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Pei Liew
PeerJ Reviewer
30 Points

Contributions by role
Reviewer 30

Contributions by subject area
Bioinformatics
Cell Biology
Molecular Biology
Oncology
Dermatology
Drugs and Devices
Immunology
Pharmacology

Pei Xiong Liew

PeerJ Reviewer

Summary

Pei X Liew has two concurrent roles. He is a senior research scientist (research faculty) at the Medical College of Georgia. His project involves imaging Kindlin-3 to determine its role in regulating integrin-mediated crawling and adhesion in murine neutrophils in real time using the multiphoton microscope.
He is also the Director of the Intravital Microscopy Core Facility at the Immunology Center of Georgia (IMMCG).

Pei X Liew attained his PhD under Dr. Paul Kubes at the University of Calgary in Canada. His Postdoctorate research fellowship was completed at Harvard Medical School Under Dr. Tanya Mayadas. Pei Xiong's expertise is in Immunology and he specializes in using Intravital Microscopy to visualize the behavior and function of immune cells in live mice using Spinning Disk Confocal or Multiphoton microscopes. Microscopy techniques are supplemented by flow cytometry and scRNAseq transcriptomics to provide a well-rounded approach to study hypothesis-driven research.

Anatomy & Physiology Cell Biology Immunology Infectious Diseases Molecular Biology Pharmacology

Past or current institution affiliations

University of Calgary
Harvard University
Medical College of Georgia

Work details

Senior Research Scientist/Director Intravital Microscopy Core

Medical College of Georgia
August 2023
Immunology Center of Georgia
My current project involves studying the role of Kindlin-3 and integrins in regulating crawling and adhesion in murine neutrophils in real time using a combination of intravital microscopy, multi-panel flow cytometry and scRNAseq. I am also concurrently the Director of the Intravital Microscopy Core Facility at the Immunology Center of Georgia (IMMCG). I am in charge of overseeing daily usage of the microscope in the core facility, training personnel and providing scientific expertise to resolve hypothesis driven questions for other principal investigators.

Master of Science

University of Calgary
January 2008 - August 2010
Department of Biological Sciences
Project involves the design and production of a bacteria system that displays functional enzymes on the cell surface. Genetic design, PCR, DNA isolation, plasmid construction, transformation were all used to genetically modify bacteria to anchor biotechnologically relevant enzymes on the cell surface. Western blotting and immunofluorescence structured illumination microscopy was used to spatially resolve where expressed enzymes were located on the cell.

PhD Candidate

University of Calgary
September 2010 - April 2017
Snyder Institute of Chronic Diseases
PhD (Immunology): Trained with Dr. Paul Kubes with a focus on in vivo pharmacology, animal research (mice), intravital imaging, innate immunity, inflammation and chemokine/cytokine function. My PhD research project involves imaging the immune response of innate immune cells following hepatic sterile injury using live mice and documenting the functional role of these cells during inflammation and physiological healing of the injury. The mechanistic regulation of this process occurred through production of various chemokines and cytokines. My results were published as first author in top journals such as Immunity and Physiological Reviews.

Postdoctoral Research Fellow

Harvard University
February 2018 - July 2023
Department of Pathology/Brigham and Women's Hospital
Postdoctoral research project with Dr. Tanya Mayadas at Harvard University is a combination of immunology, in vivo pharmacology and scRNAseq using mouse models of disease. Current project involves observing the behavior of various types of splenic immune cells in live mice and how these cells respond to both infectious and non-infectious stimuli during health and disease. Furthermore, the project involves understanding the roles of chemokines, cytokines and adhesion molecules in regulating this immune cell response. This project expands my knowledge of innate and adaptive immunology with further training in advanced microscopy, flow cytometry and scRNAseq skills.

Websites

  • LinkedIn