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The manuscript by Rosales-Calderon et al. is a clearly written solid report which is appropriate for publication in its present form.
The authors demonstrate that protein aggregation combined with non-productive biomass binding is an important feature of cellulase hydrolysis. This finding has practical impacts regarding the use of stabilizers. Their results also suggest that enzyme recycling approaches may not be feasible, since the enzyme released from biomass may be aggregated and inactive.
The authors combine excellent and well-thought-out experiments to clarify models to elucidating the role of beta-glucosidase aggregation on cellulolytic enzyme activities.
The results and concepts gleaned from this study will be of interest to scientists working in the field of biomass conversion to biofuels.
This is a carefully planned and performed study. I highly recommend its publication in PeerJ.
This manuscript deals with an important issue in bioprocessing of biomass and provides new things that will certainly find direct application in various areas of this emerging bioconversion sector.
The experimental design was very well outlined.
the results have been throughly validated.
This manuscript deals with an important issue in bioprocessing of biomass and provides new things that will certainly find direct application in various areas of this emerging bioconversion sector.
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