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The manuscript is significantly improved by the authors and now can be accepted in its current form.
[# PeerJ Staff Note - this decision was reviewed and approved by Robert Toonen, a PeerJ Section Editor covering this Section #]
The authors proceeded to introduce the corrections suggested by the reviewers, so the manuscript can now be accepted for publication.
Is cooreect
Are valid
Reviewers have raised some concerns and shortcomings in the study. Major revision is suggested, which requires substantial and thorough revision to appreciate the quality of the manuscript. Moreover, thorough English editing is required. Please revise the manuscript taking help from a colleague who is proficient in English and familiar with the subject matter, who can review your manuscript, or contact a professional editing service to review your manuscript. Revise and resubmit accordingly.
[# PeerJ Staff Note: Please ensure that all review and editorial comments are addressed in a response letter and any edits or clarifications mentioned in the letter are also inserted into the revised manuscript where appropriate. #]
[# PeerJ Staff Note: The review process has identified that the English language must be improved. PeerJ can provide language editing services - please contact us at copyediting@peerj.com for pricing (be sure to provide your manuscript number and title) #]
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The manuscript titled "Chemical diversity and antifouling activity of geniculate calcareous algae (Corallinales, Rhodophyta) from Brazil" addresses a topic that is relevant, innovative and suitable for this journal. The manuscript is, overall, well written and well reasoned.
It will have to be thoroughly reviewed in terms of the taxonomy of algae, as indicated below.
Note: Authors should always use valid names and not synonyms.
Corrections needed:
line 65 - antifouling activity of four GCA species: Amphiroa beauvoisii, Jania prolifera (formerly Amphiroa flabellata), Jania sagittata (formerly Cheilosporum sagittatum) and Jania crassa (Rhodophyta) from ...
line 71 - all extracts were active against marine bacteria, with J. sagittata (JsSI) extract being the most
line 72 - active. For the mussel Perna perna, the extract of C. sagittatum (JsSI) was the most active, with
line 76 - except in C. sagittatum extract (JsSI).
line 139 - species of GCA: Amphiroa beauvoisii J.V. Lamouroux, Jania prolifera A.B. Joly (formerly Amphiroa flabellata Harvey),
line 140 - Jania sagittata (J.V. Lamouroux) Blainville (formerly Cheilosporum sagittatum (J.V. Lamouroux) Areschoug) and Jania crassa J.V. Lamouroux
line 159 - beauvoisii, two of the species J. crassa, one of the species J. sagittata and one of the species
line 160 - J. prolifera. ...
line 171 - The extraction of each seaweed was performed three times, in which each interval had 2 h/16 h
line 172 - (overnight)/2 h maceration period, respectively. ...
line 173 - sonicated for 30 min. ...
line 182 - acetate (HPLC grade, Tedia) at a concentration of 1 mg/mL.
line 188 - at 300 °C for 5 min, totaling 33 min of analysis.
line 226 - were used as null control. Each plate was filled with 12 mL of seawater and three specimens.
line 227 - A total of 10 replicates were used for each treatment and control. After 24 h of
line 230 - a temperature of 22 ºC, salinity of 35 and constant aeration for 24 h.
line 256 - More complex profiles were observed in the extracts of J. crassa (JcP) and J. prolifera (JpPG),
line 257 - while the least complex were in the extracts A. beauvoisii (AbSCB) and J. sagittata (JsSI),
line 267 - (JcPA12.63%) and J. prolifera (JpPC19.49%). In the extract of J. sagittata (JsSI),
line 283 - J. crassa (JcP and JcPA), J. prolifera (JpPC) and J. sagittata (JsSI). On the other hand, the
line 288 - (AbPF), J. crassa (JcPA) and J. sagittata (JsSI). On the contrary, the positive axis grouped
line 290 - prolifera (JpPC). In addition to the two compounds already mentioned for PC1, the sterol ...
line 307 - The CJ. sagittata extract (JsSI) stood out from the others, presenting a significantly
line 316 - against mussels. In plates with J. sagittata extract JsSI) there was no byssus fixation, and its
line 334 - crassa (JcPA e JcP) and J. proliferaa (JpPC) species, (qualitative variation). On the other hand,
line 372 - Palmitic acid, also found in all extracts, being more abundant in the J. sagittata extract
line 376 - Jania spectabilis (Harvey ex Grunow) J.H. Kim, Guiry & H.-G. Choi (formerly Cheilosporum spectabile Harvey ex Grunow) ...
line 418 - microorganisms. Ethanolic extract of Jania pedunculata var. adhaerens (J.V. Lamouroux) A.S. Harvey, Woelkerling & Revier (formerly Jania adhaerens J.V. Lamouroux) showed a mild acti
line 426 - The extract of Jania sagittata (JsSI) presented one of the most satisfactory
line 429/430 - Methanolic extract of Jania spectabilis (formerly Cheilosporum spectabile) also showed activity against ...
line 430/431 - ... two species of diatoms Dickieia subinflata (formerly Navicula subinflata) and Nitzschia palea ...
line 433 - observed that the extract of J. sagittata inhibited the growth of four species of fungi and
line 455 - The results obtained for the extracts of the species A. beauvoisii, J. prolifera, J. sagittata and ...
The study investigated the chemical diversity and antifouling activity of geniculate calcareous algae. It's written in a good manner, however, before publishing, the author should check the typos, grammar, space, formatting etc, e.g. lines 94-95, line 108, lines 136-137, lines 139-140, line 173, etc.
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