PeerJ Editor & Author
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Paul S. Brookes
PeerJ Editor & Author
Summary
Professor of Anesthesiology at University of Rochester Medical Center. PhD in Biochemistry from Cambridge University (UK) and post-doctoral training at the University of Alabama at Birmingham. Research program in mitochondria, cardiac ischemia, and cardioprotective therapies.
Editorial Board Member
PeerJ - the Journal of Life & Environmental Sciences
Past or current institution affiliations
Work details
Professor
University of Rochester
Anesthesiology
Our broad research interest is cardioprotection against ischemia-reperfusion (IR) injury. We are particularly interested in mechanisms of protection in ischemic preconditioning (IPC), and anesthetic preconditioning (APC) and the role of mitochondria and metabolism in these processes. A variety of model systems are used, including: isolated heart mitochondria, Langendorff perfused mouse hearts, isolated adult mouse cardiomyocytes, in-vivo mouse coronary artery occlusion, and H9c2 cardiomyocytes in cell culture. We also use many biochemical techniques to investigate mitochondrial function including: mitochondrial respiration & membrane potential assays, electrode and fluorescence based measurements of reactive oxygen species (ROS), analysis of protein post-translational modifications (esp. acetylation & S-nitrosation) by 2D gels, western blotting and mass spectrometry, spectrophotometry, fluorescence spectroscopy, Seahorse XF extracellular flux analysis, chemical synthesis and development of novel small molecule therapeutics, and LC-MS based metabolomics. We maintain several lines of engineered mice for these studies.
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PeerJ Contributions
April 3, 2014