Academic Editor

Paul S. Brookes


Summary

Associate Professor of Anesthesiology at University of Rochester Medical Center. PhD in Biochemistry from Cambridge University (UK) and post-doctoral training at the University of Alabama at Birmingham. Research program in mitochondria, cardiac ischemia, and cardioprotective therapies.

Biochemistry Cardiology Cell Biology

Institution affiliations

Work details

Professor

University of Rochester
Anesthesiology
Our broad research interest is cardioprotection against ischemia-reperfusion (IR) injury. We are particularly interested in mechanisms of protection in ischemic preconditioning (IPC), and anesthetic preconditioning (APC) and the role of mitochondria and metabolism in these processes. A variety of model systems are used, including: isolated heart mitochondria, Langendorff perfused mouse hearts, isolated adult mouse cardiomyocytes, in-vivo mouse coronary artery occlusion, and H9c2 cardiomyocytes in cell culture. We also use many biochemical techniques to investigate mitochondrial function including: mitochondrial respiration & membrane potential assays, electrode and fluorescence based measurements of reactive oxygen species (ROS) and nitric oxide, analysis of protein post-translational modifications (phosphorylation, acetylation, S-nitrosation, nitroalkylation) by 2D gels, western blotting, mass spectrometry (via the proteomics core), spectrophotometry, fluorescence spectroscopy, Seahorse XF24 and XF96 extracellular flux analysis, chemical synthesis and development of novel small molecule therapeutics, and metabolomics. We maintain several lines of engineered mice for these studies

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