion Star, Thermo Fisher Scientific, Waltham, Massachusetts, USA) with a ROSS Ultra glass electrode (Thermo Scientific, USA; accuracy ± 0.2 mV, resolution ± 0.1, drift <0.005 pH units per day) and a traceable digital thermometer (5-077-8, accuracy =0.05°C, resolution = 0.001 °C; Control Company, Friendswood, TX, USA). pH (total scale) was calculated using a multipoint calibration to a Tris standard (Marine Physical Laboratory, Scripps Institution of Oceanography, La Jolla, California, USA), as described in SOP 6a (Dickson, Sabine & Christian, 2007). Subsamples for determination of total alkalinity were fixed with 100 µL of 50% saturated HgCl2 and stored in 250 mL brown HDPE bottles. Subsamples for dissolved inorganic nutrients (NO 3 , NO 2 , NH 4 + , and PO 4 3 ) were filtered (GF/F, Whatman, Maidstone, UK) into 50 ml centrifuge tubes and frozen at −20 °C prior to analyses.

Sample processing

Subsamples for total alkalinity were analyzed using open-cell titrations (T50, Mettler-Toledo AG, Schwerzenbach, Switzerland), as described in SOP 3b (Dickson, Sabine & Christian, 2007). A certified reference material (Marine Physical Laboratory, Scripps Institution of Oceanography, La Jolla, California, USA) was run daily. Our measurements of the reference material never deviated more than ±0.4% from the certified value, and alkalinity calculations were corrected for these deviations. NO 3 + NO 2 and PO 4 3 concentrations (µmol L−1) were measured on a QuickChem 8500 Series Analyzer (Lachat Instruments, Loveland, Colorado, USA), and NH 4 + concentrations (µmol L−1) were measured using the phenolhypochlorite method (Solórzano, 1969) on a UV-1800 benchtop spectrophotometer (Shimadzu, Carlsbad, California, USA). In situ pH values and other carbonate parameters were calculated using the seacarb package in R v. 3.2.2 (R Foundation for Statistical Computing, Vienna, Austria) (Gattuso et al., 2017) (Table S2). We note that error propagation for calculating Ωarag based on pH and TA is ∼3.6% (Riebesell et al., 2011).

Calculation of net community production and net ecosystem calcification in control tide pools

Differences in dissolved inorganic carbon (DIC) were used to calculate net community production (NCP) rates (mmol C m−2 hr−1), as described in Gattuso, Frankignoulle & Smith (1999): NCP = Δ DIC ρ V SA t NEC F CO 2 where ΔDIC is the difference in salinity-normalized DIC between the first and last time points (in this case 0 and 6 hr; mmol kg−1), ρ is the density of seawater (1,023 kg m−3), V is the volume of water in the tide pool at each time point, SA is the bottom surface area of each pool (m2), and t is the time interval between sampling points (6 hr).

NEC is the rate of net ecosystem calcification (mmol CaCO3 m−2 hr−1), which was calculated as follows: NEC = Δ TA ρ V 2 SA t where, in addition to variables defined above, ΔTA/2 is the difference in total alkalinity (TA, mmol kg−1) between the time points. TA was normalized to a constant salinity and corrected for dissolved inorganic nitrogen and phosphorus to account for their small contributions to the acid–base system (Wolf-Gladrow et al., 2007), including the potential for primary producers to change alkalinity via nutrient uptake (Brewer & Goldman, 1976; Stepien, Pfister & Wootton, 2016). One mole of CaCO3 is formed per two moles of TA, hence the divisor of 2.

Finally, FCO2, the air-sea flux of CO2 (mmol m−2 hr−1), was calculated as follows: F CO 2 = k s CO 2 water CO 2 air where k is the gas transfer velocity (m hr−1), and s is the solubility of CO2 in seawater, which was calculated based on in situ measurements of temperature and salinity (Weiss, 1974). The concentration of CO2 in air was assumed to be 400 µatm (Tans & Keeling, 2017). The transfer velocity of CO2 was based on wind velocities measured at the Bodega Ocean Observing Node weather station located 100 m from our study location. Calculated FCO2 values (mmol kg−1 hr−1) were converted to mmol CO2 m−2 hr−1 based on the volume of the tide pool, the density of seawater, and the bottom surface area. All data for NCP, NEC, and FCO2 are provided in the electronic supplementary materials (Table S3).

Statistical analyses

Changes in pH in buckets were evaluated as a function of temperature (C) and yeast (g) using multiple linear regression (PROC GLM) in SAS v. 9.4 (SAS Institute, Cary, North Carolina, USA). Changes in pH and pCO2 in field tide pools over time (i.e., pH units hr−1 or µatm hr−1) were calculated by subtracting the initial value (0 hr) from the final value (6 hr) and dividing by the elapsed time. The difference between +CO2 and control pools was evaluated for each individual pool (the experimental unit in all analyses). We quantified the effect of macrophytes (seaweeds and surfgrass) on carbonate parameters by calculating the cover of non-calcifying macrophytes in each tide pool. We excluded calcifying species because of low photosynthetic biomass relative to non-calcifying species from this location (Bracken & Williams, 2013). On average, less than 5% of algal cover in the pools was composed of calcifying seaweeds. We also quantified the effect of invertebrates by estimating total invertebrate cover based on our surveys. For mobile invertebrates (mostly turban snails), we estimated cover from count data based on the number of individuals of each species in a 10 cm × 10 cm quadrat (=0.01 m2). We then evaluated relationships between macrophyte and invertebrate abundances and net community production (NCP) using linear regression (PROC GLM) in SAS. To account for the effects of primary producers on pH and pCO2, we used linear regression to evaluate the difference between control and +CO2 tide pools as a function of the abundance of macrophytes in the pools. We calculated these differences by subtracting the rates of change in pH (total scale hr−1) or pCO2 (µatm hr−1) in each pool under ambient CO2 conditions from the rates when CO2 was added to those same pools. Over time, pH increased and pCO2 decreased due to photosynthesis. Thus, a negative effect of CO2 addition on pH indicated that CO2 addition reduced pH in +CO2 pools relative to control pools. Conversely, a positive effect of CO2 addition on pCO2 indicated that CO2 addition enhanced pCO2 in +CO2 pools relative to control pools. We assessed how pH, pCO2, net ecosystem calcification (NEC), and O2 responded to changes in NCP under ambient CO2 conditions using linear regression (PROC GLM in SAS). Similarly, we evaluated whether NEC was related to cover of calcifiers (mussels, turban snails, and coralline algae) using linear regression. Prior to running linear regressions, we verified that data met the assumptions of normality and homogeneity of variances.

Results

Biological processes resulted in substantial changes in both pH and pCO2 in tide pools over the course of a single low tide: pH increased by ∼0.4 units and pCO2 decreased by ∼233 µatm in both control and +CO2 pools (Fig. 1; Table S4). The effects of our experimental CO2 additions on pH and pCO2 were masked by the dominant effects of primary producers on carbonate chemistry; there was no apparent difference in either the pH (t = 0.6, df = 9, P = 0.591; Fig. 1A) or the pCO2 (t = 0.3, df = 9, P = 0.777; Fig. 1B) of control versus +CO2 pools. Physical parameters –such as changes in temperature (R2 = 0.17, F1,8 = 1.6, P = 0.238) and light availability (R2 <0.01, F1,8 <0.1, P = 0.996)—had minimal effects, by themselves, on changes in tide pool pH.

Tide pool pH and pCO2 values measured over time.

Figure 1: Tide pool pH and pCO2 values measured over time.

Over 6 hr following isolation of pools by the receding tide, (A) pH increased by an average of 0.4 units (total scale) and (B) pCO2 declined by an average of ∼233 µatm. However, there were no differences in pH (P = 0.591) or pCO2 (P = 0.777) between control pools (with no CO2 added; red circles) and +CO2 pools (blue triangles). Values are means ±s.e.

Effects of CO2 additions on pH and pCO2 only became apparent after accounting for primary producer abundances in the tide pools (Fig. 2). As the abundance of macrophytes (seaweeds and surfgrass) increased, the negative effect of CO2 addition on pH was ameliorated (R2 = 0.45; F1,8 = 6.6, P = 0.033; Fig. 2A), indicating that macrophytes limited the reduction in pH associated with CO2 addition. The effect of CO2 addition on pH in the absence of non-calcifying macrophytes is represented by the y-intercept of this relationship (Fig. 2A), which indicates a reduction in pH of 0.07 (±0.03 s.e.) units hr−1 (t = 2.1, df = 9, P = 0.062). We compared this value to the change in pH predicted by our bucket calibration trials. In the absence of organisms, pH declined more rapidly as temperature (F1,21 = 20.1, P < 0.001) and yeast amount (F1,21 = 44.2, P < 0.001) increased. We used the parameter estimates from the multiple linear regression, the amount of yeast (0.7 g), and the ambient tide pool water temperature (17.7 °C) to predict the change in pH in the absence of organisms. We predicted a reduction in pH of 0.06 (±0.03 s.e.) units hr−1, which is similar to the observed rate in the absence of macrophytes (t = 0.2, df = 32, P = 0.845). Correspondingly, as macrophyte cover increased in tide pools, the effect of CO2 addition on pCO2 was reduced (R2 = 0.42; F1,8 = 6.0, P = 0.040; Fig. 2B). The y-intercept of this relationship (Fig. 2B) represents the effect of CO2 addition on pCO2 in the absence of non-calcifying macrophytes and indicates an increase of 81.22 (±33.80 s.e) µatm hr−1 (t = 2.4, df = 9, P = 0.041).

Effects of CO2 addition on rates of change in pH (total scale hr−1) and pCO2 (µatm hr−1) in tide pools were reduced as macrophyte cover increased.

Figure 2: Effects of CO2 addition on rates of change in pH (total scale hr−1) and pCO2 (µatm hr−1) in tide pools were reduced as macrophyte cover increased.

The effect of CO2 addition was defined for each pool as the rate of change when CO2 was added minus the rate when CO2 was at ambient levels. As macrophyte abundance in the pools increased, (A) the effect of CO2 addition on pH became more positive (R2 = 0.45, P = 0.033) and (B) the effect of CO2 addition on pCO2 became more negative (R2 = 0.43, P = 0.040). Rates of change in pH and pCO2 in the absence of macrophytes are indicated by the y-intercept of the regression lines (pH:−0.07 ± 0.03 s.e. total scale hr−1; pCO2: +81.22 ± 33.80 µatm hr−1). Regression lines are means ±95% c.i.

The effects of macrophytes on pH and pCO2 emerged because increases in macrophyte abundance were associated with increases in net community production (NCP, mmol C m−2 hr−1; Fig. 3A). This relationship held for all non-calcifying macrophytes considered together (R2 = 0.47; F1,8 = 7.1, P = 0.029) and was especially apparent for the most abundant macrophyte in the tide pools, the red seaweed Prionitis sternbergii (C. Agardh) J. Agardh (R2 = 0.68; F1,8 = 16.8.1, P = 0.004). Non-calcifying macrophytes, including the seaweeds Gelidium coulteri Harvey, Hildenbrandia rubra Sommerfelt (Meneghini), Mastocarpus papillatus (C. Agardh) Kützing (both upright and Petrocelis forms), Mazzaella splendens (Setchell & N. L. Gardner) Fredericq, and P. sternbergii and the surfgrass Phyllospadix torreyii S. Watson, were abundant in the pools, collectively composing 56% of cover on the benthos (Fig. 3B). However, NCP was not related to cover of any macrophyte species other than Prionitis (e.g., Hildenbrandia [R2 = 0.18; F1,8 = 1.8, P = 0.216], Phyllospadix [R2 = 0.0.1; F1,8 <0.1, P = 0.846]). NCP was also unrelated to abundances of invertebrates (primarily mussels [Mytilus californianus T. A. Conrad], sea anemones [Anthopleura spp.], and turban snails [Tegula funebralis A. Adams]) in the tide pools (R2 = 0.09; F1,8 = 0.8, P = 0.409). Net ecosystem calcification (NEC) was unrelated to abundances of calcifying invertebrates in the tide pools, including Mytilus (R2 = 0.03; F1,8 = 0.2, P = 0.653), Tegula (R2 = 0.03; F1,8 = 0.2, P = 0.654), and coralline algae (R2 <0.01; F1,8 = 0.1, P = 0.788).

Effects of macrophytes on net community production (NCP, mmol C m−2 hr−1).

Figure 3: Effects of macrophytes on net community production (NCP, mmol C m−2 hr−1).

(A) Increases in macrophyte cover were associated with increases in NCP for both the most abundant macrophyte, the red alga Prionitis sternbergii (red circles, solid trendline; R2 = 0.68, P = 0.004), and all macrophytes (blue triangles, dashed trendline; R2 = 0.47, P = 0.029). Inset image: P. sternbergii. Image credit: Matthew Bracken. (B) Macrophytes, including seaweeds and the surfgrass Phyllospadix, dominated cover in the pools, collectively occupying the majority of the substratum. Macrophytes in the “Other” category each contributed <5% of cover and included Cladophora, Endocladia, Mazzaella flaccida, and calcifying species. Invertebrates represented ∼15% of total cover.

Rates of change in pH (R2 = 0.63; F1,8 = 13.7, P = 0.006; Fig. 4A), pCO2 (R2 = 0.43; F1,8 = 6.1, P = 0.039; Fig. 4B), and NEC (R2 = 0.67, F1,8 = 16.2, P = 0.004; Fig. 4C) were strongly related to changes in NCP. O2 production in the tide pools was also strongly related to NCP (R2 = 0.65, F1,8 = 14.7, P = 0.005; Fig. 4D), linking macrophyte abundances (Fig. 3A), NCP (measured by evaluating carbonate parameters), and O2 production.

Relationships between net community production (NCP) and other carbonate parameters in tide pools.

Figure 4: Relationships between net community production (NCP) and other carbonate parameters in tide pools.

Increases in NCP (mmol C m−2 hr−1) in control tide pools were associated with (A) increases in pH (total scale hr−1; R2 = 0.62, P = 0.007), (B) declines in pCO2 (µatm hr−1; R2 = 0.47, P = 0.029), (C) increases in net ecosystem calcification (mmol CaCO3 m−2 hr−1; R2 = 0.67, P = 0.004), and (D) increases in O2 concentrations (mg m−2 hr−1; R2 = 0.65, P = 0.005).

Discussion

Primary producers modified the effects of CO2 addition on pH and pCO2 in the tide pools, ameliorating the negative effect of CO2 addition on pH and reducing the effect of CO2 addition on pCO2. In fact, at the highest macrophyte abundances, the effect of CO2 addition on the change in pH was positive, indicating that added CO2 may be ameliorating carbon limitation and enhancing rates of photosynthesis (e.g., Gao et al., 1991)—and thereby amelioration of low pH conditions—during the day. Based on rates of change in pH and pCO2 in the absence of macrophytes, by the time pools were re-submerged by the rising tide, our yeast reactors had, in effect, elevated pCO2 by ∼487 µatm relative to control pools, reducing pH by ∼0.4 units over ∼6 hr. Observed effects of net community production (NCP) on net ecosystem calcification (NEC) highlight the potential for primary producers to not only mediate pH and pCO2, but to also enhance other ecosystem functions during the day. Furthermore, the relationship between O2 production and NCP suggests that CO2 drawdown in the tide pools was due to photosynthesis.

Our results underscore the difficulty of quantifying the effects of realistic levels of CO2 addition on pH and pCO2 in a producer-dominated coastal ecosystem and suggest that macrophytes such as seagrasses and seaweeds have the potential to ameliorate some impacts of ocean acidification in marine systems (Delille et al., 2000; Frieder et al., 2012; Hendriks et al., 2014). As shown in previous research (Delille et al., 2000; Hendriks et al., 2014), including measurements from many of the tide pools included in the current study (Kwiatkowski et al., 2016; Silbiger & Sorte, 2018), photosynthetic uptake of CO2 plays a dominant role in driving carbonate chemistry in coastal marine systems, especially during the daytime (Duarte et al., 2013). For example, we found that NCP was closely coupled to changes in pH, pCO2, and NEC. The fact that macrophyte-mediated enhancement of production also enhanced calcification is particularly important in the context of impacts of ocean acidification on calcifying organisms.

One important caveat, however, is that our experiments were only conducted during daylight hours, when photosynthetic draw-down of CO2 is maximized. Thus, we have no data to address whether macrophytes can ameliorate impacts of CO2 additions at night, when respiration adds CO2 and contributes to dissolution of calcifying species (Kwiatkowski et al., 2016; Silbiger & Sorte, 2018). Can daytime reduction of pCO2 and enhancement of NEC by primary producers help to counterbalance nighttime dissolution? Several lines of evidence suggest that the answer may be yes. First, the enhancement of calcification during daylight hours is substantially greater than dissolution at night in tide pools at our study site, suggesting that producers can counterbalance pH-mediated declines in net calcification (Kwiatkowski et al., 2016). Second, research in seagrass beds indicates that both maximum pH values and mean pH values —averaged across both daytime and nighttime measurements— increase as macrophyte abundance increase, demonstrating a dominant effect of macrophyte abundance on pH overall (Hendriks et al., 2014). Third, photosynthesis, growth, and calcification can be higher under fluctuating pH conditions than under stable conditions (Dufault et al., 2012; Britton et al., 2016; Price et al., 2012). For example, mussels have been shown to shift the timing of shell production to daylight hours when macrophytes ameliorate impacts of elevated CO2 (Wahl et al., 2018). Finally, whereas daytime pH values are strongly related to producer dominance in tide pools, nighttime pH is unrelated to community composition (Silbiger & Sorte, 2018). Higher NCP during the day does not translate to higher community respiration at night, and overall pH values are higher in pools dominated by primary producers.

Tide pools have long served as model systems for evaluating ecological and biogeochemical processes in coastal habitats, as their isolation at low tide allows manipulation and measurement of replicated local ecosystems (e.g., Lubchenco, 1978; Dethier, 1984; Pfister, 1995; Bracken & Nielsen, 2004; Bracken et al., 2008; Sorte & Bracken, 2015). However, they also represent an extreme case, as they are physically isolated from the surrounding ocean during low tide. Although our local-scale CO2 additions only effectively manipulated pH while pools were isolated, there is some evidence, based on research in subtidal kelp beds and seagrass meadows, that marine macrophytes can substantially alter nearshore carbonate chemistry and potentially limit impacts of ocean acidification in more open nearshore systems (Delille et al., 2000; Frieder et al., 2012; Hendriks et al., 2014; Nielsen et al., 2018).

Conclusions

In conclusion, we have shown that primary producers can mediate daytime carbonate chemistry in a coastal ecosystem and that the effects of productivity on pH and pCO2 can mask the effects of short-term CO2 addition during daylight hours. Based on these results, we suggest that primary producers, especially in highly vegetated coastal systems, have the potential to reduce impacts of increasing CO2 concentrations in those systems. However, large, dominant macrophytes, including kelps and seagrasses, that have the potential to ameliorate the impacts of ocean acidification (Frieder et al., 2012; Hendriks et al., 2014), are threatened by overfishing, coastal development, eutrophication, and climate change (Steneck et al., 2002; Orth et al., 2006). Species that have a large capacity to ameliorate impacts of ocean acidification are potential candidates for conservation or restoration. In general, efforts to conserve and restore coastal macrophytes are important for maintaining this capacity in the face of predicted climatic changes (Nielsen et al., 2018).

Supplemental Information

Supplemental Information 1

This file contains the following tables: Table S1. Cover of sessile invertebrates and macrophytes in tide pools. Table S2. Carbonate inputs and parameters from tide pools calculated using seacarb. Table S3. Calculated values for net community production and net ecosystem calcification. Table S4. Ranges in physical attributes and chemical parameters measured in ambient tide pools.

DOI: 10.7717/peerj.4739/supp-1