Sponges of Western Mediterranean seamounts: new genera, new species and new records

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Biodiversity and Conservation

Introduction

Seamounts are structures of high ecological and biological interest (Carvalho et al., 2020; Morato et al., 2013; Rogers, 2018), which provide excellent habitat for a rich communities of filter-feeding animals, such as corals, crinoids and sponges (Samadi, Schlacher & De Forges, 2007). These organisms are favored by enhanced currents, scarcity of fine sediment, accidented topography and predominance of hard substrata, features that characterize seamounts (White & Mohn, 2004). Sponges are ubiquitous on seamounts, where they tend to form dense and diverse aggregations that provide habitat and refuge to other animals like crustaceans, mollusks and fishes (Samadi, Schlacher & De Forges, 2007). Also, they are involved in benthic-pelagic coupling and recycling of nutrients, both processes of utmost importance in oligotrophic areas like the Mediterranean Sea, where they may contribute to the maintenance of higher trophic levels (De Goeij et al., 2013).

Despite their importance, very little is known about sponges of the Mediterranean seamounts, which is in contrast to the vast number of studies on sponge taxonomy available in other domains like the continental shelf or the submarine canyons (e.g. Vacelet, 1961, 1969; Pulitzer-Finali, Hadromerida & Poecilosclerida, 1978; Pulitzer-Finali, 1983; Boury-Esnault, Pansini & Uriz, 1994; Pansini, Manconi & Pronzato, 2011; Bertolino et al., 2015; Longo et al., 2018; Manconi et al., 2019; Enrichetti et al., 2020). However, in recent years the increase in the use of Remote Operated Vehicles (ROV) has facilitated the access and study of seamounts. Currently, information on sponges is available from the Erathostenes seamount in the Levantine Sea (Galil & Zibrowius, 1998), the Vercelli seamount in the northern Tyrrhenian Sea (Bo et al., 2011), the Ulisse and Penelope seamounts in the Ligurian Sea (Bo et al., 2020), the Avempace, Alboran Ridge, Seco de los Olivos and Cabliers seamounts in the Alboran Sea (Boury-Esnault, Pansini & Uriz, 1994; Pardo et al., 2011; Sitjà & Maldonado, 2014; De la Torriente et al., 2018; Corbera et al., 2019), and the Stone Sponge, Ses Olives, Ausias March and Emile Baudot seamounts in the Balearic Sea (OCEANA, 2011; Aguilar et al., 2011; Maldonado et al., 2015). However, most of these works adress the sponges at a community level, focusing on a general habitat characterization. Nonetheless, the studies adressing taxonomy have revealed that the Mediterranean seamounts are habitats for rare, poorly-known, or new species. For example, Aguilar et al. (2011) reported the carnivorous sponge Lycopodina hypogea (Vacelet & Boury-Esnault, 1996) at the Ausias March seamount, representing the first sighting of this species outside littoral caves. A singular reef formed by the Lithistid Leiodermatium pfeifferae (Carter, 1873) was recorded at the Stone Sponge seamount, being the first report of this species in the Mediterranean Sea (Maldonado et al., 2015).

Determining which species are present on a given seamount, and hence the seamount’s biodiversity is a first step towards the development of management plans to protect these habitats. It is also crucial to understand seamounts’ biocenosis, their structure and dynamics, how they can be affected by human disturbances, and to monitor potential biological invasions and long-term community changes (Clark et al., 2012; Danovaro et al., 2020).

Sponges are problematic as they are difficult to identify, which may lead to incorrect or underestimated biodiversity values. The use of molecular markers, a powerful tool to help in sponge identification, has shown that this group is much more specious than previously thought, and cryptic species are very common (Cárdenas, Perez & Boury-Esnault, 2012). Thus, detailed morphological descriptions supported by a complete genetic database are crucial for future studies.

The objective of this work was to improve the taxonomic knowledge on the sponges at three seamounts of the Mallorca Channel in the Balearic Islands: Ses Olives, Ausias March and Emile Baudot. Currently, these seamounts are being assessed for inclusion in the Natura 2000 network, under the scope of the LIFE IP INTEMARES project. One of the goals of this project is to improve the scientific knowledge of areas of ecological interest that harbor rich, vulnerable and protected habitats and species, which is necessary knowledge for the development of management plans. High abundance and diversity of invertebrates were observed during several surveys carried out in 2018, 2019, and 2020 at these seamounts, highlighting sponges as the dominant group. In the present paper we provide detailed descriptions of 18 demosponges and one hexactinellida, including a new genus and three new species, together with new descriptions and records of poorly-known taxa. For the new and dubious species, the sequences of two most used barcoding genes, the mitochondrial Cytochrome Oxidase subunit I (COI) and the nuclear 28S ribosomal fragment (C1-D2 domains), are also provided.

Materials and Methods

Study area

The Mallorca Channel is located in the Balearic Promontory (western Mediterranean Sea), between the islands of Mallorca and Ibiza. The area harbors three seamounts: Ses Olives (SO; 1°58′58.8″N, 38°57′36″E) and Ausias March (AM; 1°49′4.8″N, 38°44′49.2″E) located east of Ibiza and Formentera islands, and Emile Baudot (EB; 2°30′0″N, 38°43′55.2″E) located south of Mallorca and east of Ibiza-Formentera (Fig. 1). The seamounts SO, AM and EB are 375, 264 and 600 m high, respectively and 10 to 17 km long, with tabular summits elongated in NE-SW trends and located at 225–290, 86–115 and 94–150 m depth, respectively. SO and AM are of orogenic origin, emerging from depths around 900 and 600 m in their eastern sides and being separated from Ibiza and Formentera islands by depths around 600 and 400 m. By contrast, EB is a guyot of volcanic origin, which in its western side emerges from a plain around 900 m deep, with numerous fields of pockmark type depressions, located between SO and AM. At the eastern side of EB there is the so-called Emile Baudot scarpment, which descend down to 2,600 m deep and connects the EB to the abyssal plain of the Algerian sub-basin (between the Balearic Isands and the Algerian coast) (Acosta et al., 2004).

Map of the studied area showing the location of the sampling stations of beam trawl (BT), bottom trawl type GOC73 (GOC), rock dredge (RD) and remote operated vehicle (ROV). The characteristics of these sampling stations are shown in Table 1.

Figure 1: Map of the studied area showing the location of the sampling stations of beam trawl (BT), bottom trawl type GOC73 (GOC), rock dredge (RD) and remote operated vehicle (ROV). The characteristics of these sampling stations are shown in Table 1.

(A) General view of the Balearic Islands. (B) Detail of Ses Olives. (C) Detail of Ausias March. (D) Detail of Emile Baudot.
Table 1:
Details of the sampling stations.
Rsurvey Rstudy Year Sampling device Depth (m) Coordinates Area Seabed characteristics
Initial Final
206 1 2017 GOC-73 135 39°47′37.2″N
4°26′15.4″E
39°47′37.2″N
4°26′15.4″E
E Me Fishing ground, sedimentary bottom
20 2 2018 BT 275 38°56′6″N
1°57′58.3″E
38°56′6″N
1°57′43.9″E
SO Detrital bed of muddy sand
22 3 2018 BT 105 38°44′30.5″N
1°46′5.9″E
38°44′30.5″N
1°45′53.3″E
AM Rhodolith bed with invertebrates
51 4 2018 BT 128 38°44′53.9″N
2°30′41.4″E
38°44′58.9″N
2°30′54.7″E
EB Coarse sand with dead rhodoliths
60 5 2018 BT 138 38°43′13.1″N
2°29′29.4″E
38°43′5.5″N
2°29′20.4″E
EB Coastal detrital with sand
66 6 2018 BT 146 38°41′13.9″N
2°28′11.3″E
38°41′7.1″N
2°28′1.9″E
EB Coastal detrital with sand and small dead rhodoliths
52 7 2018 RD 109 38°44′13.2″N
2°30′3.6″E
38°44′12.5″N
2°30′12″E
EB Rhodolith bed
50 8 2019 BT 102 38°43′33.6″N
1°48′12.6″E
38°43′34.7″N
1°48′23.4″E
AM Rhodolith bed with invertebrates
99 9 2019 BT 131 38°46′20″N
1°48′54.7″E
38°46′29.3″N
1°49′36.1″E
AM Coastal detrital with sand and sponges
104 10 2019 BT 118 38°45′57.6″N
1°51′2.5″E
38°46′4.8″N
1°51′8″E
AM Coastal detrital
124 11 2019 BT 152 38°45′19.1″N
2°31′0.5″E
38°45′20.9″N
2°31′8.4″E
EB Detrital border
135 12 2019 BT 169 38°44′42.7″N
2°29′25.8″E
38°44′21.2″N
2°29′15.8″E
EB Detrital border with sand
136 13 2019 BT 147 38°44′42.7″N
2°29′25.8″E
38°43′13.1″N
2°29′21.5″E
EB Detrital border with gross black sand
166 14 2019 BT 433 38°44′3.1″N
2°28′12.7″E
38°43′44.4″N
2°28′1.2″E
EB Detrital mud
167 15 2019 BT 151 38°42′21.6″N
2°29′37.3″E
38°42′12.6″N
2°29′29.4″E
EB Detrital border with sand
175 16 2019 BT 410 38°46′21″N
2°30′44.3″E
38°46′31.1″N
2°31′5.9″E
EB Detrital mud
177 17 2019 BT 156 38°43′57.7″N
2°28′54.1″E
38°43′47″N
2°28′53.4″E
EB Detrital border with sand
95 18 2019 RD 275–220 38°47.8′0″N
1°52.6′0″E
38°47.7′0″N
1°52.4′0″E
AM Rocky slope
103 19 2019 RD 302–231 38°47.4′0″N
1°47.2′0″E
38°47.3″0″N
1°47.2′0″E
AM Rocky slope
224 20 2019 GOC-73 252 39°3′3.6″N
2°42′2.9″E
39°5′15.7″N
2°42′13.3″E
SW Ca Fishing ground, sedimentary bottom
225 21 2019 GOC-73 754 38°57′11.5″N
2°37′54.1″E
39°0′2.9″N
2°38′33″E
SW Ca Fishing ground, bathyal mud
1 22 2020 BT 289 38°58′0.5″N
2°0′22.7″E
38°58′14.9″N
2°0′0″E
SO Detrital with encrusting sponges and small crustaceans
17 23 2020 BT 113 38°45′15.5″N
1°46′53.4″E
38°45′4.7″N
1°46′36.1″E
AM Rhodolith bed with invertebrates
18 24 2020 BT 114 38°45′15.5″N
1°46′53.4″E
38°45′16.2″N
1°46′54.1″E
AM Rhodolith bed with invertebrates
45 25 2020 BT 147 38°42′51.8″N
2°30′13.7″E
38°42′28.1″N
2°29′24″E
EB Coarse sand and gravel with crustaceans and sponges
52 26 2020 BT 320 38°45′47.5″N
2°31′0.5″E
38°45′56.9″N
2°30′37.1″E
EB Organogenic sediments, shells rests and gravel with sponges
3 27 2020 RD 288–318 38°56′4.7″N
1°59′48.1″E
38°56′44.5″N
1°59′46.3″E
SO Rocks and rests of fossil Ostreids
7 28 2020 RD 325–255 38°58′41.9″N
1°59′2.4″E
38°58′33.6″N
1°59′8.5″E
SO Rocks, rests of fossil Ostreids and fossil corals
8 29 2020 RD 315–295 38°58′11.3″N
2°0′30.6″E
38°58′12″N
2°0′25.2″E
SO Rocks and rests of fossil Ostreids
14 30 2020 RD 325–270 38°55′33.6″N
1°58′5.6″E
38°55′45.1″N
1°58′1.2″E
SO Mud, rocks and fossil Ostreids
20 31 2020 RD 104–138 38°42′51.1″N
1°46′28.2″E
38°43′14.5″N
1°46′27.5″E
AM Rhodolith bed with sponges
27 32 2020 RD 222–195 38°47′31.2″N
1°52′43.7″E
38°47′28.7″N
1°52′31.8″E
AM Carbonated rocks with encrusting sponges and gravels
28 33 2020 RD 135–140 38°45′56.5″N
1°51′51.5″E
38°46′3.7″N
1°51′45.7″E
AM Rhodolith bed and rocks with sponges
43 34 2020 RD 118–116 38°44′25.1″N
2°30′40.3″E
38°44′26.9″N
2°30′33.5″E
EB Rhodolith bed and rocks with sponges
46 35 2020 RD 280–306 38°42′21.6″N
2°30′44.3″E
38°42′31.3″N
2°30′42.5″E
EB Basaltic rocks and fossil Ostreids with encrusting sponges
94 36 2020 GOC-73 142 39°1′13.8″N
2°51′2.5″E
39°2′16.8″N
2°49′43.7″E
SW Ca Fishing ground, sedimentary bottom
07_1 37 2020 ROV 249–122 38°45′44.7″N
1°46′0.8″E
38°45′22.3″N
1°46′22.1″E
AM Sedimentary slope and rhodolith bed with sponges
13 38 2020 ROV 465–352 38°48′22.3″N
1°52′57″E
38°48′26.3″N
1°52′39.4″E
AM Rocky slope with large sponges
23 39 2020 ROV 133–169 38°44′27.6″N
2°29′15″E
38°44′40.2″N
2°29′43.4″E
EB Rocky slope, rhodolith bed with sponges and corals
24 40 2020 ROV 150–134 38°44′46″N
2°29′28.3″E
38°44′57.5′N
2°29′54.2″E
EB Rocky slope and summit, rhodolith bed with sponges and corals
DOI: 10.7717/peerj.11879/table-1

Note:

(Rsurvey) reference number in the survey. (Rstudy) correspondent reference in the present study. (GOC-73) experimental bottom trawl net. (BT) beam trawl. (DR) rock dredge. (ROV) Remote Operated Vehicle Liropus 2000. (SO) Ses Olives. (AM) Ausias March. (EB) Emile Baudot. (E Me) eastern Menorca. (SW Ca) south-western Cabrera Archipelago.

The Algerian sub-basin hydrodynamics are mainly affected by density gradients, receiving warm and less saline Atlantic waters (Pinot, López-Jurado & Riera, 2002). These surface waters have high seasonal temperature variation, ranging from 13 °C during winter to 26 °C during summer, when a strong vertical temperature gradient is established between 50 and 100 m deep. The water column below this depth shows fewer variations than in other parts of the western Mediterranean Sea, being mainly influenced by the Levantine Intermediate Water (LIW). This water mass, originated in the eastern Mediterranean, has temperature and salinity around 13.3 °C and 38.5 ppt, respectively, and is situated approximately between 200 and 700 m deep, just above the Western Mediterranean Deep Water, which is located in the lowest part of the water column (Monserrat, López-Jurado & Marcos, 2008). The western Mediterranean Intermediate Water, characterized by lower temperature (~12.5 °C) because it is formed during winter in the Gulf of Lions by deep convection when sea-air heat flux losses are high enough, is found at 100–300 m deep, but does not reach the Mallorca Channel every year (Monserrat, López-Jurado & Marcos, 2008).

Within the general oligotrophy of the Mediterranean, the southern Balearic Islands waters in the Algerian sub-basin show more pronounced oligotrophy than waters of the Balearic sub-basin located north of the Archipelago, and above all than the adjacent waters off the Iberian Peninsula and the Gulf of Lions (Estrada, 1996; Bosc, Bricaud & Antoine, 2004). The lack of supply of nutrients from land runoff and the lower influence of shelf/slope fronts flowing along the Iberian Peninsula and the northern insular shelf edge could explain these differences (Massutí et al., 2014; and references cited therein).

Sampling

Sponge samples were collected at SO, AM and EB seamounts with a Jennings type beam trawl (BT) of 2 and 0.5 m horizontal and vertical openings, respectively, and a 5 mm mesh size cod-end, a rock dredge (RD) and the Remote Operated Vehicle (ROV) Liropus 2000 with an extensible arm. Sampling was performed during INTEMARES research surveys carried out in 2018, 2019 and 2020 on board of the R/Vs Angeles Alvariño and Sarmiento de Gamboa (Fig. 1). Additional samples from trawl fishing grounds of the continental shelf off Mallorca and Menorca were collected during the MEDITS research surveys carried out in 2017, 2019 and 2020 using the bottom trawl net GOC-73 of 2.5–3 and 18–22 m vertical and horizontal openings, respectively and a 10 mm mesh size cod-end, on board the R/V Miquel Oliver (Fig. 1). The sampling strategy of the MEDITS surveys is detailed in Bertrand et al. (2002) and Spedicato et al. (2019). BT and GOC-73 have been shown efective for sampling macro-benthic species of the epibenthic and nektobenthic communities of sedimentary bottoms, respectively (Reiss, Kröncke & Ehrich, 2006; Fiorentini et al., 1999; Ordines & Massutí, 2009). The SCANMAR and MARPORT systems were used to control the deployment and retrieval of both gears to the bottom. By contrast, RD and ROV were used for sampling rocky bottoms and steep slopes. A summary of sampling stations used in the present work can be found in Table 1.

On board, specimens were photographed and stored in absolute EtOH. External morphology, color and texture were annotated prior to conservation. Spicule preparations and histological sections were made according to the standard methods described by Hooper (2003). All the specimens were deposited in the Marine Fauna Collection (http://www.ma.ieo.es/cfm/) based at the Centro Oceanográfico de Málaga (Instituto Español de Oceanografía), with the numbers from CFM7356 to CFM7417 and CFM7450-CFM7451 (Table S1).

The electronic version of this article in Portable Document Format (PDF) will represent a published work according to the International Commission on Zoological Nomenclature (ICZN), and hence the new names contained in the electronic version are effectively published under that Code from the electronic edition alone. This published work and the nomenclatural acts it contains have been registered in ZooBank, the online registration system for the ICZN. The ZooBank LSIDs (Life Science Identifiers) can be resolved and the associated information viewed through any standard web browser by appending the LSID to the prefix http://zoobank.org/. The LSID for this publication is: (urn:lsid:zoobank.org:pub:47EC2384-A88C-4654-8425-A7A46BC47AC5). The online version of this work is archived and available from the following digital repositories: PeerJ, PubMed Central and CLOCKSS.

Morphological descriptions

Spicules were observed with a Nikon S-Ke optical microscope and photographed with a CMOS digital camera. Images were processed using the Fiji software (Schindelin et al., 2012). Whenever possible, at least 30 spicules per spicule type were measured. Spicules measures are written as length: min-average-max × thickness: min-average-max μm.Tangential and transversal thick sections were made with a scalpel and, if necessary, dehydrated with alcohol, mounted in DPX and observed under a compound microscope. Aliquots of suspended spicules were transferred onto foil, air dried, sputter coated with gold and observed under a HITACHI S-3400N scanning electron microscope (SEM).

Molecular analysis

DNA was extracted from a piece of choanosomal tissue (~2 cm3) using the DNeasy Blood and Tissue Extraction kit (QIAGEN). Polymerase chain reaction (PCR) was used to amplify the Cytochrome C Oxidase subunit I (COI; DNA barcoding) and the C1-D2 domains of the 28S ribosomal gen, with the universal primers LCO1490/HCO2198 (Folmer et al., 1994) and C1’ ASTR/D2 (Vân Le, Lecointre & Perasso, 1993; Chombard, Boury-Esnault & Tillier, 1998), respectively. PCR was performed in 50 μl volume reaction (34.4 μl ddH20, 5 μl Mangobuffer, 2 μl DNTPs, 3.5 MgCl2, 1 μl of each primer, 1 μl BSA, 0.1 μl TAQ and 2 μl DNA). The PCR thermal profile used for COI amplification was (94 °C/5 min; 37 cycles (94 °C/15 s, 46 °C/15 s, 72 °C/15 s); 72 °C/7 min). 28S amplification was carried out as detailed in Chombard, Boury-Esnault & Tillier (1998). PCR products were visualized with 1% agarose gel, purified using the QIAquickR PCR Purification Kit (QIAGEN) and sequenced at Macrogen Inc. (South Korea).

Sequences were imported into BioEdit 7.0.5.2. (Hall, 1999) and checked for quality and accuracy with nucleotide base assignment. Sequences were aligned using Mafft (Katoh et al., 2002). The resulting sequences were deposited in the GenBank database (http://www.ncbi.nlm.nih.gov/genbank/) under the following accession numbers: MW858346MW858351 and MZ570433 for COI sequences and MW881149MW881153 for 28S sequences; Table S1.

To assess the phylogeny of Foraminospongia balearica sp nov. and Foraminospongia minuta sp. nov., two different approaches were used: Bayesian Inference (BI) and Maximum likelihood (ML). Here, we selected closely related sequences belonging to the orders Agelasida, Axinellida, Scopalinida and Biemnida, obtained after a BLAST search (Altschul et al., 1990). Additionally, two sequences belonging to the order Suberitida were used as outgroup. A complete list of the used sequences is available at Table S1. BI and ML analyses were performed with the CIPRES science gateway platform (http://www.phylo.org; Miller, Pfeiffer & Schwartz, 2010) using Mr Bayes version 3.6.2 (Ronquist et al., 2012) and RAxML (Stamatakis, 2014). For Mr Bayes, we conducted four independent Markov chain Monte Carlo runs of four chains each, with 5 million generations, sampling every 1000th tree and discarding the first 25% as burn-in, while RAXML was performed under the GTRCAT model with 1000 bootstrap iterations. Convergence was assessed by effective sample size (ESS) calculation and was visualised using TRACER version 1.5. Genetic distance (p-distance) and number of base differences between pair of DNA sequences were estimated with MEGA version 10.0.5 software (Kumar et al., 2018).

Results

A total of 60 specimens belonging to two classes, nine orders, 13 families, 15 genera and 19 species were analyzed. All these species were collected at the Mallorca Channel seamounts, while three of them (Phakellia robusta Bowerbank, 1866, Petrosia (Petrosia) raphida Boury-Esnault, Pansini & Uriz, 1994 and Hemiasterella elongata (Topsent, 1928)) were also found at the continental shelf around Mallorca and Menorca. In situ images of some of these sponges, obtained with ROV from the seamounts of the Mallorca Channel, are shown in Fig. 2.

Remote Operated Vehicle (ROV) images of the sponge fauna from the seamounts of the Mallorca Channel, Ses Olives (SO), Ausias March (AM) and Emile Baudot (EB).

Figure 2: Remote Operated Vehicle (ROV) images of the sponge fauna from the seamounts of the Mallorca Channel, Ses Olives (SO), Ausias March (AM) and Emile Baudot (EB).

(A) Specimen of Polymastia polytylota collected at 409 m depth in AM. (B) Holotype of Foraminospongia balearica sp. nov. collected at 129 m depth in the AM summit. (C) Specimen of Phakellia ventilabrum collected at 132 m depth in the EB summit. (D) Uncollected specimen of Phakellia sp. at 374 m depth in the north knoll of AM. (E) Specimen of Haliclona (soestella) fimbriata collected at 131 m depth in the EB. (F) Rhodolith bed at 110 m depth in the summit of AM, with different sponge species, including F. balearica sp. nov. (arrow), (G) uncollected specimen of Calyx cf. tufa at 106 m depth in the summit of AM, (H) coralligenous bottom at 97 m depth in the summit of AM, with several sponges, including F. balearica sp. nov. (arrow).

Systematics

Phylum PORIFERA Grant, 1836

Class DEMOSPONGIAE Sollas, 1885

Suborder HETEROSCLEROMORPHA Cárdenas, Perez & Boury-Esnault, 2012

Order AGELASIDA Hartman, 1980

Family HYMERHABDIIDAE Morrow, Picton, Erpenbeck, Boury-Esnault, Maggs & Allcock, 2012

Genus Foraminospongia gen. nov.

(Figs. 2B, 2F, 2H, 3, 4, 5, 6; Table 2)

Foraminospongia balearica sp. nov.

Figure 3: Foraminospongia balearica sp. nov.

(A) Habitus of CFM-IEOMA-7356/i802 (holotype) in fresh state, with (B) Detail of the oscula and the dermal membrane (dm) and (C) Macroscopic view of the grooves at the skin.
Skeletal arrangement of Foraminospongia balearica sp. nov., CFM-IEOMA-7356/i802 (holotype).

Figure 4: Skeletal arrangement of Foraminospongia balearica sp. nov., CFM-IEOMA-7356/i802 (holotype).

(A–C) Tangential images of the surface, showing the dermal membrane (dm). (D–E) Transversal sections.
SEM images of the spicules from Foraminospongia balearica sp. nov. CFM-IEOMA-7358/i293_1 (paratype).

Figure 5: SEM images of the spicules from Foraminospongia balearica sp. nov. CFM-IEOMA-7358/i293_1 (paratype).

(A–D) Styles. (E) Rhabdostyles. (F) Oxea.
Foraminospongia minuta sp. nov.

Figure 6: Foraminospongia minuta sp. nov.

(A) Habitus of CFM-IEOMA-7362/i439 (holotype) on fresh state. (B) On deck image of CFM-IEOMA-7363/i474 (paratype). (C) Optic microscope image of the tangential skeleton of the holotype. (D) Schematic illustration of the choanosome of the holotype. (E–F) Styles. (G–H) Rhabdostyles.
Table 2:
Comparative characters of Foraminospongia balearica sp. nov. and Foraminospongia minuta sp. nov.
Specimen Style Rhabdostyle Oxea Depth Area
Foraminospongia balearica sp. nov.
CFM-IEOMA-7356/i802
Holotype
188–378–492 × 6–11–14 90–179 × 4–7 (n = 9) 456–609 × 9–11 (n = 3) 249–122 AM
St 13
CFM-IEOMA-7357/i144
Paratype
197–378–501 × 4–9–12 108–164 × 3–5 (n = 5) 249–493–656 × 4–8–12 (n = 15) 128 EB
St 4
CFM-IEOMA-7358/i293_1
Paratype
179–356–516 × 3–8–14 138–179 × 3–6 (n = 5) 328–527–763 × 3–8–13 127 AM
St 9
CFM-IEOMA-7361/i824_4
Paratype
177–403–634 × 5–9–13 92–165 × 3–6 (n = 9) 600 × 9 (n = 1) 133–169 EB
St 39
Foraminospongia minuta sp. nov.
CFM-IEOMA-7362/i439
Holotype
283–509–658 × 9–14–21 175–262 × 7–9 (n = 7) np 318–288 SO
St 26
CFM-IEOMA-7363/i474
Paratype
244–416–555 × 10–14–20 147–232 × 7–9 (n = 4) np 315–295 SO
St 28
DOI: 10.7717/peerj.11879/table-2

Note:

Depth (m), area (SO, Ses Olives; AM, Ausias March; EB, Emile Baudot) and sampling station (St; see Rstudy in Table 1) where these specimens were collected are also shown. Spicule measures are given as minimum-mean-maximum for total length × minimum-mean-maximum for total width. A minimum of 30 spicules per spicule kind are measured, otherwise it is stated. All measurements are expressed in μm. Specimen codes are the reference numbers of the CFM-IEOMA/and author collection. np, not present.

Type species

Foraminospongia balearica sp. nov.

Diagnosis

Hymerhabdiidae with massive, massive-tubular or bushy growth form, with styles, subtylostyles, tylostyles, and rhabdostyles. Besides, curved or angulated oxeas may be present. Ectosome with an aspicular dermal membrane supported by a plumoreticulated skeleton of styles, subtylostyles and tylostyles. Pores grouped into inhalant areas. Choanosome confusedly plumoreticulated.

Etymology

From the Latin foramen (pores) and spongia (sponge). The name refers to the fact that in both species, their skin has areas where pores are grouped, giving a characteristic macroscopical appearance.

Foraminospongia balearica sp. nov.

(Figs. 2B, 2F, 2H, F3, 4, 5; Table 2)

Diagnosis

Massive-tubular to bushy Foraminospongia, with styles, rhabdostyles and oxeas.

Etymology

The name refers to the Balearic Islands, the area where the species has been collected.

Material examined

Holotype: CFM-IEOMA-7356/i802, St 37, AM, ROV.

Paratypes: CFM-IEOMA-7357/i144, St 4, EB, BT; CFM-IEOMA-7358/i293_1, St 9, AM, BT; CFM-IEOMA-7359/i239 (not described), St 8, AM, BT; CFM-IEOMA-7360/i745 (not described), St 26, EB, BT; CFM-IEOMA-7361/i824_4, St 39, EB, ROV.

Specimens observed but not sampled: St 12, EB, BT; St 14, EB, BT.

Comparative material

Foraminospongia minuta sp. nov.: CFM-IEOMA-7362/i439, St 27, RD, SO; CFM-IEOMA-7363/i474, St 29, SO, RD.

Rhabderemia sp.: CFM-IEOMA-7415/i729_1 (only a slide deposited at the CFM-IEOMA), St 35, EB, RD.

Description

Massive-tubular or bushy sponges (Figs. 2B, 2F, 2H and 3A). Largest specimens up to 6 cm in diameter. When present, tubes are 2–3 cm in height and 1 cm in diameter. Sometimes several tubes are fused on another of its sides. Consistency slightly elastic, brittle, easily broken when manipulated. Surface smooth, rough to the touch. Color in life golden yellow, tan after preservation in EtOH. A translucid membrane is present, more evident near the oscula (Fig. 3B). Subdermal grooves forming a visible pattern (Fig. 3C). Circular oscula 0.3–0.6 cm. In most cases, oscula are placed at the end of tubes, however, the holotype also has a large osculum in the main body (Fig. 3B).

Skeleton

Ectosome characterized by a plumoreticulated tangential skeleton and a dermal membrane (Figs. 4A, 4B and 4C). In some areas of the dermal membrane there are small pores gathered. These porae areas correspond to the grooves that are perceptible to the eye. Choanosome, confusedly plumoreticulated with extensive spaces and ascending spicule tracts of 2–5 styles, sometimes protruding the surface. The tracts contain abundant spongin. In between the tracts transversal spicules are abundant (Figs. 4D4E).

Spicules

Styles (Figs. 3A3D): Fusiform, most gently curved, but sometimes abruptly curved once or twice. When the curvature is in the last portion of the spicule, they may resemble rhabdostyles. Roundish heads and sharp tips, sometimes telescoped, strongylote forms present. Swellings may happen at the head or below, sometimes barely visible, sometimes more patent, rarely tuberculated (Fig. 3D). Size range constant between specimensspecimens, not influenced by depth nor area (Table 2). They measure 177–375–634 × 3–9–14 µm.

Rhabdostyles (Fig. 3E): Uncommon. Abruptly curved below the head. Stylote, subtylote and tylote modifications present. Round head and acerated tips. They measure 90–143–179 × 3–5–7 µm. specimens

Oxeas (Fig. 3F): specimensCurved or bent, with one, two or several curvatures, sometimes slightly sinuous. Tips acerated or telescoped. They measure 249–520–763 × 3–8–13 µm. Their abundance varies between specimens.

Genetics

Two COI Folmer fragment sequences were obtained for the Holotype (CFM-IEOMA-7356/i802) and for one paratype (CFM-IEOMA-7358/i293_1) (Genbank id’s MW858346 and MW858347, respectively). Besides, we obtained a 28S sequence (C1-D2 domains) for the Holotype (GenBank id MW881153).

Ecological notes

The species is very abundant on the EB and AM, between 100 and 169 m (Table 2). It can be mainly found on rhodolith beds and sedimentary bottoms with gravel, together with other sponges like Poecillastra compressa (Bowerbank, 1866), Axinella spp., Halichondria spp. or some Haplosclerids, as well as with a very broad number of crustaceans and echinoderms. It was also collected down to 433 m (St 14).

Foraminospongia minuta sp. nov.

(Fig. 6; Table 2)

Diagnosis

Small, massive-encrusting and greyish in color Foraminospongia, with only styles and rhabdostyles as spicules.

Etymology

The name refers to the small size of the two collected specimens.

Material examined

Holotype: CFM-IEOMA-7362/i439, St 27, SO, RD.

Paratype: CFM-IEOMA-7363/i474, St 29, SO, RD.

Comparative material

Foraminospongia balearica sp. nov.: CFM-IEOMA-7357/i144, St 4, EB, BT; CFM-IEOMA-7358/i293_1, St 9, AM, BT; CFM-IEOMA-7356/i802, St 37, AM, ROV; CFM-IEOMA-7361/i824_4, St 39, EB, ROV.

Rhabderemia sp.: CFM-IEOMA-7415/ i729_1, St 35, EB, RD.

Description

Small massive-encrusting sponge (Figs. 6A and 6B), about 1.5 cm in diameter and 0.5 cm in height. Consistency: compressible and slightly crumby. Velvety surface. The holotype was brownish due to mud, the paratype was greyish, both in life and after preservation in EtOH. Translucent membrane that can be peeled off is present, with grooves forming a distinguishable pattern (Fig. 6B). A single, circular oscule is present on the holotype.

Skeleton

The ectosome consists of a tangential reticulation of styles (Fig. 6C), and some loose rhabdostyles.

The choanosome is a plumoreticulated net of styles, with some loose rhabdostyles (Fig. 6D).

Spicules

Styles (Figs. 6E and 6F): Fusiform, gently curved or straight. Heads roundish and swelled in most cases. Sharp tips. Most are tylota. Size range variable between the holotype and the paratype (Table 2). They measure 244–465–658 × 9–14–21 µm.

Rhabdostyles (Figs. 6G and 6H): Uncommon. Abruptly curved below the head, most with roundish, tylota modifications at the head and sharp tips. They measure 147–209–262 × 7–8–9 µm.

Genetics

Sequences of COI Folmer fragment and 28S C1-D2 domains were obtained for the holotype and deposited in Genbank under accession numbers MW858348 and MW881151, respectively.

Ecological notes

Both specimens were found at SO, between 288 and 318 m deep, associated to hard bottoms with fossil ostreids reefs.

Remarks on F. balearica sp. nov. and F. minuta sp. nov.

Regarding the interspecific variability of F. balearica sp. nov., the spicules of the studied specimens are in the same size range, except for the styles of the specimen from AM (CFM-IEOMA-7358/i293_1), which are shorter and thinner than those of the specimens from EB. Also, specimen CFM-IEOMA-7358/i293_1 has much more abundant oxeas than the others.

Regarding F. minuta sp. nov., the features of this species support the differential diagnostic characters of the genus Foraminospongia (plumoreticulated choanosomal skeleton, ectosome formed by a reticulation of spicules, dermal aspicular membrane with poral areas, presence of large styles and small rhabdostyles), but differs from F. balearica sp. nov. in its external morphology, being much smaller and massive-encrusting compared to massive-tubular or bushy and of a greyish color instead of golden yellow in the latter. Also, the spicular complement is different: F. minuta sp. nov. lacks oxeas and has longer and thicker styles and rhabdostyles. The differences in the size of the styles between the holotype and the paratype are notable, considering that both were collected at similar depths and habitats. These differences could suggest intraspecific variability for the spicule size within the species; however, more specimens are needed to corroborate this statement.

The morphological differences between the two species are backed by genetic results. The phylogenetic reconstructions for COI and 28S fragments show well-supported separation between the two F. balearica sp. nov. sequences and the F. minuta sp. nov. sequence. Between the two species, the differences in bp and p-distance (in percentage) for COI Folmer and the 28S fragments were 1 bp/0.2% and 1bp/0.1%, respectively.

Remarks on the genus Foraminospongia

The family Hymerhabdiidae was recently erected to include the genera Hymerhabdia, Prosuberites and some species of the polyphyletic genus Axinella and Stylissa (Morrow et al., 2019). Here, we propose Foraminospongia as a new hymerhabdiid genus. The main differences between Foraminospongia gen. nov. and both Hymerhabdia and Prosuberites are the growing habit, with Foraminospongia gen. nov. being massive, massive-tubular or bushy against encrusting. Also, it differs from Prosuberites in the presence of rhabdostyles and oxeas. However, the presence of rhabdostyles and oxeas is shared with Hymerhabdia, but the genetic differentiation between Foraminospongia and H. typica (type species of Hymerhabdia) is clear (Fig. 7). In addition, the ectosome with a dermal membrane and grouped pore areas of Foraminospongia is not present in any Hymerhabdia apart Hymerhabdia oxeata (Dendy, 1924) that has a dermal membrane, although neither Dendy nor the re-examination done by Van Soest & Hooper (1993) described pore areas. Therefore, H. oxeata could represent an intermediate stage between genuine Hymerhabdia and Foraminospongia species. However, the last statement is only speculative and must be checked in future works.

Phylogenetic tree topology for specimens of Foraminospongia balearica sp. nov., Foraminospongia minuta sp. nov. described in the present study and other related Agelasids.

Figure 7: Phylogenetic tree topology for specimens of Foraminospongia balearica sp. nov., Foraminospongia minuta sp. nov. described in the present study and other related Agelasids.

The three was constructed with Maximum likelihood and Bayesian inference, based on COI (A) and 28S (B) fragments. Posterior probabilities and bootstrap support values are shown at the nodes. A sequence of Suberites domuncula and Suberites ficus are used as outgroups in both trees.

As stated before, there are species of Axinella and Stylissa that are grouped inside Hymerhabdiidae. Although currently all these species are kept in Axinellida and Suberitida, respectively (Van Soest et al., 2021), they are phylogenetically related to Foraminospongia (see Fig. 7). To resolve this relatedness, we have included in the phylogenetic analysis sequences of A. damicornis (Esper, 1794), A. verrucosa (Esper, 1794), A. corrugata (George & Wilson, 1919), S. carteri (Dendy, 1889) and S. massa (Carter, 1887) used by Morrow et al. (2012) to define Hymerhabdiidae. The resulting trees show that those species are clearly different from Foraminospongia, which is corroborated by their morphology (Pansini, 1984; Hooper & Van Soest, 2002).

The genus Rhabderemia (Order Biemnida, family Rhabderemiidae) resembles Foraminospongia in having rhabdostyles and possessing a plumoreticulated choanosomal skeleton. However, most Rhabderemia also have peculiar rugose microscleres (thraustoxeas, spirosigmata, thraustosigmata, microstyles). To clarify the potential relatedness of Rhabderemia and Foraminospongia, we have included in the phylogenetic analyses the species Rhabderemia sorokinae Hooper, 1990, R. indica Dendy, 1905 and R. destituta Van Soest & Hooper, 1993. Moreover, we included in the COI tree one sequence of an encrusting Rhabderemia sp. (CFM-IEOMA-7415/ i729_1; Genbank ID MZ570433) collected at the EB, with spined rhabdostyles, toxas and spirosigmata (Fig. 7B). Other sequences of Biemnida available at the genbank have also been included (see Table S1).

The sequence of Rhabderemia sp. (CFM-IEOMA-7415/ i729_1; Genbank ID MZ570433) clustered together with R. sorokinae, a Great Barrier Reef sponge which also has spined rhabdostyles, toxas and spirosigmata, in addition to microspined microstyles, a fact that confirms that archetypical rhabderemids are not related to Foraminospongia. However, microscleres are lacking in R. mona (de Laubenfels, 1934) and R. destituta, so they resemble Foraminospongia. Rhabderemia mona is a Caribbean sponge described from bathyal depths off Puerto Rico, used to erect the genus Stylospira for “sponges having no spicules other than peculiar spirally twisted styles” (de Laubenfels, 1934). This single specimen was later studied by Van Soest & Hooper (1993) on a revision of the genus, who concluded that Stylospira should be considered a subgenus of Rhabderemia. Van Soest & Hooper (1993) also described R. destituta from the Galapagos Islands, a second species matching de Laubenfels’ diagnosis. Interestingly, apart from the lack of any kind of microscleres (even though de Laubenfels reported raphides for R. mona, not found by Van Soest & Hooper, 1993), both species had smooth rhabdostyles, just as Foraminospongia, which is in contrast to most of the other Rhabderemia spp. Among the 30 known species of the genus, only R. stellata (Bergquist, 1961), R. spirophora (Burton, 1931), R. gallica (Van Soest & Hooper, 1993), R. profunda (Boury-Esnault, Pansini & Uriz, 1994), R. africana Van Soest & Hooper, 1993, R. prolifera Annandale, 1915 and R. meirimensis Cedro, Hajdu & Correia, 2013 have smooth rhabdostyles.

Unfortunately, there are no sequences available for R. mona nor R. destituta, so their potential relatedness with Foraminospongia cannot be addressed. However, it should be noted that both species have only rhabstosytles as megascleres, wich is in contrast to the heterogenous set of megascleres shown by Foraminospongia (styles, tylostyles, subtylostyles, rhabdostyles and oxeas). This seems a strong argument against congeneric relatedness with Foraminospongia. However, this issue should be properly addressed in the future when sequences of R. mona and R. destituta become available.

Order AXINELLIDA Lévi, 1953

Family AXINELLIDAE Carter, 1875

Genus Axinella Schmidt, 1862

Axinella spatula Sitjà & Maldonado, 2014

(Fig. 8; Table 3)

Axinella spatula Sitjà & Maldonado, 2014.

Figure 8: Axinella spatula Sitjà & Maldonado, 2014.

(A) Photograph of fresh material deposited under CFM-IEOMA-7364-7366/i338_1A–1C. (B) Habitus of CFM-IEOMA-7366/i338_1C preserved in EtOH. (C) SEM images of the skeletal structure of CFM-IEOMA-7366/i338_1C with detail of the inner ectosomal layer, with trichodragmata (tri). (D) Long styles with (D1) subterminal swelling. (E) Regular shaped style. (F) Style with rhabdose modification. (G) Oxea asymmetrically curved. (H) Oxea centrocurved. (I) Oxea centrotylota. (J–K) Small oxeas.
Table 3:
Comparative characters of the collected specimens of Axinella spatula Sitjà & Maldonado, 2014, and those reported for the type material (Sitjà & Maldonado, 2014).
Specimen Styles Oxeas Trichodragmata Color Depth Area
MNCN-Sp145-BV33A
Sitjà & Maldonado (2014)
Holotype
165–1050 × 3–15 180–520 × 2.5–15 25–30 × 5–8 Beige after EtOH 134–173 Alboran Island
MNCN-Sp188-BV41A
Sitjà & Maldonado (2014)
Paratype
119–1400 × 4–15 190–750 × 5–20 25–35 × 5–8 Beige after EtOH 102–112 Alboran Island
MNCN-Sp57-BV21B
Sitjà & Maldonado (2014)
Paratype
245–1225 × 8–18 120–432 × 9–12 25–30 × 6–10 Black after EtOH 93–101 Alboran Island
CFM-IEOMA-7364/i338_1A
This work
349–613–1161 × 7–13–16 (n = 20) 187–374–507 × 5–11–16 32–39–47 × 5–7–10 Orange in life orange beige after EtOH 152 EB
St 11
CFM-IEOMA-7365/i338_1B
This work
248–900–1304 × 11–17–26 (n = 17) 219–377–485 × 7–11–16 36–45–56 × 5–7–8
(n = 9)
Orange in life orange beige after EtOH 152 EB
St 11
CFM-IEOMA-7366/i338_1C
This work
332–638–1265 × 4–12–17 (n = 23) 247–332–493 × 7–10–16 32–39–52 × 5–7–11 Orange in life orange beige after EtOH 152 EB
St 11
DOI: 10.7717/peerj.11879/table-3

Note:

Depth (m), area (EB, Emile Baudot) and sampling station (St; see Rstudy in Table 1) where these specimens were collected are also shown. Spicule measures are given as minimum-mean-maximum for total length × minimum-mean-maximum for total width (or as they appear in the cited texts). A minimum of 30 spicules per spicule kind are measured, otherwise it is stated. All measurements are expressed in μm. Specimen codes are the reference numbers of the CFM-IEOMA/and author collection for the Balearic specimens and the reference numbers of Invertebrate Collection of the National Museum of Natural Sciences (MNCN) of Madrid for Sitjà & Maldonado (2014) specimens.

Material examined

CFM-IEOMA-7364/i338_1A, CFM-IEOMA-7365/i338_1B and CFM-IEOMA-7366/i338_1C, St 11, EB, BT.

Description

Small, erect, cylindrical, and slightly flattened sponges, up to 3 cm height and 2–3 mm width (Figs. 8A8C). Very hispid all along the body. Orange in life (Fig. 8A) and orange beige after preservation in EtOH (Fig. 8B).

Skeleton

As in Sitjà & Maldonado (2014).

Spicules

Megascleres

Styles (Figs. 8D8F): with a wide size range, rounded ends and sharp tips. Straight or slightly curved. The largest ones may be slightly sinuous, sometimes with subterminal swellings (Fig. 8D1). Rhabdostyle modifications are present in small and intermediateintermediate stages (Fig. 8F). They measure 248–722–1304 × 4–14–17 µm.

Oxeas: curved or bent, sometimes centrotylote (Figs. 8G8K), with the curvature point at the center or displaced towards one of the extremities. Tips acerated. They measure 187–357–507 × 5–11–16 µm.

Microscleres

Raphides in trichodragmata (Fig. 8C, detail), abundant and of the same morphology in all specimens. They measure 32–40–56 × 5–7–11 µm.

Ecology notes

Found only on the north-eastern part of EB, at 152 m deep, on gravel bottoms with dead rhodoliths and with a large abundance of sponges such as P. (Petrosia) ficiformis (Poiret, 1789), P. (Petrosia) raphida Boury-Esnault, Pansini & Uriz, 1994, P. (Strongylophora) vansoesti Boury-Esnault, Pansini & Uriz, 1994 and several Tetractinellida.

Remarks

The specimens match well with those originaly described from the Alboran Sea. Balearic specimens are smaller (maximum height of 3 cm against maximum height of 10 cm in alboran specimens). Also, the size range of their styles and oxeas are not as wide as in Alboran specimens and trichodragmata of our specimens were always longer (Table 3).

Sitjà & Maldonado (2014) described two phenotypes, according to the color acquired after preservation in EtOH (black or beige). Also, they found skeletal variations linked to each group, corresponding to a higher or lower presence of short styles, the morphology of the trichodragmata or the skeletal arrangement. The specimens collected here correspond only to the beige phenotype.

With the present record, the species distribution widens towards the north-western Mediterranean Sea, since previously it was known only for the type’s location, at the Alboran Island (Sitjà & Maldonado, 2014).

Phakellia robusta Bowerbank, 1866

Synonymised names.

Phacellia robusta (Bowerbank, 1866) (misspelling of genus name)

Material examined

CFM-IEOMA-7367/i347_2, St 12, EB, BT; CFM-IEOMA-7368/i405 and CFM-IEOMA-7369/i409, St 15, EB, BT; CFM-IEOMA-7370/i414_2, St 16, EB, BT; CFM-IEOMA-7371/i417, St 17, EB, BT; CFM-IEOMA-7372/i712, St 25, EB, BT; CFM-IEOMA-7373/i731, St 35, EB, RD; CFM-IEOMA-7374/POR760, St 20, south-western Cabrera Archipelago, GOC-73; CFM-IEOMA-7375/POR762, St 21, south-western Cabrera Archipelago, GOC-73.

Ecology notes

The species was frequent at the studied area, being found in a broad depth range (150–750 m) on both rocky and sedimentary bottoms. In the trawl fishing grounds of the continental shelf around Mallorca and Menorca it was mostly found below 300 m deep, where most of the collected specimens were larger. In the seamounts of the Mallorca Channel, the species was common on gravel bottoms 150–170 m deep, where specimens tended to be very small (1.5–3 cm in height) and in rocky outcrops and vertical walls, where sizes were intermediate (4–12 cm in height) and large (20–35 cm in height).

Remarks

The species is reported for the first time in the Mallorca Channel, being its second record at the Balearic Islands, where it was previously recorded by Santín et al. (2018) from the Menorca Channel. In the Mediterranean, it is also known from the Gulf of Lions (Vacelet, 1969), the Tyrrhenian Sea (Topsent, 1925), the Alboran Sea (Maldonado, 1992), the Strait of Sicily (Calcinai et al., 2013) and the Adriatic Sea (D’Onghia et al., 2015). Besides, the species has been reported from several localities of the North Atlantic including the Gulf of Cadiz (Sitjà et al., 2019), the Azores Islands (Topsent, 1904), the Cantabrian Sea (Ferrer Hernández, 1914) and the North Sea (Bowerbank, 1866).

Phakellia ventilabrum (Linnaeus, 1767)

Synonymised names

Halichondria ventilabrum (Linnaeus, 1767)

Phacellia ventilabrum (misspelling of genus name)

Phakellia ventilabra (ruling of ICZN)

Spongia strigose Pallas, 1766 (genus transfer & junior synonym)

Spongia venosa Lamarck, 1814 (genus transfer & junior synonym)

Spongia ventilabra Linnaeus, 1767 (genus transfer & incorrect spelling)

Spongia ventilabrum Linnaeus, 1767 (genus transfer)

Material examined

CFM-IEOMA-7376/i822_1, St 39, EB, ROV.

Ecology notes

The single specimen was collected on a rhodolith bed in the summit of the EB at 132 m deep (Fig. 2C) where, according to preliminary analysis of ROV videos, it seems to be a rare species.

Remarks

This is the first report of the species at the Balearic Islands. The species has been widely reported in the North Atlantic (e.g. Alvarez & Hooper, 2002), to Greenland (Lundbeck, 1909; Hentschel, 1929) and Canada (Lambe, 1900). In the Mediterranean, it has been reported northern of the Iberian Peninsula (Uriz, 1984), in the Alboran Sea (Maldonado, 1992) and Corsica (Vacelet, 1961).

Phakellia hirondellei Topsent, 1890

Synonymised names

Axinella hirondellei Topsent, 1890 (reverted genus transfer)

Phakellia robusta var. Hirondellei (Topsent, 1890) (status change)

Tragosia hirondellei (Topsent, 1890) (reverted genus transfer)

Material examined

CFM-IEOMA-7377/i353, St 13, EB, BT; CFM-IEOMA-7378/i623, St 33, AM, RD.

Ecology notes

The species was found at two stations of similar depth (135–147 m) in AM and EB. Both stations are located at the border of the summit, an area that may be affected by enhanced water current and an increase in nutrient and food supply (Samadi, Schlacher & De Forges, 2007; Rogers, 2018). This could explain the common presence of large erect sponges such as Poecillastra compressa (Bowerbank, 1866) on stations located at these areas (personal observations).

Remarks

The species is reported for the first time in the Mallorca Channel, being its second record at the Balearic Islands, where it was previously recorded by Santín et al. (2018) from the Menorca Channel. In the Mediterranean Sea, it is also known in the north of the Balearic Sea (Uriz, 1984) and in the Gulf of Lions, the Ligurian Sea and Corsica (Fourt et al., 2017) and the Alboran Sea (Boury-Esnault, Pansini & Uriz, 1994).

Family HETEROXYIDAE Dendy, 1905

Genus Heteroxya Topsent, 1898

Heteroxya cf. beauforti

(Figs. 9, 10 and 11; Table 4)

Heteroxya cf. beauforti.

Figure 9: Heteroxya cf. beauforti.

(A) Habitus of CFM-IEOMA-7380/i726 in fresh state. (B) Habitus of CFM-IEOMA-7382/i461 in fresh state (large patch).
Schematic illustration of Heteroxya cf. beauforti skeleton in transversal section.

Figure 10: Schematic illustration of Heteroxya cf. beauforti skeleton in transversal section.

(A) General view. (B) Body arrangement on a thick area. (C) Body arrangement on a thin area. (ox I) oxea I. (ox II) oxea II. (bl) basal lamina. (ect) ectosome. (ch) choanosome. (tr) spicule tracks.
Spicules of Heteroxya cf. beauforti.

Figure 11: Spicules of Heteroxya cf. beauforti.

(A) Large oxeas I. (B) Small oxeas II. (C–D) Detail of polyactinal teratogenic modifications of oxeas II. (E) Drawing of a style with details of the tip (E1) and the head (E2).
Table 4:
Comparative characters of species of the genus Heteroxya.
Specimen Oxea I Oxea II Style Depth Area
Heteroxya corticata
Topsent, (1898)
Syntypes
redescribed by Morrow et al. (2019)
1600–1700–2000 × 26–32–37, Microspined ends 235–310–420 × 12–23
Pronounced spination (more at the tips)
np 1165–1240 Azores
Heteroxya beauforti
Morrow et al. (2019)
Holotype
622–1030–1385 × 10–16–21
Smooth
207–280–370 × 11–14–16
Smooth
5000–5650–6300 × 23–25–27 629–1469 Celtic Seas
Heteroxya cf. beauforti
CFM-IEOMA-7380/i726 434–569 × 7–13 (n = 7)
Smooth
107–180–287 × 4–6–9
Smooth
Broken 280–306 EB
St 35
CFM-IEOMA-7381/i444 319–467–580 × 6–10–14 (n = 23)
Smooth
104–171–257 × 4–6–8
Smooth (n = 23)
1151–3502/8–14 (n = 3) 288–318 SO
St 27
CFM-IEOMA-7382/i461 327–460–586 × 6–10–15
Smooth
167–233–286 × 3–7–9
Smooth
Broken 255–325 SO
St 28
CFM-IEOMA-7379/i727 420–530–623 × 9–12–15 (n = 18)
Smooth
142–192–293 × 6–8–10
Smooth
Broken 280–306 EB
St 35
CFM-IEOMA-7450 /i487 nm nm nm 270–325 SO
St 30
DOI: 10.7717/peerj.11879/table-4

Note:

Depth (m), area (SO, Ses Olives; EB, Emile Baudot) and sampling station (St; see Rstudy in Table 1) where these specimens were collected are also shown. Spicule measures are given as minimum-mean-maximum for total length × minimum-mean-maximum for total width. A minimum of 30 spicules per spicule kind are measured, otherwise it is stated. All measurements are expressed in μm. Specimen codes are the reference numbers of the CFM-IEOMA/author collection. np, not present; nm, not measured.

Material examined

CFM-IEOMA-7381/i444, St 27, SO, RD; CFM-IEOMA-7382/i461, St 28, SO, RD; CFM-IEOMA-7450/i487, St 30, SO, RD; CFM-IEOMA-7380/i726, St 35, EB, RD; CFM-IEOMA-7379/i727, St 35, EB, RD.

Description

Small encrusting patches, circular or irregular, up to 2 cm in diameter (Figs. 9A and 9B). Body less than 1 mm thick. Consistency hard and slightly flexible. Hispidation visible to the naked eye. Greyish in life and after preservation in EtOH. No pores observed.

Skeleton

A basal spongin layer adheres to the substrate and allows the whole body to be peeled-off with a scalpel. Just upon this layer there are Oxea II running parallel to the substrate. The choanosome has low spicule content. Choanosomal chambers are relatively well developed in the thicker parts of the sponge (Figs. 10A and 10B). Thick areas also have ascending tracts of Oxea II, with Oxea II placed in between. The choanosomal tracts are not present in the thinner areas (Fig. 10C). The basal layer and the choanosome have abundant circular bodies 3-9 μm in diameter, dark or transparent (Fig. 10B). The ectosome is constructed by a dense palisade of Oxea II, perpendicular to the surface, with Oxea I placed in the same perpendicular position, emerging towards the exterior. Long styles are found here and there outcrossing the ectosome and causing the hispidation.

Spicules

Oxeas I (Fig. 11A): may be gently curved or bent in the middle, with sharp tips. They measure 319–482–623 × 7–10–15 μm.

Oxeas II (Figs. 11B): gently curved, curved or bent in the middle. Some stylote modifications present. Many with teratogenic parts like bifid tips, swellings or poliaxonal modifications (Figs. 11C and 11D). They measure 104–198–293 × 3–7–10 μm.

Hispidating styles (Figs. 11E11E2): very long and thin, curved, with round ends and sharp tips. Most broken, only three complete from specimen CFM-IEOMA-7381/i444, measuring 1151–3502 × 8–14 μm (n = 3).

Ecological notes

The species has been collected on smooth basaltic rocks between 270 and 325 m deep at SO and EB, where it seems to be rather common. Mostly associated with other minute encrusting sponges like Hamacantha spp. or Bubaris spp.

Genetics

Sequences of COI Folmer fragment and the 28S C1-D2 domains were obtained from the specimen CFM-IEOMA-7380/i726. Both sequences were deposited at the Genbank, under the accession numbers MW858350 and MW881150, respectively.

Remarks

The genus Heteroxya contains two species, H. corticata Topsent, 1898 and H. beauforti Morrow, 2019. Heteroxya corticata is the type of the genus, known only from deep waters (1,200–1,600 m) of the Azores Archipelago. The species has two categories of oxeas, both microspined, and lacks styles. Conversely, H. beauforti is known from slightly shallower waters of Ireland (630–1,470 m), has smooth oxeas and posses long hispidation styles (Table 4). The genus was reviewed by Morrow et al. (2019), that sequence the COI of both holotypes. They found no differences between the COI of H. corticata and H. beauforti but conclude that morphological differences were enougth to consider both as different species.

Morphologically, our material is more related to H. beauforti due to the abscense of microspined oxeas and the presence of hispidation styles. We have found circular bodies embedded in the choanosome and the basal layer, which can be equivalent to the spherulous cells found in H. beauforti (Morrow et al., 2019). However, oxea I, oxea II and styles are markedly shorter and thinner in our material than those of H. beauforti. Those differences may be a result of depth, nutrient, or temperature differences. On the other hand, the COI sequence of our material is identical to the sequences of H. corticata and H. beauforti. We have sequenced the 28S C1-D2 domains, but there are no published sequences to compare. Considering the lack of genetic differences and the affinity of our material to H. beauforti, here we believe that erecting a new species is not justified. Future works using other markers will clarify if H. cf. beauforti and H. beauforti are conspecific, or if H. cf. beauforti is a different species.

Heteroxya cf. beauforti represents the first record of a species belonging to the genus Heteroxya in the Mediterranean Sea.

Family STELLIGERIDAE Lendenfeld, 1898

Genus Paratimea Hallmann, 1917

Paratimea massutii sp. nov.

(Fig. 12; Table 5)

Paratimea massutii sp. nov.

Figure 12: Paratimea massutii sp. nov.

(A–B) Habitus of the holotype CFM-IEOMA-7383/i403 in fresh state, on its upper (A) and lower (B) sides. (C) Habitus of the paratype CFM-IEOMA-7384/i420 preserved in EtOH. (D) Schematic illustration of a transversal section of the holotype. (E–J) SEM images of the Holotype. (E) Oxea I, (F) Oxea I (auxiliar spicule). (G–J) Oxyasters (all with same bar scale).
Table 5:
Comparative characters of Paratimea spp. from the Mediterranean and the north-eastern Atlantic, including Paratimea massutii sp. nov.
Species/specimen Megascleres Accessory oxeas Oxyaster Other spicules External morphology Depth Area
Paratimea massutii sp. nov.
CFM-IEOMA-7383/i403
Holotype
Oxeas
910–1419–1711 × 16–24–33 (n = 17)
469–681–827 × 3–8–10 (n = 7) Smooth,
25–36–45
9–25 rays
np Massive, lobate surface, whitish with diatom brownish on the upper side 151 m EB
St 15
CFM-IEOMA-7384/i420
Paratype
Oxeas
1130–1374–1561 × 11–20–28
556–755–862 × 3–6–8 Smooth,
27–39–57
7–20 rays (occasionally 2 rays)
np same as i403 156 m EB
St 17
Paratimea oxeata Pulitzer-Finally, (1978)
Holotype 1000–1450 × 14–24 250–650 × 3–7 40–60 np Thickly encrusting, up to 4 × 5 × 0,4 cm, drab color in life, white after formalin and EtOH 60 and 100–110 m Bay of Naples
Bertolino et al. (2013) 810–961–1200 × 15–18–25 300–547–700 × 3–5–5 25–42–60 np Very small (0.5 cm2) insinuating sponge, grey colored in dry state. 35 m Ligurian Sea
Morrow et al. (2019) 1000–1500 × 14–24 250–650 × 3–7 20–40 but up to 60 when reduced rays
4–12 rays
np Massive lobose, surface
conulose, oscules arranged on top of raised humps, Pale yellow-cream
Caves, 15–20 m Gulf of Lion
Paratimea loricata (Sarà, 1958a)
Holotype Oxeas, poliaxonic and aberrant terminations. Mostly non-centrotylota.
320–420 × 5–7 (most common)
and 600 × 15 (n = 1)
Centrotylote
105–180 × 2–3
Large: 40–50
Small (uncommon): 12–20
Tylostyles, trilobated head
130–170 × 4–7
Encrusting, elastic but friable, whitish-yellow after EtOH Not specified, infralittoral Ligurian Sea
Paratimea pierantonii (Sarà, 1958b)
Holotype and paratypes Styles and Subtylostyles: 1530–2550/12–18 650–1175 × 4–10, centrocurved, non-centrotylote 15–25 np Cushion shaped with papillae. Hispid, smooth to the touch. Orange yellow at the surface, brownish inside. 30 cm, tidal cave Tyrrhenian Sea
Paratimea arbuscula (Topsent, 1928)
Holotype Curved or flexuous, centrotylote. Some modified to styles. 560–1000 × 5–12 nr Without centrum, with conical, acanthose actines,
15–60
most with 12 rays
np Small arbuscular sponge, up to 1 cm in heigth 1 mm in width, hispid. Whitish. Asters concentrated at the periphery 650–914 m Azores
Paratimea duplex (Topsent, 1927)
Reproduced from the redescription in Morrow et al. (2019) Centrotylote oxeas 2000–2600 × 20–40, styles to subtylostyles 1600–1800 × 25–35 Weakly centrotylote
360–770 × 7–9
Without
centrum, smooth rayed,
50–100
10–15 rays
np cushion shaped, 3 mm
thick, with a conulose surface
240–2165 m North Atlantic Ocean
Paratimea constellata (Topsent, 1904)
Holotype, reproduced from Morrow et al. (2019) Long, slender tylostyles
2500–3000 × 13–14
Centrotylote oxeas 379–670–900 × 8–10 Smooth-rayed euasters 14–30–46 np Cushion shaped, 2–3 mm thick, yellow gold 40 m Roscoff, Celtic seas
Paratimea loenbergi (Alander, 1942)
Reproduced from the redescription of the Holotype in Morrow et al. (2019) 1350–3000 × 10–13–15 (n = 4); head, 16–20–27 Slightly bent, 530–712–930 × 5–5–6 (n = 7) Smooth 22–28–36 Small category of tylostyles not found by Morrow et al. (2019) but mentioned in the original description, measuring 180–225 × 12–15 Thin, hispid
crust, pale yellow.
60 m Väderöfjord, Sweden
Paratimea hoffmannae Morrow & Cárdenas, 2019
Holotype, original description Large, curved oxeas, occasionally
centrotylote
2056–2187–2250 × 25–26–28
Rare, bent, occasionally centrotyle
353–446–520 × 3–4–5
Asymmetic 42–60–81 μm
7–18
smooth, tapering rays
np Massive, subspherical.
Holotype is ~7 in diameter. Surface covered in large conules,
1–4 mm in height. Creamish white.
328 m (Holotype)
1500 m (Paratype)
Norway (Holotype)
Ireland (Paratype)
DOI: 10.7717/peerj.11879/table-5

Note:

Depth, area (EB, Emile Baudot) and sampling station (St; see Rstudy in Table 1) where these specimens were collected are also shown. Spicule measures are given as minimum-mean-maximum for total length × minimum-mean-maximum for total width. A minimum of 30 spicules per spicule kind are measured, otherwise it is stated. All measurements are expressed in μm. Specimen codes are the reference numbers of the CFM-IEOMA/author collection. np, not present; nr, not reported.

Diagnosis

Massive ovoid sponge with oxeas as megascleres and oxeas as auxiliary spicules. Centrotylotism occasionally present in both. Oxyasters smooth.

Etimology

Dedicated to Professor Enric Massutí, for his contribution to the knowledge of the benthic communities of the Balearic Islands.

Material examined

Holotyope: CFM-IEOMA-7383/i403, St 15, EB, BT.

Paratype: CFM-IEOMA-7384/i420, St 17, EB, BT.

Description

Both specimens are massive, subspherical, the largest (holotype, CFM-IEOMA-7383/i403; Fig. 12A) measuring about 5 cm in diameter, having a lobose surface with grooves and humps. Skin of a leathery touch, hispid only in the grooves. Color in life differing between the upper and the lower faces, the former having the first a brownish tinge while the latter a whitish to beige shade (Figs. 12A and 12B). After preservation in EtOH the whole body turns homogeneous vanilla cream (Fig. 12C). Both specimens have 4–6 circular oscula, 1–2 mm in diameter, scattered throughout the body. However, the holotype also has a main large and circular osculum, about 1 cm in diameter, on the upper side. Both specimens expelled a considerable amount of mucus when collected.

Skeleton

Ectosome not separable from the choanosome, formed by a dense crust of oxyasters and tangential principal and auxiliary oxeas. Choanosome composed of irregularly arranged oxeas and oxyasters, although radial bundles of large oxeas are present in the periphery, supporting the ectosome (Fig. 12D).

Spicules

Megascleres

Oxea I (Fig. 12E): robust and fusiform, some double bent, sometimes slightly centrotylote. They measure 910–1390–1711 × 11–21–33 μm.

Auxiliary spicules

Oxea II (Fig. 12F): uncommon. Bent or slightly sinuous, sometimes centrotylote. They measure 469–746–1088 × 3–7–10 μm.

Microscleres

Oxyasters (Figs. 12G12J): with long, smooth and sharp rays. About 7–25 rays, occasionally less. Smaller ones tend to have more rays than larger ones, measuring 25–38–57 μm. Occasionally, some two-rayed oxyaster present.

Ecological notes

Found at two stations on calcareous gravel bottoms on the summit of EB (155 and 167 m deep), which was dominated by sponges such as Hexadella sp., Phakellia robusta and different species of the order Tetractinellida. A large number of the brachiopod Gryphus vitreus (Born, 1778) and echinoderms were also recorded.

Genetics

Sequences of COI Folmer fragment was obtained from the Holotype (CFM-IEOMA-7383/i403) and deposited at Genbank under the accession number MW858351.

Remarks (see Table 5 for a detailed comparison with other Paratimea spp.)

Morphologically, the species resembles Paratimea oxeata Pulitzer-Finali, Hadromerida & Poecilosclerida, 1978, a Mediterranean species reported at rocky and muddy bottoms, at 35–60 and 110 m deep, respectively (Pulitzer-Finali, Hadromerida & Poecilosclerida, 1978; Bertolino et al., 2013), and at submarine caves at 15–20 m deep (Morrow et al., 2019). However, P. massutii sp. nov. is massive, a feature only shared with the cave specimen (S153) reported by Morrow et al. (2019). Notwithstanding, in P. massutii sp. nov. oxeas I are thicker, oxeas II longer and oxyasters slightly larger and with more actines (2–25 vs. 4–12). A comparison of the COI sequences between the holotype of P. massutii sp. nov. and the cave specimen confirms those morphological differences, with 15 bp differences and a p-distance of 2%. On the other side, both the holotype and the specimens studied by Bertolino et al. (2013) differ from P. massutii sp. nov. in being cushion shaped or encrusting and having smaller oxeas. Unfortunately, no sequences of Bertolino et al. (2013) specimens are available to compare. Paratimea massutii sp. nov. is also similar to P. hoffmannae Morrow et al. (2019), a North Atlantic species found in Norway and Ireland that is also massive and subspherical and has oxeas as both megascleres and auxiliary spicules. However, the large oxeas are much larger and thicker than in P. massutii sp. nov., in contrast to the auxiliary spicules, which are shorther and thinner. Also, the oxyasters of P. hoffmannae are larger and with less actines. As for P. oxeata, COI sequences between P. hoffmannae and P. massutii sp. nov. are notably distant, with 13 bp differences and a p-distance of 2%. A similar case happens with P. lalori Morrow, 2019 from Ireland. This species is also massive-subspherical with oxeas as main megascleres and auxiliary spicules. Just as in P. hoffmannae, megascleres of P. lalori are longer and thicker than those of P. massutii sp. nov., auxiliary spicules are shorter and thinner and oxyasters slightly larger and with fewer actines.

Paratimea massutii sp. nov. also differs from the other Mediterranean Paratimea spp. as follows: P. loricata (Sarà, 1958a) is encrusting, has much smaller oxeas I and oxeas II and two categories of oyasters, and bears tylostyles; P. pierantonii (Sarà, 1958b) is cushion-shaped, has styles and subtylotyles as megascleres, longer, thicker, and never centrotylote oxeas II and smaller oxyasters.

Also, P. massutii sp. nov. differs from North-eastern Atlantic Paratimea spp. as follows: P. constellata is cushion shaped, has tylostyles and smaller oxyasters; P. arbuscula (Topsent, 1928), is arbustive, lacks auxiliary spicules and has smaller, acanthose oxyasters; Paratimea duplex (Topsent, 1927) is cushion shaped, has much larger oxeas I, bears styles, subtylostyles, and two categories of oxyasters; P. loennbergi (Alander, 1942) is thinly encrusting, has tylostyles and smaller oxyasters.

This is the first report of the genus Paratimea in the Balearic Islands, and the deepest record in the Mediterranean Sea.

Order BUBARIDA Morrow & Cárdenas, 2015

Family BUBARIDAE Topsent, 1894

Genus Rhabdobaris Pulitzer-Finali, 1983

Rhabdobaris implicata Pulitzer-Finali, 1983

Synonymised names

Cerbaris implicatus (Pulitzer-Finali, 1983)

Material examined

CFM-IEOMA-7385/i338_2_1, St 11, EB, BT; CFM-IEOMA-7386/i698, St 34, EB, RD.

Ecological notes

Uncommon sponge found at two stations on the EB summit at 117 and 152 m deep, growing on living rhodoliths. Both stations were rich in massive demosponges, including large Tetractinellids, Petrosia (Petrosia) ficiformis and P. (Strongylophora) vansoesti.

Remarks

This is the third time that the species is recorded, previously only known from the holotype collected in Corsica (Pulitzer-Finali, 1983) and the neotype collected at the Alboran Island (Sitjà & Maldonado, 2014).

Order DESMACELLIDA Morrow & Cárdenas, 2015

Family DESMACELLIDAE Ridley & Dendy, 1886

Genus Dragmatella Hallman, 1917

Dragmatella aberrans (Topsent, 1890)

(Fig. 13; Table 6)

Dragmatella aberrans (Topsent, 1890).

Figure 13: Dragmatella aberrans (Topsent, 1890).

(A) Habitus of CFM-IEOMA-7388/i175 preserved in EtOH. (B–D) SEM images of the skeletal structure of CFM-IEOMA-7388/i175. (B) General view of the skeletal arrangement. (C) Detail of the ectosome. (D) View of the ascending choanosomal tylostyle tracks. (E–E1) Mycalostyles. (F) Raphides with (F1) Detail of the hook-shaped ends and central irregularities.
Table 6:
Comparative characters of representative reports of Dragmatella aberrans.
Specimen Styles Raphides Depth Area
Topsent (1892) 600 180 135–134 Cantabric Sea
Topsent (1928) 600–800 × 9–11.5 70–200 × 12–20 552–1262 Cap Sines (Portugal)
Vacelet (1969) 350–600 × 6–13 150–210 250–324 Cassidaigne (Gulf of Lion)
Pulitzer-Finali (1983) 400–600 × 6–14 200 128–150 Off Calvi (Corsica)
Boury-Esnault, Pansini & Uriz, 1994 315–571–631 × 5–11–16 95–207–260 × 0.4–2–3 485 (Atlantic)
195 (Mediterranean)
Atlantic and Alboran Sea
CFM-IEOMA-7387/i52_b1
This work
349–555–676 × 6–9–13 162–197–222 × 1–2–3 275 SO
St 2
CFM-IEOMA-7388/i175
This work
351–539–651 × 8–11–15 163–193–214 × 1–2–3 138 EB
St 5
DOI: 10.7717/peerj.11879/table-6

Note:

Depth (m), area (SO, Ses Olives; EB, Emile Baudot) and sampling station (St; see Rstudy in Table 1) where these specimens were collected are also shown. Spicule measures are given as minimum-mean-maximum for total length × minimum-mean-maximum for total width. A minimum of 30 spicules per spicule kind are measured, otherwise it is stated. All measurements are expressed in μm. Specimen codes are the reference numbers of the CFM-IEOMA/author collection.

Material examined

CFM-IEOMA-7387/i52_b1, St 2, SO, BT; CFM-IEOMA-7388/i175, St 5, EB, BT.

Description (modified from Hooper & Van Soest, 2002)

Small hollow sponge encrusting on stones or corals. Up to 2 cm in diameter. Whitish grey in life and after preservation in EtOH. Surface smooth, but provided with long thin, pointed fistules (Figs. 13A and 13B).

Skeleton

Ectosome composed of parallel tight tracts of styles, disposed in 4–5 layers of 30–50 µm in thickness (Fig. 13C). The raphides, sometimes grouped in trichodragmata, are scattered in the ectosomal and choanosomal tracts. Choanosome is cavernous (Fig. 13D), with tracts of styles, about 200 µm long, verging from a basal layer towards the ectosome.

Spicules

Megascleres

Styles (Figs. 13E and 13E1) fusiform, tappering towards the head, slightly or abruptly bent. They measure 349–546–676 × 6–10–15 µm.

Microscleres

Raphides (Figs. 13F and 13F1) abundant, straight, with an irregular shaft and one end hook-shaped, occasionally with central swellings. They measure 162–195–222 × 1–2–3 µm.

Ecological notes

Abundant species on sedimentary bottoms, with rests of calcareous shells and corals, found in SO, AM and EB and, to a lesser extent, on trawl fishing grounds of the continental shelf off Mallorca (between 138 and 362 m deep). On the same bottoms other small encrusting sponges such as Hamacantha spp. or Bubaris spp., the pedunculated Rhizaxinella pyrifera (Delle Chiaje, 1828) and Thenea muricata (Bowerbank, 1858), the brachiopod Gryphus vitreus (Born, 1778) and small crustaceans are to be found.

Remarks

The species is easily distinguished by its hollow body and the possession of both styles and raphides. The latter have singular hook-shaped ends, a feature that had not been recorded before, and that is similar to the raphides found in some species of the genus Dragmaxia (Order Axinellida) (Hooper & Van Soest, 2002). No molecular data are available for Dragmatella, but a phylogenetic relationship with Dragmaxia is unlikely, given the possession of styles and the skeletal arrangement of both genera. Therefore, hook-shaped raphide are probably homoplasic.

This is the first report of the species in the Balearic Islands. In the Mediterranean Sea it has been recorded at the Gulf of Lions (Vacelet, 1969), Corsica (Pulitzer-Finali, 1983) and the Alboran Sea (Boury-Esnault, Pansini & Uriz, 1994; Sitjà & Maldonado, 2014). In the North Atlantic Ocean, this species has been recorded at several locations, including the coast of Portugal (Topsent, 1895), the Josephine Bank (Topsent, 1928) and the Cantabric Sea (Topsent, 1890).

Order HAPLOSCLERIDA Topsent, 1928

Family CHALINIDAE Gray, 1867

Genus Haliclona Grant, 1841

Subgenus Soestella De Weerdt, 2000

Haliclona (Soestella) fimbriata Bertolino & Pansini, 2015

Material examined

CFM-IEOMA-7389/i825_1, St 40, EB, ROV.

Ecological notes

The species was spotted regularly at the rhodolith beds of the EB summit, between 134 and 150 m deep. However, it was less abundant and not forming patches, as occurs in some areas of the Gulf of St. Eufemia in the Tyrrhenian Sea, where Bertolino et al. (2015) reported densities of 7.4 ± 0.7 specimens/m2.

This is the second report of the species, previously recorded only at the Gulf of St. Eufemia (southern Tyrrhenian Sea; Bertolino et al., 2015), expanding its distribution range towards the westernmost part of the Mediterranean Sea.

Family PETROSIIDAE Van Soest, 1980

Genus Petrosia Vosmaer, 1885

Subgenus Strongylophora Dendy, 1905

Petrosia (Strongylophora) vansoesti Boury-Esnault, Pansini & Uriz, 1994

Material examined

CFM-IEOMA-7390/i192_A and CFM-IEOMA-7391/i192_B, St 6, EB, BT; CFM-IEOMA-7392/i313_P and CFM-IEOMA-7393/i313_G, St 11, EB, BT; CFM-IEOMA-7394/i351, St 13, EB, BT; CFM-IEOMA-7395/i694, St 34, EB, RD.

Ecological notes

Large amounts of P. (S) vansoesti were collected from various stations in the summit of the EB, suggesting that it is an important species inhabiting Mediterranean seamounts and probably a habitat builder that confers three-dimensionality to the seafloor. The species was found from 116 to 152 m deep, on stations with living and dead rhodoliths and gravels, associated with large sponges such as P. (P.) ficiformis and some tetractinellids and halichondrids. Many groups of invertebrates, such as small crustaceans and echinoderms, were also observed at these stations.

Remarks

This is the first record of the species in the western Mediterranean. The type locality is the Gulf of Cadiz, in the north-eastern Atlantic. In the Mediterranean it has been recorded in marine caves at both the Ionian Sea (Costa et al., 2019) and the Aegean Sea (Gerovasileiou & Voultsiadou, 2012). It has been also recorded at the Levantine Sea, living on rocks at depths shallower than 3 m (Evcen & Çinar, 2012). On the Balearic Islands, the species has only been collected in EB.

Subgenus Petrosia Vosmaer, 1885

Petrosia (Petrosia) raphida Boury-Esnault, Pansini & Uriz, 1994

(Fig. 14; Table 7)

Petrosia (Petrosia) raphida Boury-Esnault, Pansini & Uriz, 1994.

Figure 14: Petrosia (Petrosia) raphida Boury-Esnault, Pansini & Uriz, 1994.

(A) Habitus of CFM-IEOMA-7451/i242, preserved in EtOH. (B) SEM image of the choanosome. (C) Detail of a choanosomal chamber. (D) Oxeas. (E) Young stages of oxeas. (F–F1) Acanthoses raphides. (G) Smooth raphides.
Table 7:
Comparative characters from published records of Petrosia (Petrosia) raphida Boury-Esnault, Pansini & Uriz, 1994 and present work.
Specimen Oxeas Raphides Depth Area
Boury-Esnault, Pansini & Uriz (1994)
Holotype
354–449–499 × 26–32–36 (strongyles) 81–95–108 × 1 580 Gibraltar
Sitjà et al. (2019) 290–500 × 20–25 (rarely as short as 7.5) 75–100 × 1 (some without spines) 530–573 Volcano of Gulf of Cadiz (Pipoca)
CFM-IEOMA-7396/POR406
This work
271–369–432 × 9–13–16 62–78–91 × 1–1–2 134 South-east of Menorca
St 1
CFM-IEOMA-7397/i178_3
This work
242–378–450 × 10–16–19 72–80–89 × 2–3–4 138 EB
St 5
CFM-IEOMA-7451/i242
This work
268–333–380 × 11–14–17 70–80–91 × 1–2–2 101 AM
St 8
CFM-IEOMA-7398/i254_2
This work
300–378–426 × 9–15–19 66–75–86 × 1–2–2 101 AM
St 8
CFM-IEOMA-7399/i305
This work
242–346–394 × 9–15–19 65–75–88 × 1–2–2 118 AM
St 10
CFM-IEOMA-7400/i312_1
This work
349–403–453 × 8–15–19 70–79–95 × 1–2–2 152 EB
St 11
DOI: 10.7717/peerj.11879/table-7

Note:

Depth (m), area (SO, Ses Olives; AM, Ausias March; EB, Emile Baudot) and sampling station (St; see Rstudy in Table 1) where these specimens were collected are also shown. Spicule measures are given as minimum-mean-maximum for total length × minimum-mean-maximum for total width. A minimum of 30 spicules per spicule kind are measured, otherwise it is stated. All measurements are expressed in μm. Specimen codes are the reference numbers of the CFM-IEOMA/author collection.

Material examined

CFM-IEOMA-7396/POR406, St 1, south-east of Menorca, GOC-73; CFM-IEOMA-7397/i178_3, St 5, EB, BT; CFM-IEOMA-7451/i242 and CFM-IEOMA-7398/i254_2, St 8, AM, BT; CFM-IEOMA-7399/i305, St 10, AM, BT; CFM-IEOMA-7400/i312, St 11, EB, BT.

Description

Massive sponges, the largest collected specimen measuring about 4.5 cm in diameter and 2.5 cm in height (Fig. 14A). Whitish in life, beige after preservation in EtOH. Consistency hard, slightly crumbly. Surface rough due to minute conules, although in some specimens this is less obvious. There are 1 to 6 circular oscules of 2–5 mm diameter.

Skeleton

Ectosome forming a detachable crust not evident to the naked eye, tightly adhering to the choanosome, and made of irregular net of polygonal to triangular meshes. Meshes are constituted by one or two spicules. Spongin is present and fully embedded with raphides.

Choanosome (Figs. 14B and 14C) with an isotropic net of pauci-spicular spicule tracts covered by spongin, forming roundish meshes. These meshes are abundantly embedded by raphides. The tracts tend to condense towards the surface, supporting the ectosome.

Spicules

Oxeas (Figs. 14D and 14E): curved, with mucronated ends. Some polyaxonal modification in the shaft and ends may be present. They measure 242–372–450 × 9–15–19 µm, although underdeveloped stages (196–368/3–8 µm) are present. Styles and strongyles of the same length and width as the oxeas, present but scarce.

Raphides (Figs. 14F and 14F1): slightly curved, most minutely spined, although smooth ones are also present (Fig. 14G). They measure 62–77–95 × 1–2–2 µm.

Ecological notes

This species is very common in both AM and EB at the 101–152 m bathymetric range, and has been also found at the same depths off the southern coast of Menorca (Table 7). It can be found as a free-living sponge or growing attached to small fragments of calcareous sediments. However, it is also commonly found as an epibiont of other sponges and rhodoliths. The species seems to prefer massive specimens of Hexadella sp. and Halichondria sp. as substrate.

Remarks

The species is easily recognized due to the presence of characteristic spined raphides, added to other Petrosid features such as the skeletal architecture and the morphology of the oxeas. Remarkably, the specimens described in this study differ from the two previous reports in having much smaller oxeas (see below in brackets). This could be explained by the scarcity of nutrients in waters around the Balearic Islands, the bathymetric range in which the specimens were collected and/or differences in water temperature, seasonal variability and population phenotypes (Simpson, 1978; Valisano et al., 2012). These variables could also be the cause of differences in the morphology of the megascleres already noted by Sitjà et al. (2019) when comparing their material with the holotype. In the specimens from the Gulf of Cadiz (north-eastern Atlantic), strongyles were rare. Instead, megascleres consisted mostly of oxeas with stepped tips and some occasional stylote or strongylote modifications. This last feature is shared with specimens of the Balearic Islands but not with the holotype, whose spicules have mostly strongylote extremities. These differences may be related to variations in nutrient regimes between the Balearic Islands and these areas (Santinelli, 2015).

This is the first record of the species in the Mediterranean, but at a considerably shallower depth (101–152 m) than in the north-eastern Atlantic, where the species was reported at 580 m deep in the Strait of Gibraltar (Boury-Esnault, Pansini & Uriz, 1994) and at 530–575 m deep in the Gulf of Cadiz (Sitjà et al., 2019) (Table 7).

Family PHLOEODICTYIDAE Carter, 1882

Genus Calyx Vosmaer, 1885

Calyx cf. tufa

(Fig. 15; Table 8)

Calyx cf. tufa.

Figure 15: Calyx cf. tufa.

(A) Habitus of CFM-IEOMA-7403/i525 in fresh state. (B) Detail of the ectosome with poral areas. (C) View of a poral area of the ectosome with spherulous cells (arrow). (D) Transversal section of the choanosome. (E) Detail of the reticulation of the choanosome. (F–G) Immature and mature oxeas, with (G1) Detail of the tips of (G).
Table 8:
Comparative characters from Calyx cf. tufa and Calyx tufa Ridley & Dendy, 1886.
Specimen Oxeas External morphology Depth Area
Calyx cf. tufa
CFM-IEOMA-7403/i525
This work
146–170–189 × 6–7–8 Large, massive, roundish. Surface smooth. Stony hard and uncompressible. Ectosomal crust present. Beige with pink tints at the upper side. Whitish beige after EtOH 114 AM
St 24
CFM-IEOMA-7402/i515
This work
140–171–205 × 4–7–9 As the holotype 113 AM
St 23
CFM-IEOMA-7401/i75
This work
132–178–206 × 4–6–9 As the holotype 105 AM
St 3
Calyx tufa
Ridley & Dendy (1886)
holotype
200 × 10 Massive, cake-like. Firm, almost stony, but brittle. Surface smooth but uneven. Dermal membrane (=ectosomal crust) readily peeling off. Vents rather small, circular, flush. Greyish yellow. 219 St Lago, Cape Verde
Topsent (1892) nr Firm but crumbly. Without ectosomal crust due to damaging. Light brown. 300 Cantabrian Sea
DOI: 10.7717/peerj.11879/table-8

Note:

Depth (m), area (AM, Ausias March) and sampling station (St; see Rstudy in Table 1) where these specimens were collected are also shown. Spicule measures are given as minimum-mean-maximum for total length × minimum-mean-maximum for total width. A minimum of 30 spicules per spicule kind are measured, otherwise it is stated. All measurements are expressed in μm. Specimen codes are the reference numbers of the CFM-IEOMA/and author collection. nr, not reported.

Material examined

CFM-IEOMA-7403/i525, St 24, AM, BT; CFM-IEOMA-7401/i75, St 3, AM, BT; CFM-IEOMA-7402/i515, St 23, AM, BT.

Description

Large, massive and semicircular sponges, up to 15 cm in diameter and 5 cm in height (Fig. 15A). Surface smooth to the touch; consistency stony hard and uncompressible. Choanosome slightly friable and cavernous. Color in life beige, with pink tints in the upper side of the body and whitish beige in the lower. It became homogeneous brownish beige after preservation in EtOH. A total of Two to three large and circular oscula are located in the upper side of the body, measuring 1.3 cm in diameter. Ostia grouped in poral areas of the ectosome (Fig. 15B).

Skeleton

The ectosome (Fig. 15C) is formed by a crust of tangential spicules, forming triangular paucispicular meshes that become less dense at the poral areas. Spongin present but not abundant, with a granular appearance due to the presence of spherulous cells filled with granules (Fig 15C, arrow).

The choanosome (Figs. 15D and 15E) is mostly composed of a rather isotropic, unispicular net of spicules.

Spicules

Oxeas (Figs. 15F and 15G): slightly curved, with stepped or slightly mucronate points (Fig. 15G1) and rarely bent in the middle. They measure 132–173–206 × 4–7–9 µm.

Genetics

Sequences of COI Folmer and 28S C1–D2 domains were obtained from the specimen CFM-IEOMA-7403/i525 and deposited in Genbank under the accession numbers MW858349 and MW881149, respectively.

Ecological notes

The species was found only at the summit of AM between 105 and 114 m deep, associated with rhodolith beds. It has also been found amongst with diverse set of sponges, including large Tetractinellids and other sponges such as Hexadella sp., Axinella spp. or P. (P.) raphida, as well as among many other invertebrates typically inhabiting the rhodolith beds, like small crustaceans and echinoderms. The pink coloration of its upper skin is probably caused by symbiotic cyanobacteria, as commonly happens in other Haplosclerids (Rützler, 1990).

Remarks

There are only two reported species of Calyx from the north-eastern Atlantic and the Mediterranean: Calyx nicaeensis (Risso, 1827) and C. tufa (Ridley & Dendy, 1886). The first is the type species of the genus, which is a well-known species characterized by its growing habit (vasiform), blackish color and large size. This species has been widely reported at both the western and eastern Mediterranean in infralittoral and circalittoral bottoms at 3–50 m deep (Trainito, Baldacconi & Mačić, 2020). Calyx cf. tufa clearly differs from C. nicaeensis in morphology (massive vs. vasiform, respectively), genetics (COI: 11 bp difference; 28S: 43 bp difference) and bathymetry (105–114 vs. 3–50 m, respectively). Calyx tufa is only known from its type locality at Cape Verde (Dendy, 1886) and from the Cantabrian Sea (Topsent, 1892). The species that we studied shares many characteristics with C. cf. tufa, including external morphology, consistency, and skeletal architecture. Unfortunately, the only description available is the one provided by Dendy (1886), which is too general and matches with the characters of many other Calyx spp. (e.g., Calyx podatypa de Laubenfels 1934; Calyx magnoculata Van Soest, Meesters & Becking, 2014; Calyx nyaliensis Pulitzer-Finali, 1983). The large distances between the reports of C. tufa and C. cf. tufa, the strong genetic barriers that separate the two records (the Strait of Gibraltar and the Almeria-Oran front), the generalized low dispersive potential of some sponge species (Riesgo et al., 2019; Griffiths et al., 2021) and the difference of habitats, are reasons that may suggest that the species that we report here is different from C. tufa. Moreover, no intermediate geographical findings have been reported, which would be expected if there was conspecificity (Topsent, 1928; Maldonado, 1992; Boury-Esnault, Pansini & Uriz, 1994; Sitjà & Maldonado, 2014; Sitjà et al., 2019). It should be noted that C. cf. tufa is a very large, massive, and easily recognizable sponge, which cannot be easily go unnoticed. However, considering that we did not study the holotype and that no genetic sequences of C. tufa are available, the possible conspecificity of C. cf. tufa with C. tufa cannot be completely assessed. Therefore, future work comparing the holotype of C. tufa may be needed to determinate if both species are different or conspecific. Considering the mentioned lack of data, here we use the more conservative choice by assigning the present record to C. cf. tufa.

Order POECILOSCLERIDA Topsent, 1928

Family MYXILLIDAE Dendy, 1922

Genus Melonanchora Carter, 1874

Melonanchora emphysema (Schmidt, 1875)

(Fig. 16)

Melonanchora emphysema (Schmidt, 1875).

Figure 16: Melonanchora emphysema (Schmidt, 1875).

(A) Habitus of CFM-IEOMA-7404/i573 on fresh state, attached to a rodolith. (B–C) Tylotes. (D–E) Spheranchoras. (F) Anchorate isochela I. (G) Anchorate isochela II.

Synonymised names

Desmacidon emphysema Schmidt, 1875 (genus transfer)

Material examined

CFM-IEOMA-7404/i573, St 31, AM, RD.

Description

Hollow sponge with a detachable, smooth and paper-like ectosome provided with fistulas (Fig. 16A). About 2 cm in diameter. The choanosome is loose and includes sediment. Greyish white in life and after preservation in EtOH.

Skeleton

As in the previous records of the species (Schmidt, 1785; Vacelet, 1969; Pulitzer-Finali, 1983).

Spicules

Megascleres

Tylotes (Figs. 16B and 16C) slightly curved, with roundish ends. Their length tends to be inversely related to their thickness. They measure 359–446–556 × 5–8–11 µm.

Microscleres

Spheranchoras (Figs. 16D and 16E) of usual morphology, but uncommon. They measure 36–40–46 × 14–19–23 µm (n = 11).

Arcuate isochela I (Fig. 16F) with well-developed fimbriae and spatulated and bifid alae. They measure 29–42–47 µm.

Arcuate isochela II (Fig. 16G) similar to isochela I, but with rounded alae. They measure 14–18–21 µm.

Ecological notes

The single specimen was found in AM, on a rhodolith bed between 104 and 138 m deep. It was growing upon a large rhodolith, which was extensively epiphyted by encrusting, massive-encrusting or pedunculated sponges (like Hamacantha sp. or Jaspis sp.) or pedunculated Axinellids.

Remarks

The specimen matches well with the previous records of the species, both in external morphology, spicules and skeletal arrangement. This is the third record of this species in the Mediterranean, where it was recorded in the canyon de Cassidaigne in the Gulf of Lions (Vacelet, 1969) and Corsica (Pulitzer-Finali, 1983). In the North-Atlantic, it has been reported at several localitions: the type locality at Norway (Schmidt, 1875), the east Greenland shelf (Lundbeck, 1905), the Faroe Plateau (Hentschel, 1929) and the north coast of the Iberian Peninsula (Solórzano, 1990). The vast distances between the Mediterranean and the North Atlantic reports (being the closest off northern Iberian Peninsula), and the lack of intermediate reports in well-studied areas such as the Alboran Sea, may indicate that Mediterranean and North Atlantic M. emphysema are different species, as already discussed by Vacelet (1969).

Order POLYMASTIIDA (Morrow & Cárdenas, 2015)

Family POLYMASTIIDAE (Gray, 1867)

Genus Polymastia (Lammarck, 1815)

Polymastia polytylota Vacelet, 1969

(Fig. 17; Table 9)

Polymastia polytylota Vacelet, 1969.

Figure 17: Polymastia polytylota Vacelet, 1969.

(A–B) Habitus of CFM-IEOMA-7405/i810, on fresh state (A), and preserved in EtOH (B). (C–C1) Principal subtylostyles with detail of the tyles in the shaft (arrows). (D) Intermediary tylostyles with (D1) detail of the head. (E) Ectosomal tylostyles with (E1) Detail of the head.
Table 9:
Comparative characters from Polymastia polytylota Vacelet, 1969.
Specimen Principal tylostyles Intermedium tylostyles Ectosomal tylostyles Depth Area
Boury-Esnault, (1987)
Redescription of the Holotype
650–990 × 10–13 210–490 × 7–10 70–180 × 2–5 165–270 Toulon, but also in Corsica
Boury-Esnault, Pansini & Uriz (1994) 668–854–1108 × 5–13–16 276–403–509 × 5–11–13 94–115–143 × 3–3–4 Alboran:480
Atl: 362–485
Alboran Sea and North Atlantic
Pulitzer-Finali (1983) 650–810 × 10–13 210–490 × 7–10 80–120 × 2–3 117 North of Corsica
CFM-IEOMA-7405/i810
This work
438–909–1154 × 8–11–15 308–443–586 × 6–7–9 121–166–200 × 2–3–5 352–465 AM
St 3
DOI: 10.7717/peerj.11879/table-9

Note:

Depth (m), area (AM, Ausias March) and sampling station (St; see Rstudy in Table 1) where these specimens were collected are also shown. Spicule measures are given as minimum-mean-maximum for total length × minimum-mean-maximum for total width. A minimum of 30 spicules per spicule kind are measured, otherwise it is stated. All measurements are expressed in μm. Specimen codes are the reference numbers of the CFM-IEOMA/and author collection.

Material examined

CFM-IEOMA-7405/i810, St 39, AM, ROV.

Description

Rounded sponge, 2 cm high and wide, with two conical papillas (0.5 cm high and 3 mm wide) placed on the upper side of the body (Figs. 17A and 17B). Consistency hard and slightly compressible. Surface smooth to the touch, but microhispid under the stereomicroscope. Cream color before and after preservation in EtOH, with a darker choanosome. The specimen suffered a contraction after collection. In situ the sponge was 4.5 cm in height and 4 cm in width, being looser and with its surface full of visible ostia (Fig. 2A).

Skeleton

As in the previous reports of the species (Vacelet, 1969; Pulitzer-Finali, 1983; Boury-Esnault, 1987; Boury-Esnault, Pansini & Uriz, 1994).

Spicules

Principal tylostyles (Figs. 17C and 17C1): straight and fusiform, with several tyles in the proximal half part of the shaft. They measure 438–909–1154 × 8–11–15 µm.

Intermediary tylostyles (Figs. 17D and 17D1): fusiform, with a rounded head, often showing a vesicle. They measure 308–443–586 × 6–7–9 µm.

Ectosomal tylostyles (Figs. 17E and 17E1): slightly curved. They measure 121–166–200 × 2–3–5 µm.

Ecology notes

Only one specimen collected in the northern part of the AM, between 352 and 465 m deep, on a rocky bottom characterized by enhanced water movement, with several large Phakellia spp, Pachastrella spp and Poecillastra compressa, as well as other Polymastia cf. polytylota. Although the present specimen was the only collected, its easy identification and the other sighthings during ROV transects may suggest that this sponge is quite common in some areas of the Mallorca Channel.

Remarks

The present specimen matches with the previous descriptions of the species in external morphology, skeletal architecture and spicule morphometrics. The only difference is that our specimen has two papillae instead of one. The Fig. 2A shows the first in-situ image of this species.

This is the first record of this species at the Balearic Islands. In the Mediterranean it is known from the type locality at the Gulf of Lions (Vacelet, 1969), the Ligurian Sea (Vacelet, 1969; Pulitzer-Finali, 1983) and the Alboran Sea, while it has been also reported at the Gulf of Cadiz in the north-eastern Atlantic (Boury-Esnault, Pansini & Uriz, 1994).

Genus Pseudotrachya Hallmann, 1914

Pseudotrachya hystrix (Topsent, 1890)

(Fig. 18; Table 10)

Pseudotrachya hystrix (Topsent, 1890).

Figure 18: Pseudotrachya hystrix (Topsent, 1890).

(A) Habitus of CFM-IEOMA-7407/i613 on fresh state. (B–B1) SEM images of the skeletal structure. (po) Palisade of oxeas. (C–C1) Anisoxeas. (D) Microxeas.
Table 10:
Comparative characters from Pseudotrachya hystrix (Topsent, 1890).
Specimen Anisoxeas Microxeas Depth (m) Area
Topsent (1892)
holotype
up to 7,000 × 70 185 × 6 318 and 454 Azores
Topsent (1928) nr nr 650–914 Azores
Sarà (1959) 4,000–5,000 × 35–45 150–240 × 3–5 100 Tyrrenhian sea
Boury-Esnault, Pansini & Uriz (1994) 2,000–3,400–4,300 × 18–44–63 200–235–330 × 5–6–7 153–568 Alboran Sea,
Vacelet (1969)
(Various specimens)
St15: 1,000–1,250 × 22–30
St23:>2,000 × 30–35
St34: 1,600–6,600 × 18–40
St46:1,100–4,500 × 20–60
110–320 × 3–5
Stylote modifications
St 15: 180
St 23: 210–240
St 34: 270
St 46: 450–550
St 15: Cassidaigne
St 23: Corse
St 34: Cassidaigne
St 46: Cassidaigne
CFM-IEOMA-7406/i303_A
This work
834–1,689–3,358 × 10–25–42 156–185–217 × 4–5–6 231–302 AM
St 19
CFM-IEOMA-7407/i613
This work
768–2,088–3,402 × 18–32–45 152–203–270 × 3–5–6 195–222 AM
St 32
DOI: 10.7717/peerj.11879/table-10

Note:

Depth (m), area (AM, Ausias March) and sampling station (St; see Rstudy in Table 1) where these specimens were collected are also shown. Spicule measures are given as minimum-mean -maximum for total length × minimum-mean -maximum for total width. A minimum of 30 spicules per spicule kind are measured, otherwise it is stated. All measurements are expressed in μm. Specimen codes are the reference numbers of the CFM-IEOMA/and author collection. nr, not reported.

Material examined

CFM-IEOMA-7406/i303_A, St 19, AM, RD; CFM-IEOMA-7407/i613, St 32, AM, RD.

Description

Roundish and pad-like encrusting sponge, up to 2 cm diameter and 3 mm in height (Fig. 18A). Coloration beige in life and whitish after preservation in EtOH. Very hispid surface. Consistency hard and only slightly compressible. No papillae, oscula and ostia inconspicuous.

Skeleton (modified from Plotkin, Gerasimova & Rapp, 2012)

Single layered cortex (palisade of microxeas). Main choanosomal skeleton of principal anisoxeas radially arranged, echinating the surface and auxiliary choanosomal skeleton of microxeas (Figs. 18B and 18B1).

Spicules

Anisoxeas (Figs. 18C and 18C1): straight and robust, with stepped ends. Intermediary stages between oxeas and styles present. Anisoxea size differs between specimens, measuring 834–1689–3358 × 10–25–42 µm in specimen i303 and 768–2088–3402 × 18–32–45 µm in specimen i613. Small and immature anisoxeas also present, but very scarce, about 500/10 µm.

Microxeas (Fig. 18D): fusiform and measuring 156–185–217 × 4–5–6 µm in specimen i303 and 152–203–270 × 3–5–6 in specimen i613.

Ecology notes

In addition to the two specimens described above, several other P. hystrix were collected from rocky slopes of AM and EB, between 195 and 302 m deep, suggesting that this species could be quite common in the Mallorca Channel seamounts. The species is found at rocky slopes, together with other small encrusting sponges such as Hamacantha spp., Bubaris spp. and the Hexactinellid Tretodyctium sp.

Remarks

This is a well-known species, characterized by their enormous megascleres with unequal tips (oxeote to stylote), and their small microxeas. Variations in the size of megascleres have been previously documented and may be related to ecological factors such as depth, nutrient availability, or temperature (Maldonado et al., 1999). However, due to their size, the largest megascleres were mostly broken, which could be a reason for the lack of reports on sizes 5,000–7,000 µm (Table 10).

This is the first record of the species in the Balearic Islands, expanding its geographical distribution in the Mediterranean, where it was previously reported at the Tyrrhenian Sea (Sarà, 1959), the Ligurian Sea (Pulitzer-Finali, 1983), the Gulf of Lions (Vacelet, 1969) and the Alboran Sea (Boury-Esnault, Pansini & Uriz (1994)).

Order TETHYIDA Morrow & Cardenas, 2015

Family HEMIASTERELLIDAE Lendenfeld, 1889

Genus Hemiasterella Carter, 1879

Hemiasterella elongata Topsent, 1928

Material examined

CFM-IEOMA-7408/i149_4, St 7, EB, RD; CFM-IEOMA-7409/i337, St 11, EB, BT; CFM-IEOMA-7410/i531, St 24, AM, BT; CFM-IEOMA-7411/POR1066, St 36, south-western Cabrera Archipelago, GOC-73.

Ecological notes

This species was found at meshophotic bottoms, between 109 and 152 m deep, generally associated with rhodolith beds, or areas with dead rhodoliths on the summits of EB and AM, but also sporadically at the same depths on trawl fishing grounds of the continental shelf of Mallorca.

Remarks

This is the third record of the species and the third for the Mediterranean, where it was only known from the Alboran Sea (Sitjà & Maldonado, 2014). It is also the third report worldwide, considering the type locality at Cabo Verde in the eastern Atlantic (Topsent, 1928).

Class HEXACTINELLIDA Schmidt, 1870

Subclass HEXASTEROPHORA Schulze, 1886

Order LYSSACINOSIDA Zittel, 1877

Family ROSSELLIDAE Schulze, 1885

Subfamily LANUGINELLIDAE Gray, 1872

Genus Lanuginella Schmidt, 1870

Lanuginella pupa Schmidt, 1870

(Fig. 19; Table 11)

Lanuginella pupa Schmidt, 1870.

Figure 19: Lanuginella pupa Schmidt, 1870.

(A) Habitus of CFM-IEOMA-7413/i286_2 (left), CFM-IEOMA-7412/i286_1 (middle), i286_3/CFM-IEOMA-7414 (right) preserved in EtOH. (B–I) SEM images of spicules from CFM-IEOMA-7412/i286_1. (B) Choanosomal diactine with (B1) detail of the four central tubercles. (C) Choanosomal hexactine. (D) Hypodermal pentactine. (E) Stauractine. (F–G) Atriala hexactines with (G1) Detail of the spines of (G). (H) Discohexaster. (I) Agglomeration of discohexasters, with a strombiloplumicome (sp) beneath.
Table 11:
Comparative characters from Lanuginella pupa Schmidt, 1870.
Parenchimalia Dermalia Gastralia Hexasters
Specimen Choanosomal
Diactine
Choanosomal
Hexactine
Hypodermal Pentactine Other Atrial hexactine Discohexaster Strombiplumicomes Depth Area
Schmidt (1870)
holotype
nr Cape Verde
(Atlantic Ocean)
Schulze (1897) nr nr nr Stauractines: 160–200 nr 32–80–100 40 201 Little Ki Island (Banda Sea)
Ijima (1904)
Several specimens
up to 4,000 × 22 variable in size, up to 2,000 × 30
(as oxyhexactine)
1,000 × 34 Stauractines
rarely tauactines, 220–330 (average length) × 7
220–330 × 7 40–90 34–76 183–572 Döketsba, Okinose, Mochiyama (Japan), Vries Island (Vries Strait, Pacific Ocean)
Okada, 1932 3,500–5,000 × 100 3,000 × 60 nr nr nr 45–80 50 180 Kagoshima Gulf (Eastern China Sea)
Burton, 1956 nr
Sitjà & Maldonado (2014) 325–3,000 × 4–7 250–850 × 6–13 Perp: 170–850 × 4–10 Prox:
242–950 × 8–12
Abundant stauractines, scarce pentactins,
tauactins and hexactins
43–140 × 2–6
46–150 × 2–6 30–70 nf 690 Gulf of Cadiz (Mud volcano, North Atlantic Ocean)
CFM-IEOMA-7412/i286_1
This work
586–1,900 × 7–14 (n = 6) 664–983 × 10–25 (n = 3) Perp: 175–343–435 × 6–13–20 (n = 13)
Prox:323–777 × 7–19 (n = 3)
Stauractins: 61–91 × 3–5 (n = 5)
Hexactins:
Perp: 101 × 6 (n = 1)
Prox: 151 × 7 (n = 1)
Dist: 105 × 6
(n = 1)
Paratetractins: 77–4 (n = 1)
Prox: 108–113 × 5–6
(n = 2)
Dist: 70–95 × 4–7 (n = 2)
Perp: 71–150 × 4–7 (n = 9)
43–57–70 (n = 12) 20–38 (n = 5) 220–275 AM
St 18
CFM-IEOMA-7413/i286_2
This work
245–1,726–2,586 × 3–9–12 (n = 19) 492–920 × 16–20 (n = 4) Perp: 242–519 × 8–15 (n = 7) Stauractins: 79–132 × 3–5 (n = 5)
Hexactins:
Perp: 68–110 × 2–5 (n = 2)
Prox: 107 × 4
(n = 1)
Dist: 97 × 5 (n = 1)
Perp: 83–145 × 3–6 (n = 7)
49–76 (n = 7) nf 220–275 AM
St 18
CFM-IEOMA-7414/i286_3
This work
528–1,533–2,611 × 3–9–15 349–926 × 10–19 (n = 5) Perp: 137–437 × 9–17 (n = 8)
Prox: 516–831 × 11–14 (n = 5)
Stauractins: 67–91–111 × 3–4–5 (n = 13)
Hexactins: n.f.
Prox: 119–159 × 4–6 (n = 5)
Dist: 79–102 × 4–6 (n = 5)
Perp: 70–96 × 4–6 (n = 5)
45–53–62 (n = 10) 20 (n = 1) 220–275 AM
St 18
DOI: 10.7717/peerj.11879/table-11

Note:

Depth (m), area (AM, Ausias March) and sampling station (St; see Rstudy in Table 1) where these specimens were collected are also shown. Spicule measures are given as minimum-mean-maximum for total length × minimum-mean-maximum for total width. A minimum of 30 spicules per spicule kind are measured, otherwise it is stated. All measurements are expressed in μm. Specimen codes are the reference numbers of the CFM-IEOMA/and author collection. nr, not reported; nf, not found.

Material examined

CFM-IEOMA-7412/i286_1, CFM-IEOMA-7413/i286_2 and CFM-IEOMA-7414/i286_3, St 18, AM, RD.

Description

Tubular (CFM-IEOMA-7412/i286_1) to calyx-like (CFM-IEOMA-7413/i286_2 and CFM-IEOMA-7414/i286_3) sponges (Fig. 19A), up to 4 cm high and 2 cm in diameter. Surface smooth, but slightly hispid at localized areas. Fragile consistency and soft touch. Dirty white color in life and white after preservation in EtOH. All the three specimens present a single, circular oscule at the upper part of the body. One of the calyx-like specimens (CFM-IEOMA-7413/i286_2) has a minute and short peduncle.

Skeleton

As usual for the species (see Ijima, 1904; Tabachnick, 2002 and Sitjà et al., 2019 for detailed descriptions)

Spicules

Choanosomal diactines (Fig. 19B): long and slim, slightly sinuous, with four vestigial tubercles in the center (Fig. 19B1), which may have swelings all over the shaft and spines on their tips. They measure 245–1611 × 3–15 µm.

Choanosomal hexactines (Fig. 19C): with actines of different lengths, sometimes sinuous. They measure 349–983 × 10–25 µm (n = 12).

Hypodermal pentactines (Fig. 19D): with a ray reduced to a stump or absent. Proximal ray much larger than the others and perpendicularly arranded. Rays are smooth or slightly rugose. Proximal ray measuring 242–950 × 7–19 µm (n = 8) and perpendicular rays measuring 137–850 × 4–20 µm (n = 28).

Stauractines (Fig. 19E): with four actines perpendicularly arranged one another in the same plane. They are straight or slightly curved, strongly spined, with roundish tips. They measure 61–111 × 3–5 µm (n = 23).

Dermal hexactines (not shown): uncommon. Rugose, with the proximal ray slightly longer than the distal one. Overall measures: proximal rays 151 × 7 µm (n = 1), distal rays 105 × 6 µm (n = 1) and perpendicular rays 68–110 × 2–6 µm (n = 3).

Paratetractin: only a single spicule observed, measuring77/4 (n = 1).

Atrialia hexactines (Figs. 19F and 19G): common. Slightly rough to smooth. Overall measures: proximal rays 107–159 × 4–6 µm (n = 8), distal rays 70–102 × 4–7 µm (n = 8) and perpendicular rays 70–150 × 3–7 µm (n = 22).

Discohexasters (Fig. 19H): rather uncommon. Some with underdeveloped, twisted rays. They measure 43–76 µm (n = 29).

Strobiloplumicomes (Fig. 19I): very rare and not found in specimen CFM-IEOMA-7413/i286_2. They measure: 20–38 µm (n = 6).

Ecological notes

Species found only at one station located in a rocky slope at SO, between 220 and 275 m deep. It was associated with fossil ostreid reefs and carbonated rocks, together with other encrusting sponges like Hamacantha sp., Bubaris sp., and Jaspis sp.

Remarks

This poorly-known species is the single representative of the genus Lanuginella, reported at several distant locations around the world: Kagoshima Gulf at the Sea of China (Okada, 1932), Ki Island at the Sea of Banda (Schulze, 1897). In the northern Atlantic it was recorded at Cabo Verde (Schmidt, 1870), the Gulf of Cadiz (Sitjà et al., 2019) and the Strait of Gibraltar (Topsent, 1895). This is the first record of the species in the Mediterranean Sea, increasing its already wide distribution. However, a revision of the species is needed, and it is likely that such a cosmopolitan distribution may indicate that L. pupa represents a species complex. However, deep-sea species tend to be more widely distributed than shallow ones, probably because of the uniformity of in the environmental conditions (McClain & Hardy, 2010). A detailed examination of worldwide specimens, combined with molecular methods, may shed more light on it.

Discussion

Biogeography and seamount singularity

The present study increases the knowledge of the sponge diversity of the Mediterranean seamounts. We describe a new genus, three new species, and 15 new geographical reports, including two new reports for the Mediterranean Sea. This study also highlights Foraminospongia balearica sp. nov. as one of the most common sponges at AM and EB, being large and easily distinguishable. This species was never recorded at other previously explored Mediterranean seamounts or ridges of a similar depth range, such as the Seco de los Olivos or the Alboran Ridge, whose sponge fauna has been already studied (Sitjà & Maldonado, 2014; Würtz & Rovere, 2015; De la Torriente et al., 2018). Therefore, the Mallorca Channel seamounts may be considered unique faunal refuges, appealing to what is called the “Seamount endemism hypothesis” (De Forges, Koslow & Poore, 2000), which suggests that geographical separation of seamounts is reflected by genetic isolation of their fauna, which promotes speciation by vicariance. This hypothesis has been questioned, as some works have shown that benthic fauna (including sponges) is well connected among isolated seamounts (Samadi et al., 2006; Ekins et al., 2016). However, others have shown structured populations between seamounts (Castelin et al., 2010), or between seamounts and the continental shore populations (Crochelet et al., 2020). Other authors suggest that there is a mixture of panmictic and structured populations, largely dependent on the characteristics of the single species nature (Rogers, 2018). If we consider that the dispersal of sponges tend to be very limited (Maldonado, 2006; Riesgo et al., 2019; Shaffer et al., 2020; Griffiths et al., 2021), it is plausible that certain seamount sponge populations are highly structured. This limitation in the dispersal may be enhanced in isolated seamounts or in those with peculiar or unique ecological characteristics. In this sense, both AM and EB have very shallow summits and are placed in an area of special oligotrophy (e.g., Bosc, Bricaud & Antoine, 2004; Uitz et al., 2012). The nearest habitat with similar features is the continental shelf of the Balearic promontory, although these areas tend to be under the impact of bottom-trawling (Farriols et al., 2017; Ordines et al., 2017), with the consequent impoverishment of benthic communities (Jennings & Kaiser, 1998). In fact, most of the species of SO, AM and the EB had not been found at the continental shelf of the Balearic Islands (Bibiloni, 1990; 1993; Grinyó et al., 2018; Santín et al., 2018), except for Phakellia robusta, P. hirondellei (Santín et al., 2018), Petrosia (Petrosia) raphida, and Hemiasterella elongata (this work).

The particular conditions of the Balearic Islands, extreme oligotrophy, geographical isolation, low fishing pressure and heterogeneity of habitats (Quetglas et al., 2012; Massutí et al., 2014) suggest this area is a hotspot of sponge diversity, with much of its fauna still unknown, especially at depth below 90 m (Bibiloni, 1990; Santín et al., 2018; Díaz et al., 2020). In recent years, this high diversity has been evidenced by the presence of rich benthic assemblages (Ordines & Massutí, 2009; Barberá et al., 2012; Ordines et al., 2011), as well as by a high number of new species and new geographical reports (e.g., Kovačić, Ordines & Schliewen, 2017; Kovacic et al., 2019; Guzzetti et al., 2019; Ordines et al., 2019; Díaz et al., 2020). Thus, there is a need to find out which sponge species inhabit those waters and how much do they contribute to the benthic biomass. Sponges are key components of the benthic ecosystems, playing important biogeochemical roles (De Goeij et al., 2013) and serving as food or refugee to many other animals (Maldonado et al., 2016). Future works should characterize those benthic habitats of the continental shelf and slope around the Balearic Islands that are potentially similar to those of the Mallorca Channel seamounts (e.g., non-impacted sedimentary and rocky bottoms with rhodoliths and gravels located between 90 and 150 m deep and rocky slopes down to 400–500 m deep). Then, both biocenosis should be compared to confirm the singularity of the habitats of the Mallorca Channel seamounts.

Integrative taxonomy

The generalized lack of distinctive characters has caused sponges to be one of the most difficult groups to classify. This difficulty is also reflected by sponge phylogenetic relationships, with polyphyletic taxa present in all the levels of the Linnean classification (e.g., Cárdenas, Perez & Boury-Esnault, 2012; Díaz et al., 2020). Thus, the use of both morphology and molecular markers is central to the improvement of the knowledge of this group of organisms. Following this approach, here we have proposed the new genus Foraminospongia to be erected in the family Hymerhabdiidae, supported by the two new species Foraminospongia balearica sp. nov. and Foraminospongia minuta sp. nov., confirmed by morphological traits and both COI and 28S markers. On the other hand, the species Heteroxya cf. beauforti has shown no variability in its COI sequence relative to its North Atlantic congenerics, which highlights the importance of morphology and the need to combine both approaches. The COI is known to be a low-resolution marker to discriminate species of sponges (Wörheide 2006), so we also sequenced the more variable 28S marker. However, no 28S sequences are currently available in any database for comparison with the other Heteroxya spp.: this issue should thus be adressed in the future.

A key subject in sponge taxonomy is the robustness of the skeletal characters as a species diagnostic tool, and how reliable they are for discriminating species and populations. Reliable discrimination is further complicated by the fact that skeletal elements may change depending on environmental conditions such as temperature, depth, or nutrient concentration; skeletal elements may also change due to intraspecific plasticity, overall modifying length, width, morphology, and even their presence or absence (Cárdenas, Perez & Boury-Esnault, 2012; Abdul Wahab et al., 2020). No consensus has ever been reached to consider a given morphological deviation as enough evidence to erect a new species, a fact that remains arbitrary. We have found differences in the spicular morphometry between the specimens of the Balearic Islands and specimens of other areas of the Mediterranean and the North Atlantic Ocean; these differences have been described here for most of the species to some extent. Since the dispersive potential, long-distance connectivity, and speciation of sponges are poorly understood, most of the diagnosis in the present work were performed under a conservative approach, only proposing new species when we found solid morphological evidence. Taking this into account, factors like vast geographical distances, presence of oceanographic barriers or minor morphometric differences were not considered enough evidence for species delimitation. In the case of Calyx cf. tufa, its potential conspecificity with the North Atlantic species C. tufa cannot be discarted. We did not get access to any material of C. tufa, and no sequences are available for comparison; moreover, the original description is too vague and general. However, as stated above, the absence of any intermediate records of such a big, conspicuous, and easily recognizable sponge is noreworthy. Also, the recorded depths of C. tufa for the Atlantic are much deeper than those for C. cf. tufa (219 and 300 m vs. 105–114 m). Future work is need to clarify if both species are synonyms, or if C. cf. tufa is a new species for science.

Supplemental Information

List of sequences used in this study by alphabetical order.

DOI: 10.7717/peerj.11879/supp-1

The 28S sequences.

DOI: 10.7717/peerj.11879/supp-2

The COI sequences.

DOI: 10.7717/peerj.11879/supp-3

Raw data from spicule measurements of Melonanchora emphysema specimens.

DOI: 10.7717/peerj.11879/supp-4

Raw data from spicule measurements of Heteroxya cf. beauforti specimens.

DOI: 10.7717/peerj.11879/supp-5

Raw data from spicule measurements of Paratimea massutii sp. nov. specimens.

DOI: 10.7717/peerj.11879/supp-6

Raw data from spicule measurements of Axinella spatula specimens.

DOI: 10.7717/peerj.11879/supp-7

Raw data from spicule measurements of Foraminospongia minuta sp. nov. specimens.

DOI: 10.7717/peerj.11879/supp-8

Raw data from spicule measurements of Petrosia raphida specimens.

DOI: 10.7717/peerj.11879/supp-9

Raw data from spicule measurements of Dragmatella aberrans specimens.

DOI: 10.7717/peerj.11879/supp-10

Raw data from spicule measurements of Foraminospongia balearica sp. nov. gen. nov. specimens.

DOI: 10.7717/peerj.11879/supp-11

Raw data from spicule measurements of Pseudotrachya hystrix specimens.

DOI: 10.7717/peerj.11879/supp-12

Raw data from spicule measurements of Lanuginella pupa specimens.

DOI: 10.7717/peerj.11879/supp-13

Raw data from spicule measurements of Calyx cf. tufa specimens.

DOI: 10.7717/peerj.11879/supp-14

Raw data from spicule measurements of Polymastia polytylota specimens.

DOI: 10.7717/peerj.11879/supp-15
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