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Thank you for the English language edits to the revised manuscript, which is now up to the standard of PeerJ and will be informative to readers. I recommend the manuscript for Publication.
Dear Author,
Your article is accepted pending some remaining edits to the English language.
Best regards,
Vijai Kumar Gupta
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The Authors made all the suggested corrections in the manuscript. So, I would like to "ACCEPT" this article.
The effect of agmatine on trichothecene type B and zearalenone production in Fusarium graminearum, F. culmorum and F. poae is suitable for publication.
I found few grammatical / typological errors.
The statistical data analysis is complete
The paper could be strengthened with additional details in the results and discussion, but it is acceptable as it is.
Manuscript accepted for publication.
Dear Author,
Thank you very much for submission of your paper to Peer J. Though you did good work as presented in the paper I agree with the comments made by reviewer 2. So please re-submit your paper after suitable corrections as mentioned by both reviewers.
Best regards,
Vijai Kumar Gupta
no comments, included in comments to authors
no comments, included in comments to authors
no comments, included in comments to authors
In the present paper, the authors want to shed light into the regulatory network of trichothecenes by investigating the importance of specific AA in this process. I have several major comments that I feel are overlooked by the authors, and therefore, the paper is not acceptable for publication.
1. In the abstract, the authors say that other species produce DON and other type B trichothecenes, to my knowledge, F. poae is not a DON producer.
2. It is well known that F. poae produces type A and type B trichothecenes (see paper by Moretti and coworkers). This means that when you only look at a specific subset of toxins, you can never have an idea of the total titer of trichothecenes produced. In this way, you should also verify DAS, NEO,...
3. In the introduction, the authors mention inducers of trichothecenes, still this list is very short, other factors that are known to influence this are not mentioned e.g. light, fungicides, aw value, ...all aspects that have been addressed by other research groups.
4. What is meant with a "moderate toxin producer", this totally not scientific. What do the authors mean with moderate.
5. I do not understand why all the toxins are summed up. especially ZEN which is a structurally totally different toxin. Also the baseline production of trichothecenes (ppm levels) is completely different from that of ZEN (ppb range). So the picture represented is not correct.
6. for me, it is not clear whether the toxin data were calculated taking into account the fungal biomass.
7. statistics: how can you investigate variance and normality by box and Q-Q plot, the authors should use statistical tests. Figures illustrate already that variances are not equal between isolates, so all data should have been investigated using a non-parametric approach
Manuscript entitle "The effect of agmatine on trichothecene type B and zearalenone production in Fusarium graminearum, F. culmorum and F. poae" well written and experimental data presented very nicely.
Well design experiment with data analysis.
no comments
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