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The paper can be accepted for publication in its current form.
[# PeerJ Staff Note - this decision was reviewed and approved by Vladimir Uversky, a PeerJ Section Editor covering this Section #]
English editing of the manuscript by a language expert is highly recommended. Two PDF files are attached for reviewers' comments.
[# PeerJ Staff Note: Please ensure that all review and editorial comments are addressed in a response letter and any edits or clarifications mentioned in the letter are also inserted into the revised manuscript where appropriate. #]
[# PeerJ Staff Note: The Academic Editor has identified that the English language must be improved. PeerJ can provide language editing services - please contact us at [email protected] for pricing (be sure to provide your manuscript number and title) #]
1. Unambiguous, professional English language used throughout.
2. Abstract: Minor updates needed
a. Methods: Add gist of study design details such as division of groups and treatment assignment etc.
b. Results: Consider adding brief statement about “clinical symptoms and mortality”
3. Introduction is adequate covering the area of research with recent references.
4. Material and Methods: Please see structure changes details
• Change sub-section 2.1 as Animals, also make the details of ethical committee at the end of paragraph as starting part.
• 2.2 should be Materials
• Consider adding new separate sub-section 2.3- Experimental design: explain the details
• 2.4 LORR measurement; adjust numbering of next sub-sections accordingly.
5. The results are well summarized.
6. The references used are Adequate - reference number 31 please add details of volume, issue and page numbers of the article.
7. Figures: Minor updates to description
• Fig 2: Consider adding statement of "No statistically significant difference in recovery time among the drugs.
• Fig 3: In the description, remove “for loss of the righting reflex.” Add statement of "Etomidate and ET-26HCl had a milder BP inhibition effect than propofol."
• Fig 4: In the description, remove “for loss of the righting reflex.” Add statement of “No significant differences were observed between control group and ET-26HCl group at 15, 30 and 60 min after ACTH stimulation.”
8. Table 1: changes required, please see bolded details
• Change heading from “Number of apneas” to “Number of aged rats with apnea.”
• Include unit of time (S) for Duration of apnea.
• In the description consider adding statement of "Apnea in all rats was observed in Propofol group; no apnea in Etomidate and ET-26HCl groups."
1. Interesting piece of work which would add to the evidence base in this field.
2. Research is substantial, and the results would contribute to the future development of clinical studies
3. Good information regarding methodology to replicate the study.
1. Manuscript sound scientific, along appropriate use of software to analyze the data.
2. Good sample size and appropriate statistical tests are employed for this analysis.
3. The coverage of the discussion is adequate. The conclusion is appropriate including limitations.
This article represents the importance and need of pre-clinical studies in aged rats to establish efficacy and safety of ET-26HCl. The authors supported their hypothesis/recommendation of clinical use ET-26 HCl in elderly population clearly.
To this end, my decision is approval of the manuscript with minor revisions.
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-Some ambiguity in terms of grammar
For example: Line 55, "similar cardiovascular stability and slight respiratory depression as etomidate, but mild adrenocortical inhibition" can be "was developed as a novel intravenous anesthetic with not lonly similar extent of cardiovascular stability and respiratory depression comparing to etomidate, but also milder adrenocortical inhibition."
eg2. Line 198 "Thus, the rats in this study can be translated to predict the potential advantages
199 of ET-26HCl in the elderly population" can be "Therefore, results of the current study can be translated to predict..."
-sufficient literature references
convincing data and reasonable experimental design in general
-mean and SD could be included in Figure 2
-please discuss briefly mechanisms and differences between etomidate and ET-26 hydrochloride
-limitations also include minimal evidence and calculations on EC50 and ED50
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