Evaluation and characterization of indigenous rice (Oryza sativa L.) landraces resistant to brown planthopper Nilaparvata lugens (Stål.) biotype 4

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Ecology

Introduction

Rice (Oryza sativa L.) is consumed by more than one-third of the total human population across the globe as a major source of carbohydrates among cereal crops (Fitzgerald, McCouch & Hall, 2009; Jena et al., 2015). The brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), is one of the economically important insect pests identified in this crop, having an international significance (Normile, 2008; Heong & Hardy, 2009). This phloem sap feeder transmits rice ragged stunt virus (RRSV) and rice grassy stunt virus (RGSV), and holds the ability to cause more than 60% economic yield loss under favourable environmental conditions throughout Asia (Kumar, Maurya & Tiwari, 2012; Wei et al., 2019). Several chemical insecticides are registered to control rice BPH, but the unscientific and injudicious application of those products breaks the natural pest defender ratio in the field (Sarao & Mangat, 2014; Roy & Chakraborty, 2022). Therefore, the development of new varieties resistant to BPH is extremely important. The identification of new sources of resistance from landraces, wild cultivars or germplasms enables the plant breeders to amplify the resistance breeding program through genetic modification (Guo, Liao & Chuang, 2019). Modern high yielding varieties are primarily designed to meet the economic expectations of the farming communities and the demand of the rising populations, but these types do not have biotic stress resistance, especially for BPH. In several provinces of India where N. lugens outbreak occurs often (Andhra Pradesh, Odisha, Delhi, West Bengal and Punjab), this lack of resistance caused complete crop loss (Anant et al., 2021). Thus, in order to generate promising cultivars that provide persistent and targeted resistance to field populations of BPH, it is therefore required to find novel N. lugens resistance mechanisms. In comparison to developed varieties, folk rice or indigenous landraces have a diverse resistance features. The eastern and north-eastern provinces of India, known to have highly diverse rice landraces belonging to this region are expected to have high genetic variability for various pests and diseases including the BPH (Sinha, Mallick & Mishra, 2015; Anant et al., 2021; Babu et al., 2022). West Bengal, an eastern province of India, possesses many tribal belts. These tribal zones harbour many indigenous rice landraces, and well known to be one of the primary centres of origin of rice (Karmakar et al., 2012; Adak et al., 2020). However, evaluation and systematic exploration of most of the West Bengal rice landraces for their true genetic potential, and their characterization for BPH resistance were not yet done broadly.

The biophysical factors of a plant interfere with the feeding, orientation, mating or oviposition mechanisms of insects. In contrast, the biochemical factors are either the primary nutrients or secondary non-nutritional chemicals of plants that affect insect biology. Some of these non-nutritional chemicals are associated with feeding deterrence, repellence or toxicity on insects (Saxena, 1986). However, the potential nutritive factors of a plant also play a pivotal role in enhancing its resistance to insects, even in the absence of these chemicals (Mitchell et al., 2016). Host plant shows varied kind of reactions upon feeding and infesting by insects, and alteration of nutritional biochemistry of a plant also takes place in this response (Vanitha, Suresh & Gunathilagaraj, 2011). Thus, a strong basis for developing resistant varieties against BPH biotype 4 should be aligned towards ascertaining the resistance by imparting bio-chemicals and genetic resources and applying those as cues in the resistance breeding program.

In the present study, systematic phenotyping of traditional rice landraces of West Bengal was performed for BPH resistance over a continuous span of 3 years. Based on the phenotypic data, 40 promising landraces were further examined for antixenosis, antibiosis, and biochemical parameters concerning their host-plant resistance.

Materials and Methods

Plant and insect material

The present study was undertaken with a total of 218 rice landraces, native to different provinces of north-east India, previously registered under West Bengal Biodiversity Board, Department of Environment, Government of West Bengal, Salt Lake, Kolkata–700106, India (Table S1). The resistant Ptb33 and susceptible variety Swarna (MTU 7029) were considered as a positive and negative control, respectively, collected from National Rice Research Institute, Cuttack, Odisha-India germplasm unit. The insect culture of BPH biotype 4 was obtained from the Department of Zoology, University of Burdwan, West Bengal, India in 2017 and thereafter maintained in the insect-proof glasshouse of Bidhan Chandra Krishi Viswavidyalaya (22.9452° N, 88.5336° E), Nadia, West Bengal, India under the controlled conditions of 28 ± 2 °C, 75 ± 5% relative humidity and a photoperiod of 14:10 h (light: dark). Mass rearing was done on 30 days old rice plants in wooden cages of 120 × 75 × 150 cm dimensions having separate compartments tunicated with fine wire mesh on all sides. Fifteen to twenty adult gravid female BPH were collected with an aspirator and released on sowed plants of Swarna, previously placed in oviposition chamber. After 2 days of egg laying, the gravid females were recollected and shifted on another fresh set of Swarna plants for further oviposition. Plants containing eggs were taken out of cages with entire trays and shifted in another separate compartment for hatching of first instar nymphs. A new set of plants were provided inside the cubicle of nymphs according to the date of exposure of eggs. Similarly, adults were also maintained after emergence in the same or separate compartment as and when required. Both the nymphs of desirable instars and adults were used by culling from the specific compartments as per the various experimental quests. Likewise, a continuous pure mother culture of BPH was sustained throughout the period of investigation.

Phenotyping for BPH resistance

Primary screening of 218 rice landraces for BPH biotype 4 resistance was conducted under greenhouse and open-field conditions in 2017–2019. Cluster analysis of 218 rice landraces and two control varieties was done based on the phenotypic scores and two quantitative parameters, such as the number of BPH per three plants and percent chaffy grains. The similarity matrix was generated through the simple Euclidean distance across all parameters of different landraces, and this matrix was used in a hierarchical clustering technique of Ward’s minimum variance method using ‘R’ software, version 4.0.2 (R Core Team, 2020).

Greenhouse screening

The standard seedbox screening method of IRRI (2002) with suitable modifications by Jena et al. (2006) was followed for the free-choice test to evaluate BPH resistance to 218 rice landraces in a complete randomized design and replicated thrice. At the seedling three-leaf stage in the screening trays, 2nd instar nymphs of BPH in the rearing cages were released artificially onto the seedlings by visually ensuring the infestation of each seedling with at least 8–10 nymphs and were monitored at a regular interval for plant damage by BPH. When Swarna plants on one side exhibited intense damage, the entire cage was rotated by 180° for equal reaction on both sides. On the other hand, the ‘isolated cage-test method’ was followed by taking individual plastic pots (D × H, 8 × 20 cm) for each landrace to conduct no-choice screening (De Vos & Jander, 2008). Twenty freshly germinated seeds of each landrace were individually seeded concentrically in a single pot including Ptb33 and Swarna and encircled with a transparent OHP sheet-made hollow cylindrical structure (D × H, 6 × 30 cm), roofed with 80-mesh insect-proof net pieces at the top. Like the previous experiment, 2nd instar BPH nymphs were artificially released from the top by ensuring at least 35–40 individuals per pot. Both the experiments were terminated after the complete wilting of more than 90% of Swarna seedlings, and the damage to all landraces was computed individually. The score was taken based on 0–9 scale according to the international standard evaluation system (Horgan et al., 2015).

Open-field screening

Approximately 45–50 pre-soaked germinated seeds of each landrace, including both Ptb33 and Swarna, were individually sown into 1 m2 plot at a BPH biotype 4 hotspot (Burdwan district of West Bengal, India; 23.2324° N, 87.8615° E) (Krishnaiah & Varma, 2012). The plots were selected in a randomized complete block design throughout the field and replicated thrice (Bhogadhi & Bentur, 2015). Sowing was done deliberately late in the 2nd fortnight of July with a closer spacing (r-r × p-p, 15 × 15 cm) to get a maximum infestation of BPH (Satpathi, Chakraborty & Acharjee, 2012). Manual weeding operation was done at 25 and 45 days after transplanting (DAT) and a 15 cm water level was maintained followed by recommended agronomic practices except plant protection measures for standard BPH multiplication in the field. Phenotypic scoring was done by taking randomly selected 20 plants per replicated plot according to the damage scale 0–9 when Swarna plants exhibited ‘hopper burn’ symptoms (Sai Harini et al., 2013). Mean numbers of BPH nymphs and adults per three plants and chaffy grains (%) were also enumerated by following Timmanagouda & Maheswaran (2017).

Biophysical and biochemical study of rice landraces

The evaluation of different biophysical and biochemical parameters related to BPH resistance was conducted in a set of three replications with 40 promising rice landraces (see ‘Results’ section), selected from the three years phenotyping scores, in 2020–2021. Biophysical studies were conducted in a separate compartment of the insect-proof glasshouse of Bidhan Chandra Krishi Viswavidyalaya, whereas biochemical studies were carried out at the Indian Institute of Science Education and Research (IISER) Kolkata, West Bengal, India (22.9638° N, 88.5245° E). To determine the biochemical components, seeds were sown separately in two plastic containers for each tested landrace with no additional nutrient, and one set of 30 day-old seedlings was infested with 2nd–3rd instar BPH nymphs for 7 days. The green leaf sheaths of both healthy and BPH infested plants were used for the analysis of total phenol (TP), reducing sugar (RS), ascorbic acid (AS), oxalic acid (OA), crude silica (CS) and total free amino acid (TFA), while nitrogen (N), phosphorus (P) and potassium (K) were estimated from oven-dried (60 °C for 72 h) and ground plant materials.

Excretion of honeydew

Evaluation on the honeydew excretion of BPH was conducted with 30 day-old potted seedlings by following Sogawa & Pathak (1970), as described in our previous study (Roy et al., 2021). Five numbers of each 1-day-old adult females and 2nd instar nymphs were placed separately to the lower stem portion of the 30 day-old potted seedling with an orange coloured bromocresol green treated filter paper around the base and an inverted and basal perforated transparent plastic cup (80 ml volume) on the filter paper incarcerating the insects to the stem portion of about 9 cm long. The hole of the cup was closed with a ball of non-absorbent cotton and the honeydew droplets excreted by BPH were turned into blue spots when they came in contact with the filter paper after 48 h of insect imprisonment. The area marked with blue colour was measured on a millimetre squared (mm2) graph paper sheet as the extent of feeding and also interpreted statistically.

Settling behaviour

This experiment was carried out by taking 40 selected rice landraces seeded at random rows (10 seeds per row), 3.0 cm apart in a seedbox by following the standard procedure given by Sarao & Bentur (2016) as described in our previous study (Roy et al., 2021). The Swarna seeds were sown in two border rows, whereas a single row of Ptb33 was introduced in the middle of the box. The 2nd–3rd instar BPH nymphs with at least 12–15 individuals per seedling were introduced on 15 day-old rice landraces and the experiment tray was covered immediately with an insect-proof cage. Data on the number of nymphs settled on each seedling was recorded from randomly selected five plants in each row at 1, 3 and 5 days after release. In a parallel set of experiments, around 800 pairs of adults were released onto the 30 day-old seedlings, previously grown in a well puddled soil-filled tray, with the help of a giant aspirator under a free-choice fashion and similarly covered with an insect-proof cage. Numbers of adult males and females alighting on different landraces were visually counted at 6, 12, 24, 48, 72 and 96 h after release. The seedlings were manually disturbed after each observation in both cases for proper reorientation of the BPH nymphs and adults.

Nymphal survival

The experiment on nymphal survivability was carried out by caging 1-day-old freshly hatched 1st instar BPH nymphs on 15 day-old seedlings (20 nymphs per plant and replicated thrice) of all the landraces separately with fine muslin cloth ventilation (700,000 nanometer) along with Ptb33 and Swarna (Jena et al., 2015). Monitoring of the seedlings was done regularly for a consecutive 18 days, and the numbers of newly emerged adults were recorded and carefully removed from the seedlings. The percent of nymphal survival was enumerated using the formula of Heinrichs, Medrano & Rapusas (1985).

Percent(%)nymphalsurvival=numberofadultsemergednumberofnymphsreleased×100

Ovicidal test

Like the previous experiment, one mating pair of BPH adults (3-day-old) was confined to each tested rice seedling under a fine muslin ventilated cloth (700,000 nanometer), and replicated thrice. After 7 days the adults were removed and all the seedlings were observed for the hatching of nymphs from the day onward. The number of newly hatched nymphs was recorded and carefully removed from the plant using an aspirator. Seedlings were collected when nymphs stopped coming out after 15–18 days, and thereafter dissected under a stereoscopic zoom binocular microscope (40× magnifications) to examine the number of egg masses and the number of unhatched eggs. A total number of eggs were assumed to be the sum of the number of nymphs counted and the number of unhatched eggs. The percent unhatched eggs was enumerated by using the formula of Khan & Saxena (1985).

Percent(%)unhatchedeggs=numberofunhatchedeggs(numberofnymphsemerged+numberofunhatchedeggs)×100

Estimation of TP, RS and AS

The quantity of TP, RS and AS in healthy and BPH infested rice landraces were estimated by a calorimetric assay using a spectrophotometer (UV-1900; Shimadzu, Kyoto, Japan), dinitrosalicylic acid reagent (DNS) method and volumetric method, respectively, described by Sadasivam & Manickam (2008).

10mlcontain=x×10mgofglucose0.1=%ofreducingsugar

Absorbance corresponding to 0.1 ml of test sample = x mg of glucose.

Quantityofascorbicacid(mgper100gsample)=0.5mg×V2×100ml×100V1ml×5ml×Wt.ofthesamplewhere, V1 = known volume and V2 = titrated volume.

Estimation of OA, CS and TFA

Quantitative estimation of OA in healthy and BPH infested rice landraces was done by a direct calorimetric method with Indole reagent (Bergerman & Elliot, 1955), whereas the spectrophotometric method (UV-1900; Shimadzu, Kyoto, Japan) was used to estimate the CS (Wei-min et al., 2005) and TFA content (Moore & Stein, 1948).

Estimation of N, P and K

One gram each of oven-dried plant sample was taken from both healthy and BPH infested plants and N was estimated on a whole plant basis using the standard micro Kjeldahl method according to Piper (1966). For P and K, 250 mg of plant material was digested by wet digestion method (Piper, 1966) using a tri-acid mixture (nitric, sulphuric and perchloric acids in a 9:2:1 ratio) and the values were estimated with the help of a Systonics Digital Flame Photometer (Model S-931).

Statistical analysis

The data obtained from different experiments related to biophysical and biochemical parameters were analyzed using analysis of variance (ANOVA) with the help of IRRISTAT 4.0 software (Sarao & Bentur, 2016). Data were transformed using arcsine and square root transformations before being subjected to statistical analysis whenever required. Tukey’s HSD test (p ≤ 0.05) was done using SPSS software (version 18.0: Inc., Chicago, IL, USA) to compare the significance of statistics in the activities of each observed biophysical and biochemical parameters among the tested rice landraces. Pairwise correlation by Pearson’s correlation method and Principal Component Analysis (PCA) was employed to establish a relationship among different BPH resistance traits in the tested rice landraces using XL-Stats 2020 software at https://www.xlstat.com/en/ (accessed on 18.05.2022).

Results

Mass screening

The resistance scores were observed among 218 rice landraces ranged between 1.2 and 9.0 (greenhouse) and 1.1 and 9.0 (open-field), indicating a wide variation (Table S1). The five landraces viz. RL4, RL27, RL35, RL42 and RL56 were observed as resistant against BPH and grouped under the major cluster I, whereas most of the moderately resistant landraces were classified under cluster II (Fig. 1). Cluster III comprised 46 landraces closest to Swarna in the similarity matrix, however, the majority of moderately susceptible landraces constituted two sub-clusters under the major cluster IV.

Circular cluster dendrogram based on similarity matrix enumerated from 218 rice landraces (RL) and 2 control (Ptb33 and Swarna) varieties.

Figure 1: Circular cluster dendrogram based on similarity matrix enumerated from 218 rice landraces (RL) and 2 control (Ptb33 and Swarna) varieties.

Antixenosis and antibiosis study

Honeydew excretion

The quantity of honeydew excreted by BPH nymphs varied significantly among the tested landraces (Table 1). The lowest honeydew for BPH nymphs was recorded in RL35 (27.9 mm2), followed by RL4 (30.3 mm2), RL42 (30.7 mm2) and RL27 (33.3 mm2), respectively, and were at par to Ptb33. A similar trend of honeydew excretion was also observed for 1-day-old adult BPH females (Table 1).

Table 1:
Phenotypic reactions of selected rice landraces to BPH.
Type Overall remarks Area of honeydew (mm2) Settling behaviour Nymphal survival (%) Unhatched eggs (%)
Nymph Adult female Nymph Adult male Adult female
RL4 R 30.3 ± 0.12a 47.5 ± 0.37a 4.2 ± 0.03a 5.4 ± 0.09a 6.5 ± 0.05ab 25.6 (30.40)a 77.3 (62.58)c
RL27 R 33.3 ± 0.25a 59.4 ± 0.32a 6.4 ± 0.09a 5.7 ± 0.05a 6.8 ± 0.05ab 29.2 (32.71)a 84.1 (65.96)c
RL35 R 27.9 ± 0.28a 46.2 ± 0.24a 4.3 ± 0.06a 3.9 ± 0.06a 4.9 ± 0.05a 26.9 (30.40)a 70.8 (57.61)bc
RL42 R 30.7 ± 0.36a 49.1 ± 0.28a 3.2 ± 0.08a 3.0 ± 0.03a 5.9 ± 0.03ab 28.3 (32.96)a 81.2 (64.30)c
RL56 R 38.1 ± 0.71a 62.2 ± 0.48a 3.9 ± 0.03a 6.1 ± 0.05ab 4.8 ± 0.03a 32.7 (34.57)a 71.9 (57.99)bc
RL1 MR 37.3 ± 0.23a 69.5 ± 0.46a 9.2 ± 0.08ab 4.3 ± 0.05a 6.2 ± 0.04ab 41.3 (39.98)ab 72.2 (58.18)bc
RL190 MR 39.5 ± 0.53a 65.2 ±0.31a 6.2 ± 0.04a 6.5 ± 0.08ab 5.8 ± 0.07ab 46.2 (42.82)ab 68.7 (55.98)b
RL176 MR 34.9 ± 0.28a 81.2 ± 0.20a 5.1 ± 0.03a 4.3 ± 0.05a 6.8 ± 0.03ab 30.1 (33.27)a 53.5 (47.01)ab
RL23 MR 43.8 ± 0.18ab 72.5 ± 0.44a 4.6 ± 0.04a 4.6 ± 0.03a 5.6 ± 0.04ab 41.2 (39.93)ab 71.5 (57.73)bc
RL204 MR 40.1 ± 0.19a 65.8 ± 0.27a 5.6 ± 0.04a 3.6 ± 0.04a 4.9 ± 0.02a 32.8 (34.94)a 76.2 (60.80)c
RL54 MR 37.3 ± 0.23a 69.1 ± 0.33a 7.1 ± 0.10ab 4.1 ± 0.04a 5.8 ± 0.05ab 35.8 (36.75)ab 72.3 (58.24)bc
RL63 MR 40.1 ± 0.29a 64.2 ± 0.25a 7.3 ± 0.05ab 3.9 ± 0.04a 6.8 ± 0.07ab 27.9 (31.88)a 72.5 (58.37)bc
RL136 MR 38.2 ± 1.10a 67.2 ± 0.59a 4.3 ± 0.03a 4.1 ± 0.05a 6.2 ± 0.04ab 27.1 (31.37)a 58.6 (49.95)b
RL5 MR 63.5 ± 0.37ab 114.2 ± 0.79ab 7.3 ± 0.05ab 10.2 ± 0.14b 10.1 ± 0.09b 52.2 (46.26)ab 43.2 (41.09)ab
RL13 MR 70.7 ± 0.16b 138.3 ± 0.87ab 6.2 ± 0.06a 8.5 ± 0.06b 7.3 ± 0.05ab 36.9 (37.41)ab 49.2 (44.54)ab
RL192 MR 56.1 ± 0.71ab 176.2 ± 0.62b 8.6 ± 0.09ab 7.2 ± 0.05ab 7.3 ± 0.06ab 34.6 (36.03)ab 47.5 (43.57)ab
RL22 MR 61.1 ± 0.33ab 110.6 ± 1.12ab 8.9 ± 0.13ab 9.2 ± 0.16ab 10.2 ± 0.09b 42.2 (40.51)ab 49.4 (44.66)ab
RL166 MR 60.3 ± 0.25ab 120.8 ± 0.74ab 10.1 ± 0.08ab 8.1 ± 0.04b 9.5 ± 0.12b 54.9 (47.81)ab 50.2 (45.12)ab
RL58 MR 77.2 ± 0.41b 112.5 ± 0.41ab 7.3 ± 0.10ab 4.3 ± 0.04a 6.5 ± 0.03ab 34.2 (35.79)ab 58.4 (49.84)b
RL150 MR 49.5 ± 0.82ab 127.2 ± 0.39ab 7.6 ± 0.04ab 6.5 ± 0.06ab 8.6 ± 0.09b 43.2 (41.09)ab 54.9 (47.81)ab
RL44 MR 92.2 ± 0.53c 138.9 ± 0.72ab 10.1 ± 0.17ab 7.8 ± 0.11ab 11.2 ± 0.09b 31.2 (33.96)a 53.5 (47.01)ab
RL125 MR 56.2 ± 0.27ab 122.3 ± 0.66ab 6.2 ± 0.06a 7.1 ± 0.06ab 8.9 ± 0.14b 40.1 (39.29)ab 51.2 (45.69)ab
RL72 MR 50.1 ±0.32ab 112.3 ± 0.56ab 9.3 ± 0.05ab 4.2 ±0.03a 5.1 ± 0.03a 29.1 (32.64)a 58.2 (49.72)b
RL130 MR 49.0 ± 0.68ab 154.8 ± 1.17ab 7.0 ± 0.09ab 5.6 ± 0.07a 7.3 ± 0.07ab 42.8 (40.86)ab 49.0 (44.43)ab
RL81 MR 61.9 ± 0.18ab 161.3 ± 0.52b 9.2 ± 0.17ab 5.6 ± 0.06a 8.9 ± 0.04b 57.2 (49.14)ab 47.2 (43.39)ab
RL88 MR 59.1 ± 0.53ab 144.6 ± 0.82ab 9.3 ± 0.05ab 8.1 ± 0.10b 10.3 ± 0.08b 51.5 (45.86)ab 51.3 (45.74)ab
RL90 MR 62.9 ± 0.35ab 150.2 ± 1.41ab 7.3 ± 0.17ab 6.8 ± 0.08ab 8.2 ± 0.10b 29.1 (32.65)a 58.5 (49.89)b
RL110 MR 76.1 ± 0.51b 120.9 ± 0.74ab 8.1 ± 0.07ab 8.3 ± 0.09b 12.1 ± 0.09bc 45.8 (42.59)ab 55.6 (48.22)ab
RL186 MR 135.9 ± 0.69cd 190.2 ± 1.15b 12.3 ± 0.11b 10.1 ±0.04b 8.2 ± 0.07b 72.8 (58.56)b 38.2 (38.17)ab
RL199 MR 114.3 ± 1.06c 176.5 ± 0.94b 9.6 ± 0.10ab 7.6 ± 0.09ab 7.8 ± 0.08ab 58.1 (49.66)ab 39.6 (38.99)ab
RL20 MR 110.1 ± 0.82c 234.5 ± 0.89c 7.5 ± 0.05ab 6.5 ± 0.10ab 12.7 ± 0.15bc 52.1 (46.20)ab 42.2 (40.51)ab
RL118 MR 95.2 ± 0.40c 205.2 ± 1.26c 14.1 ± 0.11b 10.1 ± 0.09b 11.2 ± 0.07b 56.6 (48.79)ab 32.3 (34.63)a
RL33 MR 122.2 ± 0.46c 197.6 ± 0.62bc 9.6 ± 0.12ab 9.3 ± 0.16b 10.1 ± 0.06b 69.8 (56.66)b 38.2 (38.17)ab
RL162 MR 111.7 ± 1.14c 208.2 ± 1.52c 7.6 ± 0.03ab 11.2 ± 0.19b 10.2 ± 0.17b 53.6 (47.06)ab 31.2 (33.96)a
RL48 MR 109.7 ± 0.76c 238.7 ± 0.95c 10.5 ± 0.14b 8.1 ± 0.10b 12.2 ± 0.07bc 42.3 (40.57)ab 42.2 (40.51)ab
RL209 MR 119.2 ± 0.46c 224.0 ± 1.30c 12.5 ± 0.06b 9.5 ± 0.14b 11.6 ± 0.06b 75.8 (60.53)b 35.6 (36.63)a
RL156 MS 110.2 ± 0.56c 197.5 ± 1.28bc 13.2 ± 0.08b 10.1 ± 0.08b 8.2 ± 0.13b 81.1 (64.23)b 44.3 (41.73)ab
RL217 MS 129.6 ± 1.27cd 196.2 ± 0.67bc 12.1 ± 0.15b 10.1 ± 0.08b 9.1 ± 0.08b 76.6 (61.07)b 37.6 (37.82)ab
RL109 MS 114.5 ± 0.29c 210.9 ± 0.39c 10.1 ± 0.09ab 9.3 ± 0.17b 7.5 ± 0.07ab 51.2 (45.69)ab 35.4 (36.51)a
RL142 MS 127.9 ± 1.24c 218.5 ± 1.19c 9.3 ± 0.12ab 6.5 ± 0.05ab 9.1 ± 0.16b 72.8 (58.56)b 30.6 (33.58)a
Ptb33 R 38.2 ± 0.33a 71.1 ± 0.85a 3.8 ± 0.06a 2.9 ± 0.21a 4.5 ± 0.39a 26.6 (31.05)a 89.2 (70.81)cd
Swarna HS 212.6 ± 1.20d 389.2 ± 1.39d 16.0 ± 0.17b 15.4 ± 0.78bc 14.9 ± 1.36c 96.1 (78.61)c 24.8 (29.87)a
DOI: 10.7717/peerj.14360/table-1

Notes:

Data in parenthesis are shown as Mean ± SE.
The figures in parenthesis are transformed arcsine values.

R, Resistant; MR; Moderately resistant, MS: Moderately susceptible; HS, Highly susceptible.

The means indicated by different letters in a column are significantly different at p < 0.05 by Tukey’s HSD test.

Settling behaviour

The settling behaviour of BPH nymphs differed significantly among the tested landraces where the least number of nymphs settled on RL42, followed by Ptb33 and RL56 (Table 1). All most identical behaviour of nymphal settling was noticed on all the observation days. Overall, the number of nymphs settled 80.00% less on RL42, 78.12% on Ptb33 and 73.75% on RL4 concerning the susceptible check Swarna. The significantly lower number of adult males settled on RL42, Ptb33 and RL204, while Ptb33 and RL56 registered a significantly lower number of adult females of BPH (Table 1). The observations for both adult males and females were also found to be supplementary to the screening result of the landraces.

Nymphal survival

The mean percent survival rate of BPH nymphs on phenotypically resistant landraces was lower than on the Swarna (Table 1). The landraces such as RL4 (25.6%), RL35 (25.6%) and RL136 (27.1%) had the lowest survival rates, significantly different from Swarna (96.1%), but were statistically at par with Ptb33 (26.6%).

Hatching of eggs

Among the tested rice landraces, Ptb33 (89.2%), RL27 (83.4%), RL42 (81.2%) and RL4 (78.8%) exhibited significantly higher mean percent unhatched eggs of BPH, while Swarna registered the lowest percent (24.8%) of unhatched eggs and was not significantly different to RL118, RL162, RL109, RL209 and RL142 (Table 1).

Biochemical components

Total phenol, reducing sugar and ascorbic acid

The total phenol (TP), reducing sugar (RS) and ascorbic acid (AS) content in the leaf sheaths of the BPH infested and healthy rice plants were estimated and differed significantly among the tested rice landraces (Fig. 2). In the healthy plants, TP content was found to be 0.28 mg g−1 tissue in Ptb33, whereas Swarna exhibited 0.48 mg g−1 tissue. After the BPH infestation, a percent increase in TP content varied significantly among the resistant rice landraces in the range between 15.01% and 51.28%. A significantly higher quantity of RS was observed in moderately susceptible landraces, with the highest in Swarna (1.20 mg g−1 glucose equivalent), compared to Ptb33 (0.35 mg g−1 glucose equivalent). After BPH feeding, a percent decrease in RS was observed in the range of 1.32% to 65.71%, irrespective of all the rice landraces including control varieties. Healthy leaf sheaths of Ptb33 (1.15 mg g−1 tissue) followed by RL27 (1.06 mg g−1 tissue) registered the significantly highest amount of AS content compared to Swarna (0.65 mg g−1 tissue), but the percent reduction of AS was observed to be 23.48%, 14.15% and 13.85% in Ptb33, RL27 and Swarna, respectively, after the BPH infestation.

Relative variation in TP, RS and AS content before and 7 d after the BPH infestation in the selected rice landraces.
Figure 2: Relative variation in TP, RS and AS content before and 7 d after the BPH infestation in the selected rice landraces.

Oxalic acid, crude silica and total free amino acid

In the healthy rice landraces, a significantly higher range of oxalic acid (OA) content was observed in resistant and moderately resistant rice landraces (0.27–0.46 mg g−1 tissue) compared to Swarna (0.18 mg g−1 tissue) (Fig. 3). Percent reduction in OA was observed irrespective of all the rice landraces, including the controls, after BPH feeding. Besides, RL1 (17.52%) and RL63 (16.60%) exhibited significantly higher crude silica (CS) content, found to be statistically at par with RL23 (15.85%), and RL42 (15.80%) and Ptb33 (14.53%), compared to Swarna. However, a significantly higher percent decrease in CS content was observed in moderately susceptible landraces than in resistant ones (Fig. 3). The significantly highest quantity of total free amino acid (TFA) was observed in Swarna (2,148.2 µg g−1 of glutamic acid equivalent) followed by RL217 (2,041.7 µg g−1 of glutamic acid equivalent), compared to the resistant landraces before BPH feeding (Fig. 4). However, infestation resulted in a significant increase in the quantity of TFA among the tested landraces, except RL217 (−1.14%).

Relative variation in OA and CS content before and 7 d after the BPH infestation in the selected rice landraces.
Figure 3: Relative variation in OA and CS content before and 7 d after the BPH infestation in the selected rice landraces.
Relative variation in TFA content before and 7 d after the BPH infestation in the selected rice landraces.
Figure 4: Relative variation in TFA content before and 7 d after the BPH infestation in the selected rice landraces.

Nitrogen, phosphorus and potassium

The percent nitrogen (N) content was not varied significantly among the tested rice landraces, including controls, in healthy plants, while the BPH infested plants showed a significant variation (Fig. 5). Higher percent of N content was noticed in the moderately susceptible rice landraces (1.41% to 1.61%) with the highest in Swarna (1.72%), but the significantly lower range of N accumulation was observed in the resistant landraces (1.12% to 1.31%). However after BPH infestation, most of the resistant and moderately resistant landraces exhibited an increase in the percent phosphorus (P) content, except RL5 (−22.73%), and RL58 (−37.50%), RL209 (−31.71%) and RL136 (−5.77%). Additionally, a significant increase in potassium (K) content was observed in most of the rice landraces, however, RL209 exhibited a consistent rate in total K content before and after the BPH feeding (Fig. 5).

Relative variation in N, P and K content before and 7 d after the BPH infestation in the selected rice landraces.
Figure 5: Relative variation in N, P and K content before and 7 d after the BPH infestation in the selected rice landraces.

Correlation studies and principal component analysis

Pairwise correlation among the tested biochemical components quantified in various rice landraces indicated that the N was not significantly correlated with all the biochemical factors, except K (negatively) and TFA (positively) (Table 2). However, a significant and positive correlation was observed between TP and RS, K and OA, RS and TFA, AS and CS, and OA and CS. Additionally, N content in plants exhibited a significant and positive correlation with honeydew excretion and nymphal survival (Table 3). P was significantly but negatively correlated with nymphal survival, while percent un-hatched eggs, and settling of BPH nymphs and adult females showed a significantly positive and negative correlation, respectively, with both TP and K. Both OA and CS correlated significantly, but negatively, with honeydew excretion, settling of nymphs and adult females and nymphal survival. In contrast, honeydew excretion, settling of three BPH morphs and nymphal survival correlated significantly and positively with TFA (Table 3).

Table 2:
Pairwise correlation coefficient comparison of the tested biochemical components.
N TP P K RS AS OA CS TFA
N 1
TP 0.11ns 1
P −0.34ns 0.12ns 1
K −0.06* −0.01** 0.02ns 1
RS 0.74ns 0.09* −0.34ns -0.06** 1
AS −0.34ns 0.04ns 0.18ns −0.01ns −0.40ns 1
OA −0.61ns −0.10* 0.29ns 0.23* −0.78* 0.45ns 1
CS −0.61ns −0.32* 0.28ns −0.11* -0.73** 0.35** 0.69* 1
TFA 0.72* 0.42ns −0.34ns −0.04** 0.79* −0.41* −0.67** −0.76** 1
DOI: 10.7717/peerj.14360/table-2

Notes:

Significant at p < 0.05 level of significance.
Significant at p < 0.01 level of significance.

ns, Non-significant; N, Nitrogen; TP, Total Phenol; P, Phosphorus; K, Potassium; RS, Reducing Sugar; AS, Ascorbic Acid; OA, Oxalic Acid; CS, Crude Silica; TFA, Total Free Amino Acid.

Table 3:
Correlation between tested biochemical components and phenotypic reactions to BPH.
Parameters N TP P K RS AS OA CS TFA
Excretion of honeydew 0.59** −0.54* −0.46ns −0.72ns 0.66* −0.52ns −0.37** −0.67* 0.61*
Nymphal settling 0.54ns −0.76* 0.92ns −0.56** 0.75* -0.68ns −0.72** −0.59* 0.47*
Adult male settling 0.95ns 0.58* −0.57ns −0.37ns 0.69ns −0.51* −0.77* −0.87ns 0.66*
Adult female settling 0.62ns −0.64** 0.85ns −0.48* 0.61** −0.44ns −0.54* −0.68** 0.48**
Nymphal survival 0.81* −0.68* −0.67* −0.52* 0.78* −0.39ns −0.97** −0.81** 0.72*
Un-hatched eggs −0.48ns 0.71* 0.44ns 0.78** −0.42* 0.47** 0.80ns 0.73* −0.55ns
DOI: 10.7717/peerj.14360/table-3

Notes:

Significant at p < 0.05 level of significance.
Significant at p < 0.01 level of significance.

ns, Non-significant; N, Nitrogen; TP, Total Phenol; P, Phosphorus; K, Potassium; RS, Reducing Sugar; AS, Ascorbic Acid; OA, Oxalic Acid; CS, Crude Silica; TFA, Total Free Amino Acid.

For healthy plants, it was clear from the biplot that resistant landraces were closely associated with P, CS, AS, OA and K, while TP, TFA, N and RS had a close association with Swarna (Fig. 6). In BPH infested plants, CS and OA exhibited their close intimacy with the resistant landraces, however, TP shifted away from the negative control variety. Total variance explained and principal factor matrix for tested feeding attributing biochemical components of BPH on rice landraces have been tabulated in (Tables S2 and S3).

Scattered plot matrix score of healthy (A) and 7 d after BPH infested (B) rice landraces and tested biochemical components.
Figure 6: Scattered plot matrix score of healthy (A) and 7 d after BPH infested (B) rice landraces and tested biochemical components.

Discussion

The studies on rice resistance to BPH, one of the most notorious insect pests of rice in Asian countries, have been carrying out since long. Various traditional and wild rice varieties were identified as one of the major sources of resistant donors against BPH and reported by several workers through mass screening technique (Kalode & Khrishna, 1979; Jena et al., 2015). In the current study, the landraces with a score of ‘1’, neither preferred both nymphal and adult settling, encountered from the honeydew and settling tests, nor they were allowed for surviving and egg-laying (nymphal survivability and ovicidal test). These findings might be linked to the less ingestion of food and its improper usage impaired the development and survival of BPH on resistant varieties (Alagar et al., 2007). Feeding can only be determined preciously through computing the area of honeydew excretion and several studies recognized this method as the best for complementing the phenotypic screening (He et al., 2013). Various plant metabolites present within resistant rice cultivars inhibit the feeding activities of BPH due to the less preference and that was reflected in low honeydew excretion (He et al., 2013). Besides resistant cultivars, significantly lower amounts of honeydew excreted by BPH, when feeding on moderately resistant landraces, confirmed the accuracy of phenotypic screening (Singh, Sarao & Sharma, 2017) and the possible trend of resistance among the respective rice landraces (Ritu & Ravi, 2006). Soundarajan, Gunathilagaraj & Chitra (2002) also conceded that the enumeration of the feeding rate of BPH is a potential indicator to differentiate the resistant and susceptible genotypes of rice.

Results of the present study showed that a comparatively lower percent nymphal population of BPH survived on phenotypically resistant rice landraces than Swarna. These results were corroborated by the findings of Vanitha, Suresh & Gunathilagaraj (2011) and Kumar, Maurya & Tiwari (2012), who confirmed the reduced survival and longevity of BPH nymphs and adults on resistant and moderately resistant genotypes. Reduced and poor survival of BPH might be due to the lower feeding rate on resistant landraces, which may be attributable to the lack of phagostimulant or presence of antifeedant components (Seo et al., 2009; He et al., 2013; Sable, Suresh & Kumar, 2015). However, there could also be a great possibility of the inadequacy of essential plant nutrients in these rice landraces, required for BPH survival. In addition, several physiochemical factors of resistant landraces play an important role in insect herbivory by preventing ingestion of the adequate foods and nutrients required for insect survival (Bing et al., 2007; Syobu, Otuka & Matsumura, 2011). On the other hand, a significantly lower survival of BPH nymphs in a rice variety SD15 might be linked to the higher rate of unhatched eggs, and thus tends to be resistant to the diverse adaptabilities of the host (Zheng et al., 2017). However, antibiotic resistance levels in some resistant rice accessions were positively associated with the quantity of BPH feeding (Hao et al., 2008; Yang et al., 2017; Han et al., 2018). Therefore, different resistant factors among various selected rice landraces could be associated with the antibiotic reactions against BPH (Darshini & Sidde Gowda, 2015).

Nitrogen (N) content is regarded as an indicator of plant quality which was reported to induce a barrier against the resistance of BPH in rice (Salim, 2002; Lu & Heong, 2009). The higher quantity of honeydew excretion by BPH was obtained in susceptible cultivars, and N was significant and positively correlated with this behaviour. The synergistic relationship between N in rice leaf and higher feeding rate of BPH is possibly due to the ready-made succulence in leaf sheath for higher N content which may not affect the insect biology directly, but changes the host biochemistry and plays a significant role in the reduction of plant resistance (Rashid, Jahan & Islam, 2016). Results of the present study on the role and impact of N against BPH are also consistent with the elaborative findings of Lu et al. (2004, 2005) and Horgan et al. (2018). Besides N, phosphorus (P) and potassium (K) are also required by the herbivores for ATP and nucleic acid synthesis along with several physiological activities. A significant and negative impact of K on BPH feeding, settling, survival and reproduction observed in the present study, may be attributable to the distribution of primary metabolites in plant tissues, which in turn could affect the attractiveness of the plant for insects as well as their subsequent growth and development on it (Rashid et al., 2017a). However, some workers confirmed that a higher level of K was associated with a lower population of BPH due to the reduced level of reducing sugar (RS) and total free amino acid (TFA) in K rich rice cultivars (Vanitha, Suresh & Gunathilagaraj, 2011), and strongly boosted the correlation and principal component analysis (PCA) of the present investigation. The phenolic compounds were found to be the feeding deterrents to BPH in rice and generally have a positive correlation with host plant resistance (Singh, 2004). In the present study, a significantly lower level of total phenol (TP) was observed in the resistant landraces, compared to the susceptible check, where a higher percent increase in TP took place in the BPH infested resistant landraces. The implication of phloem chemistry of rice comprises silicic acid, oxalic acid and phenolic compounds, provokes resistance to BPH (Ghaffar, Pritchard & Ford-Lloyd, 2011), but the latter usually possess a negative impact over the formers (Ciulu, Cadiz-Gurrea & Segura-Carretero, 2018). Grayer et al. (1994) reported that higher silicon content in rice leaf sheath of a resistant variety can reduce the TP content at a lower level without disrupting the phenotypic resistance of the concerned rice variety to BPH. In addition, both oxalic acid (OA) and crude silica (CS) were already recognized as the sucking inhibitor against BPH in rice, and in the present experiment, a significant and positive correlation was observed between them. For BPH, reduced performance with impaired feeding behaviours and poor population growth on rice was recorded in higher silicon content cultivars by some previous workers (He et al., 2015; Reynolds et al., 2016; Yang et al., 2017) and strongly supported our findings. The possible mechanisms of plant resistance related to higher silicon content may be the increased rigidity and reduced digestibility of plant tissues due to a physical barrier formed from the higher deposition of silica in epidermal cells of resistant rice plants (Massey, Ennos & Hartley, 2006; Massey & Hartley, 2009; Han et al., 2015). Moreover, this physical barrier has the potential to reduce the food quality of herbivores and thus impair their feeding capability followed by the reduction of insect growth rate (Calandra et al., 2016). The TFA also played a significant role in BPH infestation on rice where most of the resistant landraces, including Ptb33, registered lower TFA content. It may be attributed that, resistant cultivars against sap suckers usually possess a lower quantity of TFA by limiting the nutritive value of plant tissues for the herbivores (Golan et al., 2017). Biplot of PCA suggests that TFA, RS and N content were in a close association in the healthy rice plants, while the distance between the former and two later was largest after BPH feeding. It was evident that the level of TFA content in rice leaf sheath increased after the BPH infestation which is consistent with the findings of Sempruch, Michalak & Leszczynski (2011). Although it is still not clear by the researchers regarding the mechanisms of resource allocation when attacked by herbivores, it can be hypothesized that higher cell damage would make the plant resource sequestration a possible preferred strategy (Orians, Thorn & Gomez, 2011). Moreover, Rashid et al. (2017b) linked higher K content with a lower level of TFA in the resistant rice plants and observed the increment of both the compounds after BPH feeding.

Conclusions

In conclusion, a high level of plant defence against BPH biotype 4, through antibiosis, antixenosis and biochemical bases of resistance, was exhibited by five phenotypically resistant rice landraces (RL4, RL27, RL35, RL42 and RL56), which may provide a useful tool for the plant breeders and biotechnologists to develop BPH resistant rice varieties. Besides, the restless behaviour of BPH on the phenotypically resistant rice landraces could enhance the chance of predation by the natural enemies in the field. Understanding the phenotypic expressions and biochemical mechanisms underlying resistance in rice landraces will contribute to the effective management of BPH biotype 4 and facilitate resistance breeding program more efficiently in future.

Supplemental Information

Supplemental Tables and Raw Data.

Raw data generated during the three years screening of 218 rice landraces in both greenhouse and open-field conditions, used to develop the cluster dendrogram.

DOI: 10.7717/peerj.14360/supp-1
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