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I have confirmed with PeerJ staff that the items mentioned by the reviewer can be addressed during the proofing stage.
**PeerJ Staff Note:** Although the Academic Editor is happy to accept your article as being scientifically sound, a final check of the manuscript shows that it would benefit from further English editing. Therefore, please identify necessary edits and address these while in proof stage.
Please carefully corrected the grammatical, styling, and typos found in your manuscript.
no comment
no comment
References need to be improved in the same format according to the journal’s requirements.
There are duplications in some references. For example,
Li D, Zhang L, Chen M, He X, Li J, and An R. 2018a. Defense Mechanisms of Two Pioneer Submerged Plants during Their Optimal Performance Period in the Bioaccumulation of Lead: A Comparative Study. Int J Environ Res Public Health 15. 10.3390/ijerph15122844
Li D, Zhang L, Chen M, He X, Li J, and An R. 2018b. Defense Mechanisms of Two Pioneer Submerged Plants during Their Optimal Performance Period in the Bioaccumulation of Lead: A Comparative Study. International Journal of Environmental Research & Public Health 15:2844.
Makowski E, Sitko K, Micha S, aneta G, Pogrzeba M, Kalaji HM, and Zielenik-Rusinowska P. 2020. Hormesis in Plants: The Role of Oxidative Stress, Auxins and Photosynthesis in Corn Treated with Cd or Pb. International Journal of Molecular Sciences 21:6.
Malkowski E, Sitko K, Szopinski M, Gieron Z, Pogrzeba M, Kalaji HM, and Zieleznik-Rusinowska P. 2020. Hormesis in Plants: The Role of Oxidative Stress, Auxins and Photosynthesis in Corn Treated with Cd or Pb. Int J Mol Sci 21. 10.3390/ijms21062099
Please carefully corrected the grammar and format in the manuscript.
I am satisfied with the authors response
Experimental design seems good
Findings are valid in lieu of current scenario
I would also recommend using a proficient English Speaker to improve the English quality.
In addition, the statistical analyses should be improved throughout the manuscript.
[# PeerJ Staff Note: The review process has identified that the English language must be improved. PeerJ can provide language editing services - please contact us at copyediting@peerj.com for pricing (be sure to provide your manuscript number and title) #]
[# PeerJ Staff Note: Please ensure that all review comments are addressed in a rebuttal letter and any edits or clarifications mentioned in the letter are also inserted into the revised manuscript where appropriate. It is a common mistake to address reviewer questions in the rebuttal letter but not in the revised manuscript. If a reviewer raised a question then your readers will probably have the same question so you should ensure that the manuscript can stand alone without the rebuttal letter. Directions on how to prepare a rebuttal letter can be found at: https://peerj.com/benefits/academic-rebuttal-letters/ #]
The manuscript deals with the Foliar spraying of IAA enhances the phytostabilization of Pb in naturally tolerant ryegrass by improving the plant growth and limiting the root-to-shoot transfer of Pb. The topic is very interesting. Methodologies followed are standard. The objectives of the study are focused. The authors should improve the aim of the work.
Please clarify the novelty and the justification of your work in the text.
The language should be revised by professional or native language speaker. The manuscript would benefit from the service of a professional language editor.
Please follow the abbreviation rule. Try to write the full name in their first appearance and abbreviation within the bracket, then write the only abbreviation form. Maintain this throughout the manuscript. In some place, you wrote abbreviation in the first appearance without mentioning their full name.
References need to be improved in the same format according to the journal’s requirements.
In the materials and methods section, what is the basis of selection the concentration of 0.1 mmol L-1 IAA.
It would be better to provide data on the physical and chemical properties and element content of the experimental soil.
Please include the data of preliminary experiment of selecting the concentration of IAA.
What is the dimension of pots receiving 50 seedling?
The quality control data should be given.
Recovery means of certified reference materials should be given in the manuscript.
In all Figures enlarge font to be more visible.
Statistical analysis of Figures is wrong.
The Discussion section needs improve in order to become more clear -Discussion is further collection of reports. The reasons for results and relation between data enough has been not emphasized in this section. The manuscript describes Pb concentration，SOD activity , POD activity，effective photochemical quantum yield of PSII , photosynthetic pigments contents, endogenous IAA and biomass, but discussion concerning relation between these effects is very poor.
The discussion section should provide relevant information on IAA interacted with Pb stress.
The use of IAA alone cannot be taken to suggest that exogenous IAA was responsible for the protection against the Pb stress under study in this work. This is also the major weakness in this work.
The article needs to ne improved. The statistical analysis must be changed.
Two variables are present at the work at the same time: the various concentrations of Pb and the application or not of IAA. A one-way ANOVA cannot be applied in this case, analyzing the results as a whole.
A two-way ANOVA should be used comparing both the treatments within each Pb concentration, and comparing each treatment in the several Pb concentrations. The p values of the interactions should also be added to each figure or table.
The results and discussion sections must be rewritten after the new statistical analysis.
No comment.
The results and statistical analysis need to be improved.
I advise authors to redo the statistics of all the results obtained (Two-way ANOVA), and then rewrite the entire results and discussion section already with the support of the new analysis to be carried out. Only then can a more careful analysis be made.
In fact only after redoing the statistical analysis and rewriting the results and discussion will it be possible to rigorously evaluate the article.
Current article describes the role of IAA in phytostabilizing Pb in maize. Topic of research is very important. However, the manuscript requires thorough revision in terms of its grammar. As, in overall draft, poor quality english language is used. I would also recommend using a proficient English Speaker to improve the english quality.
In addition, references used are too old, and require updating. Similarly, results section could be further improved by adding the data of bioconcentration and translocation factors as research theme is very relevant to these factors. Major improvement in the abstract and conclusion section is required. Therefore I reject this manuscript as it is not acceptable in its current form
Expermiental Design used is One way ANOVA, which in my opinion, is not a true one in present case.
Findings are somewhat valid and incomplete to some extent as in my view, phytostabilization or phytoextraction studies require data of bioconcentration and translocation factors and hence, their results should have been added in the results section.
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