
PeerJ Editor & Author
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Paul S. Brookes
PeerJ Editor & Author
Summary
Professor of Anesthesiology at University of Rochester Medical Center. PhD in Biochemistry from Cambridge University (UK) and post-doctoral training at the University of Alabama at Birmingham. Research program in mitochondria, cardiac ischemia, and cardioprotective therapies.
Editing Journals
PeerJ - the Journal of Life & Environmental Sciences
Past or current institution affiliations
Work details
Professor
University of Rochester
Anesthesiology
Our broad research interest is cardioprotection against ischemia-reperfusion (IR) injury. We are particularly interested in mechanisms of protection in ischemic preconditioning (IPC), and anesthetic preconditioning (APC) and the role of mitochondria and metabolism in these processes. A variety of model systems are used, including: isolated heart mitochondria, Langendorff perfused mouse hearts, isolated adult mouse cardiomyocytes, in-vivo mouse coronary artery occlusion, and H9c2 cardiomyocytes in cell culture. We also use many biochemical techniques to investigate mitochondrial function including: mitochondrial respiration & membrane potential assays, electrode and fluorescence based measurements of reactive oxygen species (ROS), analysis of protein post-translational modifications (esp. acetylation & S-nitrosation) by 2D gels, western blotting and mass spectrometry, spectrophotometry, fluorescence spectroscopy, Seahorse XF extracellular flux analysis, chemical synthesis and development of novel small molecule therapeutics, and LC-MS based metabolomics. We maintain several lines of engineered mice for these studies.
Websites
PeerJ Contributions
April 3, 2014
Paul S. Brookes
March 5, 2013
Andrew P. Wojtovich, Sergiy M. Nadtochiy, William R. Urciuoli, Charles O. Smith, Morten Grunnet, Keith Nehrke, Paul S. Brookes
Academic Editor on
November 12, 2021
Eduardo Cienfuegos-Pecina, Diana P. Moreno-Peña, Liliana Torres-González, Diana Raquel Rodríguez-Rodríguez, Diana Garza-Villarreal, Oscar H. Mendoza-Hernández, Raul Alejandro Flores-Cantú, Brenda Alejandra Samaniego Sáenz, Gabriela Alarcon-Galvan, Linda E. Muñoz-Espinosa, Tannya R. Ibarra-Rivera, Alma L. Saucedo, Paula Cordero-Pérez